--- gene_symbols: - MtCCD1 gene_symbol_long: carotenoid cleavage dioxygenase 1 gene_model_pub_name: CAR57918.1 gene_model_full_id: medtr.A17_HM341.gnm4.ann2.Medtr6g034835 confidence: 5 curators: - Steven Cannon phenotype_synopsis: RNA interference (RNAi) was used to repress a M. truncatula CCD1 gene in hairy roots colonized by the arbuscular mycorrhizal (AM) fungus Glomus intraradices. The normal AM-mediated accumulation of apocarotenoids (C13 cyclohexenone and C14 mycorradicin derivatives) was reduced in repressed plants; mycorradicin derivatives were reduced to 3% to 6% of the controls and the cyclohexenone derivatives were reduced to 30% to 47%. The RNAi roots turned a yellow-orange color because of C27 apocarotenoid accumulation (the probable substrate of the CCD1 enzyme). More degenerating arbuscules was observed in RNAi roots. traits: - entity_name: carotene content entity: TO:0000289 - entity_name: root entity: PO:0009005 references: - citation: Floss, Schliemann et al., 2008 doi: 10.1104/pp.108.125062 pmid: 18790999 - citation: Oellrich, Walls et al., 2015 doi: 10.1186/s13007-015-0053-y pmid: 25774204