Run of program pandagma-pan.sh, version 2023-08-28 Run started at: Sat Sep 2 17:32:32 CDT 2023 Run ended at: Sat Sep 2 17:58:16 CDT 2023 Sequence type: nucleotide Parameter value clust_iden 0.95 clust_cov 0.50 consen_iden 0.80 extra_iden 0.80 mcl_inflation 1.6 strict_synt 1 dagchainer_args -g 10000 -M 50 -D 200000 -E 1e-5 -A 6 -s out_dir_base out pctl_low 25 pctl_med 50 pctl_hi 75 consen_prefix Vigna.pan2 annot_str_regex ([^.]+\.[^.]+\.[^.]+\.[^.]+)\..+ order_method alignment preferred_annot IT97K-499-35.gnm1.ann2 work_dir /project/legume_project/steven.cannon/pandagma/Vigna/../work_Vigna_7_5 Output directory for this run: out_Vigna_7_5 Statistic value == Initial clusters (containing only genes within synteny blocks) Cluster file 06_syn_pan.clust.tsv num_of_clusters 29526 largest_cluster 14 modal_clst_size 7 num_at_mode 22969 seqs_clustered 187669 == Augmented clusters (unanchored sequences added to the initial clusters) Cluster file 12_syn_pan_aug.clust.tsv num_of_clusters 30200 largest_cluster 28 modal_clst_size 7 num_at_mode 23402 seqs_clustered 191845 == Augmented-extra clusters (with sequences from extra annotation sets) The pctl25 set consists of orthogroups with at least 4 genes per OG (>= 12 * 25/100 sets). Cluster file 18_syn_pan_aug_extra.clust.tsv num_of_clusters 30200 largest_cluster 76 modal_clst_size>=4 12 num_at_mode>=4 12370 seqs_clustered 308214 == Sequence stats for CDS files Class: seqs min max N50 ave annotation_name Main: 28297 90 16188 1599 1240.0 vigun.CB5-2.gnm1.ann1 Main: 31948 90 16278 1602 1214.9 vigun.IT97K-499-35.gnm1.ann2 Main: 28461 90 16278 1602 1238.1 vigun.Sanzi.gnm1.ann1 Main: 28545 90 16272 1605 1239.5 vigun.Suvita2.gnm1.ann1 Main: 27742 90 16272 1602 1254.2 vigun.TZ30.gnm1.ann2 Main: 28562 90 16272 1602 1239.6 vigun.UCR779.gnm1.ann1 Main: 27723 90 16272 1605 1254.2 vigun.ZN016.gnm1.ann2 Extra: 29773 90 16278 1611 1238.7 vigun.IT97K-499-35.gnm1.ann1 Extra: 26857 18 17061 1581 1168.7 vigan.Gyeongwon.gnm3.ann1 Extra: 31241 150 16263 1518 1078.5 vigan.Shumari.gnm1.ann1 Extra: 22368 12 15450 1614 1264.3 vigra.VC1973A.gnm6.ann1 Extra: 30958 6 13857 1473 1081.7 vigra.VC1973A.gnm7.ann1 Avg: 28539 75 16061 1584 1209 all_annot_sets == Sequence stats for final pangene CDS files -- pctl25 and trimmed Class: seqs min max N50 ave annotation_name pctl25: 28687 84 23532 1617 1259.3 23_syn_pan_pctl25_posn_cds.fna == Proportion of initial genes retained in the "aug_extra" and "pctl25" sets: Start End_all End_core Pct_kept_all Pct_kept_core Annotation_name 26857 21247 21243 79.1 79.1 vigan.Gyeongwon.gnm3.ann1 31241 23146 23146 74.1 74.1 vigan.Shumari.gnm1.ann1 22368 19402 19401 86.7 86.7 vigra.VC1973A.gnm6.ann1 30958 24479 24475 79.1 79.1 vigra.VC1973A.gnm7.ann1 28297 27786 27445 98.2 97.0 vigun.CB5-2.gnm1.ann1 29773 28095 27937 94.4 93.8 vigun.IT97K-499-35.gnm1.ann1 31948 28669 28175 89.7 88.2 vigun.IT97K-499-35.gnm1.ann2 28461 26998 26663 94.9 93.7 vigun.Sanzi.gnm1.ann1 28545 27427 27074 96.1 94.8 vigun.Suvita2.gnm1.ann1 27742 27054 26698 97.5 96.2 vigun.TZ30.gnm1.ann2 28562 26979 26609 94.5 93.2 vigun.UCR779.gnm1.ann1 27723 26932 26586 97.1 95.9 vigun.ZN016.gnm1.ann2 == For all annotation sets, counts of genes-in-orthogroups and counts of orthogroups-with-genes: gns-in-OGs OGs-w-gns OGs-w-gns/gns pct-non-null-OGs pct-null-OGs annot-set 21247 19827 93.32 65.65 34.35 vigan.Gyeongwon.gnm3.ann1 23146 21675 93.64 71.77 28.23 vigan.Shumari.gnm1.ann1 19402 18200 93.80 60.26 39.74 vigra.VC1973A.gnm6.ann1 24479 21160 86.44 70.07 29.93 vigra.VC1973A.gnm7.ann1 27786 27088 97.49 89.70 10.30 vigun.CB5-2.gnm1.ann1 28095 26419 94.03 87.48 12.52 vigun.IT97K-499-35.gnm1.ann1 28669 27645 96.43 91.54 8.46 vigun.IT97K-499-35.gnm1.ann2 26998 26721 98.97 88.48 11.52 vigun.Sanzi.gnm1.ann1 27427 27147 98.98 89.89 10.11 vigun.Suvita2.gnm1.ann1 27054 26783 99.00 88.69 11.31 vigun.TZ30.gnm1.ann2 26979 26777 99.25 88.67 11.33 vigun.UCR779.gnm1.ann1 26932 26751 99.33 88.58 11.42 vigun.ZN016.gnm1.ann2 Counts of initial clusters by cluster size, file 06_syn_pan.clust.tsv: 2 1725 3 1059 4 926 5 1044 6 1627 7 22969 8 95 9 35 10 24 11 11 12 8 13 1 14 2 Counts of augmented clusters by cluster size, file 12_syn_pan_aug.clust.tsv: 2 1788 3 1241 4 1003 5 1030 6 1383 7 23402 8 193 9 63 10 34 11 26 12 14 13 7 14 7 15 1 16 3 17 1 22 1 24 2 28 1 Counts of augmented-extra clusters by cluster size, file 18_syn_pan_aug_extra.clust.tsv: 2 1000 3 999 4 815 5 798 6 746 7 936 8 1611 9 1309 10 2317 11 4719 12 12370 13 1364 14 603 15 238 16 162 17 83 18 41 19 17 20 18 21 9 22 7 23 4 24 5 25 5 26 5 27 2 28 2 30 1 31 1 32 1 33 1 34 1 35 2 36 1 44 1 48 1 53 1 55 1 57 1 64 1 76 1 # Number of annotations per pangene bin abcdefghijKLMNOPQRSTabcdefghijKLMNOPQRSTabcdefghijKLMNOPQRSTabcdefghijKLMNOPQRSTabcdefghijKLMNOPQRST 1.00 2.00 ....... 3.00 ....... 4.00 ..... 5.00 ..... 6.00 ..... 7.00 ...... 8.00 .......... 9.00 ......... 10.00 ................. 11.00 ................................... 12.00 .........................................................................................