##### PUB RECORD ##### ## 10.1111/nph.17097 33222243 Wang, Li et al., 2020 "Wang C, Ji W, Liu Y, Zhou P, Meng Y, Zhang P, Wen J, Mysore KS, Zhai J, Young ND, Tian Z, Niu L, Lin H. The antagonistic MYB paralogs RH1 and RH2 govern anthocyanin leaf markings in Medicago truncatula. New Phytol. 2021 Mar;229(6):3330-3344. doi: 10.1111/nph.17097. Epub 2020 Dec 23. PMID: 33222243; PMCID: PMC7986808." ## PMID- 33222243 OWN - NLM STAT- MEDLINE DCOM- 20210514 LR - 20210514 IS - 1469-8137 (Electronic) IS - 0028-646X (Print) IS - 0028-646X (Linking) VI - 229 IP - 6 DP - 2021 Mar TI - The antagonistic MYB paralogs RH1 and RH2 govern anthocyanin leaf markings in Medicago truncatula. PG - 3330-3344 LID - 10.1111/nph.17097 [doi] AB - Patterned leaf coloration in plants generates remarkable diversity in nature, but the underlying mechanisms remain largely unclear. Here, using Medicago truncatula leaf marking as a model, we show that the classic M. truncatula leaf anthocyanin spot trait depends on two R2R3 MYB paralogous regulators, RED HEART1 (RH1) and RH2. RH1 mainly functions as an anthocyanin biosynthesis activator that specifically determines leaf marking formation depending on its C-terminal activation motif. RH1 physically interacts with the M. truncatula bHLH protein MtTT8 and the WDR family member MtWD40-1, and this interaction facilitates RH1 function in leaf anthocyanin marking formation. RH2 has lost transcriptional activation activity, due to a divergent C-terminal domain, but retains the ability to interact with the same partners, MtTT8 and MtWD40-1, as RH1, thereby acting as a competitor in the regulatory complex and exerting opposite effects. Moreover, our results demonstrate that RH1 can activate its own expression and that RH2-mediated competition can repress RH1 expression. Our findings reveal the molecular mechanism of the antagonistic gene paralogs RH1 and RH2 in determining anthocyanin leaf markings in M. truncatula, providing a multidimensional paralogous-antagonistic regulatory paradigm for fine-tuning patterned pigmentation. CI - (c) 2020 The Authors New Phytologist (c) 2020 New Phytologist Foundation. FAU - Wang, Chongnan AU - Wang C AD - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. FAU - Ji, Wenkai AU - Ji W AD - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. FAU - Liu, Yucheng AU - Liu Y AD - State, Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, the Innovative Academy of Seed Design, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, 100101, China. FAU - Zhou, Peng AU - Zhou P AD - Department of Plant Pathology, University of Minnesota, St Paul, MN, 55108, USA. FAU - Meng, Yingying AU - Meng Y AD - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. FAU - Zhang, Pengcheng AU - Zhang P AD - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. FAU - Wen, Jiangqi AU - Wen J AUID- ORCID: 0000-0001-5113-7750 AD - Noble Research Institute, Ardmore, OK, 73401, USA. FAU - Mysore, Kirankumar S AU - Mysore KS AUID- ORCID: 0000-0002-9805-5741 AD - Noble Research Institute, Ardmore, OK, 73401, USA. FAU - Zhai, Jixian AU - Zhai J AD - Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, 518055, China. FAU - Young, Nevin D AU - Young ND AD - Department of Plant Pathology, University of Minnesota, St Paul, MN, 55108, USA. FAU - Tian, Zhixi AU - Tian Z AD - State, Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, the Innovative Academy of Seed Design, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, 100101, China. FAU - Niu, Lifang AU - Niu L AUID- ORCID: 0000-0001-5881-9033 AD - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. FAU - Lin, Hao AU - Lin H AUID- ORCID: 0000-0003-4717-8335 AD - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20201223 PL - England TA - New Phytol JT - The New phytologist JID - 9882884 RN - 0 (Anthocyanins) RN - 0 (Plant Proteins) RN - 0 (Transcription Factors) SB - IM MH - Anthocyanins MH - Gene Expression Regulation, Plant MH - *Medicago truncatula/genetics/metabolism MH - Plant Leaves/metabolism MH - Plant Proteins/genetics/metabolism MH - Plants, Genetically Modified/metabolism MH - Transcription Factors/genetics/metabolism PMC - PMC7986808 OTO - NOTNLM OT - MYB regulator OT - Medicago truncatula OT - anthocyanin leaf marking OT - paralog antagonism OT - patterned pigmentation EDAT- 2020/11/23 06:00 MHDA- 2021/05/15 06:00 PMCR- 2021/03/23 CRDT- 2020/11/22 20:46 PHST- 2020/10/20 00:00 [received] PHST- 2020/11/14 00:00 [accepted] PHST- 2020/11/23 06:00 [pubmed] PHST- 2021/05/15 06:00 [medline] PHST- 2020/11/22 20:46 [entrez] PHST- 2021/03/23 00:00 [pmc-release] AID - NPH17097 [pii] AID - 10.1111/nph.17097 [doi] PST - ppublish SO - New Phytol. 2021 Mar;229(6):3330-3344. doi: 10.1111/nph.17097. Epub 2020 Dec 23. ##### PUB RECORD ##### ## 10.1104/pp.18.01588 30782966 Laffont, Huault et al., 2019 "Laffont C, Huault E, Gautrat P, Endre G, Kalo P, Bourion V, Duc G, Frugier F. Independent Regulation of Symbiotic Nodulation by the SUNN Negative and CRA2 Positive Systemic Pathways. Plant Physiol. 2019 May;180(1):559-570. doi: 10.1104/pp.18.01588. Epub 2019 Feb 19. PMID: 30782966; PMCID: PMC6501087." ## PMID- 30782966 OWN - NLM STAT- MEDLINE DCOM- 20200226 LR - 20200501 IS - 1532-2548 (Electronic) IS - 0032-0889 (Print) IS - 0032-0889 (Linking) VI - 180 IP - 1 DP - 2019 May TI - Independent Regulation of Symbiotic Nodulation by the SUNN Negative and CRA2 Positive Systemic Pathways. PG - 559-570 LID - 10.1104/pp.18.01588 [doi] AB - Plant systemic signaling pathways allow the integration and coordination of shoot and root organ metabolism and development at the whole-plant level depending on nutrient availability. In legumes, two systemic pathways have been reported in the Medicago truncatula model to regulate root nitrogen-fixing symbiotic nodulation. Both pathways involve leucine-rich repeat receptor-like kinases acting in shoots and proposed to perceive signaling peptides produced in roots depending on soil nutrient availability. In this study, we characterized in the M. truncatula Jemalong A17 genotype a mutant allelic series affecting the Compact Root Architecture2 (CRA2) receptor. These analyses revealed that this pathway acts systemically from shoots to positively regulate nodulation and is required for the activity of carboxyl-terminally encoded peptides (CEPs). In addition, we generated a double mutant to test genetic interactions of the CRA2 systemic pathway with the CLAVATA3/EMBRYO SURROUNDING REGION peptide (CLE)/Super Numeric Nodule (SUNN) receptor systemic pathway negatively regulating nodule number from shoots, which revealed an intermediate nodule number phenotype close to the wild type. Finally, we showed that the nitrate inhibition of nodule numbers was observed in cra2 mutants but not in sunn and cra2 sunn mutants. Overall, these results suggest that CEP/CRA2 and CLE/SUNN systemic pathways act independently from shoots to regulate nodule numbers. CI - (c) 2019 American Society of Plant Biologists. All Rights Reserved. FAU - Laffont, Carole AU - Laffont C AUID- ORCID: 0000-0002-8447-1736 AD - Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Universite Paris Sud, Universite Paris Diderot, Institut National de la Recherche Agronomique, Universite d'Evry, Universite Paris-Saclay, 91190 Gif-sur-Yvette, France. FAU - Huault, Emeline AU - Huault E AD - Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Universite Paris Sud, Universite Paris Diderot, Institut National de la Recherche Agronomique, Universite d'Evry, Universite Paris-Saclay, 91190 Gif-sur-Yvette, France. FAU - Gautrat, Pierre AU - Gautrat P AD - Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Universite Paris Sud, Universite Paris Diderot, Institut National de la Recherche Agronomique, Universite d'Evry, Universite Paris-Saclay, 91190 Gif-sur-Yvette, France. FAU - Endre, Gabriella AU - Endre G AD - Institute of Plant Biology, Biological Research Centre, 6726 Szeged, Hungary. FAU - Kalo, Peter AU - Kalo P AUID- ORCID: 0000-0002-0404-8904 AD - National Agricultural and Innovation Center, Agricultural Biotechnology Institute, 2100 Godollo, Hungary. FAU - Bourion, Virginie AU - Bourion V AD - Agroecologie, Institut National de la Recherche Agronomique, AgroSup Dijon, Universite Bourgogne Franche-Comte, 21065 Dijon, France. FAU - Duc, Gerard AU - Duc G AUID- ORCID: 0000-0002-2921-3152 AD - Agroecologie, Institut National de la Recherche Agronomique, AgroSup Dijon, Universite Bourgogne Franche-Comte, 21065 Dijon, France. FAU - Frugier, Florian AU - Frugier F AUID- ORCID: 0000-0002-9783-7418 AD - Institute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Universite Paris Sud, Universite Paris Diderot, Institut National de la Recherche Agronomique, Universite d'Evry, Universite Paris-Saclay, 91190 Gif-sur-Yvette, France florian.frugier@cnrs.fr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190219 PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 RN - 0 (Plant Proteins) SB - IM MH - Medicago truncatula/*physiology MH - Metabolic Networks and Pathways MH - Mutation MH - Plant Proteins/genetics/*metabolism MH - Plant Root Nodulation/*physiology MH - Plant Roots/physiology MH - Symbiosis PMC - PMC6501087 EDAT- 2019/02/21 06:00 MHDA- 2020/02/27 06:00 PMCR- 2020/05/01 CRDT- 2019/02/21 06:00 PHST- 2019/01/03 00:00 [received] PHST- 2019/02/06 00:00 [accepted] PHST- 2019/02/21 06:00 [pubmed] PHST- 2020/02/27 06:00 [medline] PHST- 2019/02/21 06:00 [entrez] PHST- 2020/05/01 00:00 [pmc-release] AID - pp.18.01588 [pii] AID - 201801588DR1 [pii] AID - 10.1104/pp.18.01588 [doi] PST - ppublish SO - Plant Physiol. 2019 May;180(1):559-570. doi: 10.1104/pp.18.01588. Epub 2019 Feb 19. ##### PUB RECORD ##### ## 10.1111/nph.13634 26390061 Couzigou, Magne et al., 2015 "Couzigou JM, Magne K, Mondy S, Cosson V, Clements J, Ratet P. The legume NOOT-BOP-COCH-LIKE genes are conserved regulators of abscission, a major agronomical trait in cultivated crops. New Phytol. 2016 Jan;209(1):228-40. doi: 10.1111/nph.13634. Epub 2015 Sep 21. PMID: 26390061." ## PMID- 26390061 OWN - NLM STAT- MEDLINE DCOM- 20160927 LR - 20240109 IS - 1469-8137 (Electronic) IS - 0028-646X (Linking) VI - 209 IP - 1 DP - 2016 Jan TI - The legume NOOT-BOP-COCH-LIKE genes are conserved regulators of abscission, a major agronomical trait in cultivated crops. PG - 228-40 LID - 10.1111/nph.13634 [doi] AB - Plants are able to lose organs selectively through a process called abscission. This process relies on the differentiation of specialized territories at the junction between organs and the plant body that are called abscission zones (AZ). Several genes control the formation or functioning of these AZ. We have characterized BLADE-ON-PETIOLE (BOP) orthologues from several legume plants and studied their roles in the abscission process using a mutant approach. Here, we show that the Medicago truncatula NODULE ROOT (NOOT), the Pisum sativum COCHLEATA (COCH) and their orthologue in Lotus japonicus are strictly necessary for the abscission of not only petals, but also leaflets, leaves and fruits. We also showed that the expression pattern of the M. truncatula pNOOT::GUS fusion is associated with functional and vestigial AZs when expressed in Arabidopsis. In addition, we show that the stip mutant from Lupinus angustifolius, defective in stipule formation and leaf abscission, is mutated in a BOP orthologue. In conclusion, this study shows that this clade of proteins plays an important conserved role in promoting abscission of all aerial organs studied so far. CI - (c) 2015 CNRS New Phytologist (c) 2015 New Phytologist Trust. FAU - Couzigou, Jean-Malo AU - Couzigou JM AD - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Diderot, Universite Paris-Saclay, Batiment 630, 91405, Orsay, France. AD - Laboratoire de Recherche en Sciences Vegetales, UMR5546, Universite de Toulouse, CNRS, 31326, Castanet Tolosan, France. FAU - Magne, Kevin AU - Magne K AD - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Diderot, Universite Paris-Saclay, Batiment 630, 91405, Orsay, France. FAU - Mondy, Samuel AU - Mondy S AD - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Diderot, Universite Paris-Saclay, Batiment 630, 91405, Orsay, France. FAU - Cosson, Viviane AU - Cosson V AD - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Diderot, Universite Paris-Saclay, Batiment 630, 91405, Orsay, France. FAU - Clements, Jonathan AU - Clements J AD - Baron-Hay Court, South Perth, WA, 6151, Australia. FAU - Ratet, Pascal AU - Ratet P AD - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Diderot, Universite Paris-Saclay, Batiment 630, 91405, Orsay, France. LA - eng SI - GENBANK/AEM62768 SI - GENBANK/JN408495 SI - GENBANK/KC792647 SI - GENBANK/KP739832 SI - GENBANK/KP739833 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150921 PL - England TA - New Phytol JT - The New phytologist JID - 9882884 RN - 0 (Plant Proteins) SB - IM MH - Arabidopsis/genetics MH - Brassicaceae/genetics MH - Cluster Analysis MH - Crops, Agricultural MH - Fabaceae/*genetics/physiology MH - *Gene Expression Regulation, Plant MH - Lotus/genetics MH - Lupinus/genetics MH - Medicago truncatula/genetics/physiology MH - Multigene Family MH - Mutation MH - Pisum sativum/genetics MH - Plant Proteins/*genetics/metabolism OTO - NOTNLM OT - BLADE-ON-PETIOLE (BOP) OT - COCHLEATA (COCH) OT - Lotus japonicus OT - Medicago truncatula OT - NODULE ROOT (NOOT) OT - NOOT-BOP-COCH-LIKE (NBCL) OT - Pisum sativum OT - abscission EDAT- 2015/09/22 06:00 MHDA- 2016/09/28 06:00 CRDT- 2015/09/22 06:00 PHST- 2015/04/02 00:00 [received] PHST- 2015/08/04 00:00 [accepted] PHST- 2015/09/22 06:00 [entrez] PHST- 2015/09/22 06:00 [pubmed] PHST- 2016/09/28 06:00 [medline] AID - 10.1111/nph.13634 [doi] PST - ppublish SO - New Phytol. 2016 Jan;209(1):228-40. doi: 10.1111/nph.13634. Epub 2015 Sep 21. ##### PUB RECORD ##### ## 10.1111/nph.12198 23432463 Rey, Nars et al., 2013 "Rey T, Nars A, Bonhomme M, Bottin A, Huguet S, Balzergue S, Jardinaud MF, Bono JJ, Cullimore J, Dumas B, Gough C, Jacquet C. NFP, a LysM protein controlling Nod factor perception, also intervenes in Medicago truncatula resistance to pathogens. New Phytol. 2013 May;198(3):875-886. doi: 10.1111/nph.12198. Epub 2013 Feb 25. PMID: 23432463." ## PMID- 23432463 OWN - NLM STAT- MEDLINE DCOM- 20131125 LR - 20240109 IS - 1469-8137 (Electronic) IS - 0028-646X (Linking) VI - 198 IP - 3 DP - 2013 May TI - NFP, a LysM protein controlling Nod factor perception, also intervenes in Medicago truncatula resistance to pathogens. PG - 875-886 LID - 10.1111/nph.12198 [doi] AB - Plant LysM proteins control the perception of microbial-derived N-acetylglucosamine compounds for the establishment of symbiosis or activation of plant immunity. This raises questions about how plants, and notably legumes, can differentiate friends and foes using similar molecular actors and whether any receptors can intervene in both symbiosis and resistance. To study this question, nfp and lyk3 LysM-receptor like kinase mutants of Medicago truncatula that are affected in the early steps of nodulation, were analysed following inoculation with Aphanomyces euteiches, a root oomycete. The role of NFP in this interaction was further analysed by overexpression of NFP and by transcriptome analyses. nfp, but not lyk3, mutants were significantly more susceptible than wildtype plants to A. euteiches, whereas NFP overexpression increased resistance. Transcriptome analyses on A. euteiches inoculation showed that mutation in the NFP gene led to significant changes in the expression of c. 500 genes, notably involved in cell dynamic processes previously associated with resistance to pathogen penetration. nfp mutants also showed an increased susceptibility to the fungus Colletotrichum trifolii. These results demonstrate that NFP intervenes in M. truncatula immunity, suggesting an unsuspected role for NFP in the perception of pathogenic signals. CI - (c) 2013 The Authors. New Phytologist (c) 2013 New Phytologist Trust. FAU - Rey, Thomas AU - Rey T AD - Universite de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. AD - CNRS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. FAU - Nars, Amaury AU - Nars A AD - Universite de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. AD - CNRS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. FAU - Bonhomme, Maxime AU - Bonhomme M AD - Universite de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. AD - CNRS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. FAU - Bottin, Arnaud AU - Bottin A AD - Universite de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. AD - CNRS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. FAU - Huguet, Stephanie AU - Huguet S AD - Unite de Recherche en Genomique Vegetale (URGV), UMR INRA 1165, Universite d'Evry Val d'Essonne, ERL CNRS 8196, CP 5708, F-91057, Evry Cedex, France. FAU - Balzergue, Sandrine AU - Balzergue S AD - Unite de Recherche en Genomique Vegetale (URGV), UMR INRA 1165, Universite d'Evry Val d'Essonne, ERL CNRS 8196, CP 5708, F-91057, Evry Cedex, France. FAU - Jardinaud, Marie-Francoise AU - Jardinaud MF AD - INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France. FAU - Bono, Jean-Jacques AU - Bono JJ AD - INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France. AD - CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France. FAU - Cullimore, Julie AU - Cullimore J AD - INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France. AD - CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France. FAU - Dumas, Bernard AU - Dumas B AD - Universite de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. AD - CNRS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. FAU - Gough, Clare AU - Gough C AD - INRA, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR441, F-31326, Castanet-Tolosan, France. AD - CNRS, Laboratoire des Interactions Plantes-Microorganismes (LIPM), UMR2594, F-31326, Castanet-Tolosan, France. FAU - Jacquet, Christophe AU - Jacquet C AD - Universite de Toulouse, UPS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. AD - CNRS, UMR 5546, Laboratoire de Recherche en Sciences Vegetales, BP42617, Auzeville, F-31326, Castanet-Tolosan, France. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130225 PL - England TA - New Phytol JT - The New phytologist JID - 9882884 RN - 0 (Plant Proteins) RN - EC 2.7.11.1 (Protein Serine-Threonine Kinases) SB - IM MH - Aphanomyces/pathogenicity/physiology MH - Colletotrichum/*pathogenicity MH - Disease Resistance/genetics MH - Gene Expression Profiling MH - Gene Expression Regulation, Plant MH - *Host-Pathogen Interactions MH - Medicago truncatula/genetics/*metabolism/*microbiology MH - Mutation MH - Plant Diseases/genetics/microbiology MH - Plant Proteins/genetics/*metabolism MH - Plant Roots/metabolism/microbiology MH - Plants, Genetically Modified MH - Protein Serine-Threonine Kinases/genetics/metabolism MH - Symbiosis/physiology EDAT- 2013/02/26 06:00 MHDA- 2013/12/16 06:00 CRDT- 2013/02/26 06:00 PHST- 2012/11/21 00:00 [received] PHST- 2013/01/17 00:00 [accepted] PHST- 2013/02/26 06:00 [entrez] PHST- 2013/02/26 06:00 [pubmed] PHST- 2013/12/16 06:00 [medline] AID - 10.1111/nph.12198 [doi] PST - ppublish SO - New Phytol. 2013 May;198(3):875-886. doi: 10.1111/nph.12198. Epub 2013 Feb 25. ##### PUB RECORD ##### ## 10.1093/jxb/erac112 35294003 Wang, Lu et al., 2022 "Wang R, Lu N, Liu C, Dixon RA, Wu Q, Mao Y, Yang Y, Zheng X, He L, Zhao B, Zhang F, Yang S, Chen H, Jun JH, Li Y, Liu C, Liu Y, Chen J. MtGSTF7, a TT19-like GST gene, is essential for accumulation of anthocyanins, but not proanthocyanins in Medicago truncatula. J Exp Bot. 2022 Jun 24;73(12):4129-4146. doi: 10.1093/jxb/erac112. PMID: 35294003; PMCID: PMC9232208." ## PMID- 35294003 OWN - NLM STAT- MEDLINE DCOM- 20220628 LR - 20220803 IS - 1460-2431 (Electronic) IS - 0022-0957 (Print) IS - 0022-0957 (Linking) VI - 73 IP - 12 DP - 2022 Jun 24 TI - MtGSTF7, a TT19-like GST gene, is essential for accumulation of anthocyanins, but not proanthocyanins in Medicago truncatula. PG - 4129-4146 LID - 10.1093/jxb/erac112 [doi] AB - Anthocyanins and proanthocyanins (PAs) are two end products of the flavonoid biosynthesis pathway. They are believed to be synthesized in the endoplasmic reticulum and then sequestered into the vacuole. In Arabidopsis thaliana, TRANSPARENT TESTA 19 (TT19) is necessary for both anthocyanin and PA accumulation. Here, we found that MtGSTF7, a homolog of AtTT19, is essential for anthocyanin accumulation but not required for PA accumulation in Medicago truncatula. MtGSTF7 was induced by the anthocyanin regulator LEGUME ANTHOCYANIN PRODUCTION 1 (LAP1), and its tissue expression pattern correlated with anthocyanin deposition in M. truncatula. Tnt1-insertional mutants of MtGSTF7 lost anthocyanin accumulation in vegetative organs, and introducing a genomic fragment of MtGSTF7 could complement the mutant phenotypes. Additionally, the accumulation of anthocyanins induced by LAP1 was significantly reduced in mtgstf7 mutants. Yeast-one-hybridization and dual-luciferase reporter assays revealed that LAP1 could bind to the MtGSTF7 promoter to activate its expression. Ectopic expression of MtGSTF7 in tt19 mutants could rescue their anthocyanin deficiency, but not their PA defect. Furthermore, PA accumulation was not affected in the mtgstf7 mutants. Taken together, our results show that the mechanism of anthocyanin and PA accumulation in M. truncatula is different from that in A. thaliana, and provide a new target gene for engineering anthocyanins in plants. CI - (c) The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. FAU - Wang, Ruoruo AU - Wang R AUID- ORCID: 0000-0002-6294-9655 AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. AD - Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan 650500, China. FAU - Lu, Nan AU - Lu N AD - BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA. FAU - Liu, Chenggang AU - Liu C AD - BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA. FAU - Dixon, Richard A AU - Dixon RA AUID- ORCID: 0000-0001-8393-9408 AD - BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA. FAU - Wu, Qing AU - Wu Q AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Mao, Yawen AU - Mao Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Yang, Yating AU - Yang Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. AD - School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, China. FAU - Zheng, Xiaoling AU - Zheng X AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - He, Liangliang AU - He L AUID- ORCID: 0000-0002-2169-4326 AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. FAU - Zhao, Baolin AU - Zhao B AUID- ORCID: 0000-0001-8082-4461 AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. FAU - Zhang, Fan AU - Zhang F AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. FAU - Yang, Shengchao AU - Yang S AD - National and Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, Yunnan 650201, China. FAU - Chen, Haitao AU - Chen H AD - Sanjie Institute of Forage, Yangling, Shaanxi 712100, China. FAU - Jun, Ji Hyung AU - Jun JH AD - BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA. FAU - Li, Ying AU - Li Y AD - BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA. FAU - Liu, Changning AU - Liu C AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. FAU - Liu, Yu AU - Liu Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. FAU - Chen, Jianghua AU - Chen J AUID- ORCID: 0000-0003-0715-1859 AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. AD - Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan 650500, China. AD - School of Life Science, University of Science and Technology of China, Hefei, Anhui 230026, China. LA - eng GR - XDA26030301/Strategic Priority Research Program of the Chinese Academy of Sciences/ GR - U1702234/National Natural Science Foundation of China/ GR - 2015HA032/High-end Scientific and Technological Talents in Yunnan Province/ GR - 202101AW070004/Yunnan Fundamental Research Projects/ GR - 2021395/Youth Innovation Promotion Association CAS/ GR - 703285/National Science Foundation/ PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Exp Bot JT - Journal of experimental botany JID - 9882906 RN - 0 (Anthocyanins) RN - 0 (Plant Proteins) SB - IM MH - Anthocyanins/metabolism MH - *Arabidopsis/genetics/metabolism MH - Gene Expression Regulation, Plant MH - *Medicago truncatula/genetics/metabolism MH - Plant Proteins/genetics/metabolism PMC - PMC9232208 OTO - NOTNLM OT - Medicago truncatula OT - Anthocyanin OT - LAP1 OT - MtGSTF7 OT - glutathione-S-transferase OT - proanthocyanidin EDAT- 2022/03/17 06:00 MHDA- 2022/06/29 06:00 PMCR- 2022/03/16 CRDT- 2022/03/16 12:13 PHST- 2021/10/10 00:00 [received] PHST- 2022/03/12 00:00 [accepted] PHST- 2022/03/17 06:00 [pubmed] PHST- 2022/06/29 06:00 [medline] PHST- 2022/03/16 12:13 [entrez] PHST- 2022/03/16 00:00 [pmc-release] AID - 6549631 [pii] AID - erac112 [pii] AID - 10.1093/jxb/erac112 [doi] PST - ppublish SO - J Exp Bot. 2022 Jun 24;73(12):4129-4146. doi: 10.1093/jxb/erac112. ##### PUB RECORD ##### ## 10.1105/tpc.105.035394 16199614 Ivashuta, Liu et al., 2005 "Ivashuta S, Liu J, Liu J, Lohar DP, Haridas S, Bucciarelli B, VandenBosch KA, Vance CP, Harrison MJ, Gantt JS. RNA interference identifies a calcium-dependent protein kinase involved in Medicago truncatula root development. Plant Cell. 2005 Nov;17(11):2911-21. doi: 10.1105/tpc.105.035394. Epub 2005 Sep 30. PMID: 16199614; PMCID: PMC1276019." ## PMID- 16199614 OWN - NLM STAT- MEDLINE DCOM- 20060526 LR - 20241123 IS - 1040-4651 (Print) IS - 1532-298X (Electronic) IS - 1040-4651 (Linking) VI - 17 IP - 11 DP - 2005 Nov TI - RNA interference identifies a calcium-dependent protein kinase involved in Medicago truncatula root development. PG - 2911-21 AB - Changes in cellular or subcellular Ca2+ concentrations play essential roles in plant development and in the responses of plants to their environment. However, the mechanisms through which Ca2+ acts, the downstream signaling components, as well as the relationships among the various Ca2+-dependent processes remain largely unknown. Using an RNA interference-based screen for gene function in Medicago truncatula, we identified a gene that is involved in root development. Silencing Ca2+-dependent protein kinase1 (CDPK1), which is predicted to encode a Ca2+-dependent protein kinase, resulted in significantly reduced root hair and root cell lengths. Inactivation of CDPK1 is also associated with significant diminution of both rhizobial and mycorrhizal symbiotic colonization. Additionally, microarray analysis revealed that silencing CDPK1 alters cell wall and defense-related gene expression. We propose that M. truncatula CDPK1 is a key component of one or more signaling pathways that directly or indirectly modulates cell expansion or cell wall synthesis, possibly altering defense gene expression and symbiotic interactions. FAU - Ivashuta, Sergey AU - Ivashuta S AD - Department of Plant Biology, University of Minesota, St. Paul, Minesota 55108, USA. FAU - Liu, Jinyuan AU - Liu J FAU - Liu, Junqi AU - Liu J FAU - Lohar, Dasharath P AU - Lohar DP FAU - Haridas, Sajeet AU - Haridas S FAU - Bucciarelli, Bruna AU - Bucciarelli B FAU - VandenBosch, Kathryn A AU - VandenBosch KA FAU - Vance, Carroll P AU - Vance CP FAU - Harrison, Maria J AU - Harrison MJ FAU - Gantt, J Stephen AU - Gantt JS LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20050930 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 RN - 0 (ATCDPK1 protein, Arabidopsis) RN - 0 (Arabidopsis Proteins) RN - 0 (Calcium-Binding Proteins) RN - EC 2.7.- (Protein Kinases) RN - SY7Q814VUP (Calcium) SB - IM MH - Arabidopsis Proteins/genetics/metabolism MH - Calcium/metabolism MH - Calcium Signaling/physiology MH - Calcium-Binding Proteins/genetics/isolation & purification/*metabolism MH - Cell Wall/enzymology/genetics MH - Gene Expression Regulation, Enzymologic/physiology MH - Gene Expression Regulation, Plant/genetics MH - Gene Silencing/physiology MH - Immunity, Innate/genetics MH - Medicago truncatula/*enzymology/genetics/*growth & development MH - Plant Diseases/genetics MH - Plant Roots/*enzymology/genetics/*growth & development MH - Protein Kinases/genetics/isolation & purification/*metabolism MH - RNA Interference/physiology MH - Signal Transduction/genetics MH - Symbiosis/genetics PMC - PMC1276019 EDAT- 2005/10/04 09:00 MHDA- 2006/05/27 09:00 PMCR- 2006/11/01 CRDT- 2005/10/04 09:00 PHST- 2005/10/04 09:00 [pubmed] PHST- 2006/05/27 09:00 [medline] PHST- 2005/10/04 09:00 [entrez] PHST- 2006/11/01 00:00 [pmc-release] AID - tpc.105.035394 [pii] AID - 035394 [pii] AID - 10.1105/tpc.105.035394 [doi] PST - ppublish SO - Plant Cell. 2005 Nov;17(11):2911-21. doi: 10.1105/tpc.105.035394. Epub 2005 Sep 30. ##### PUB RECORD ##### ## 10.1104/pp.111.180182 21685176 Laurie, Diwadkar et al., 2011 "Laurie RE, Diwadkar P, Jaudal M, Zhang L, Hecht V, Wen J, Tadege M, Mysore KS, Putterill J, Weller JL, Macknight RC. The Medicago FLOWERING LOCUS T homolog, MtFTa1, is a key regulator of flowering time. Plant Physiol. 2011 Aug;156(4):2207-24. doi: 10.1104/pp.111.180182. Epub 2011 Jun 17. PMID: 21685176; PMCID: PMC3149922." ## PMID- 21685176 OWN - NLM STAT- MEDLINE DCOM- 20111205 LR - 20240109 IS - 1532-2548 (Electronic) IS - 0032-0889 (Print) IS - 0032-0889 (Linking) VI - 156 IP - 4 DP - 2011 Aug TI - The Medicago FLOWERING LOCUS T homolog, MtFTa1, is a key regulator of flowering time. PG - 2207-24 LID - 10.1104/pp.111.180182 [doi] AB - FLOWERING LOCUS T (FT) genes encode proteins that function as the mobile floral signal, florigen. In this study, we characterized five FT-like genes from the model legume, Medicago (Medicago truncatula). The different FT genes showed distinct patterns of expression and responses to environmental cues. Three of the FT genes (MtFTa1, MtFTb1, and MtFTc) were able to complement the Arabidopsis (Arabidopsis thaliana) ft-1 mutant, suggesting that they are capable of functioning as florigen. MtFTa1 is the only one of the FT genes that is up-regulated by both long days (LDs) and vernalization, conditions that promote Medicago flowering, and transgenic Medicago plants overexpressing the MtFTa1 gene flowered very rapidly. The key role MtFTa1 plays in regulating flowering was demonstrated by the identification of fta1 mutants that flowered significantly later in all conditions examined. fta1 mutants do not respond to vernalization but are still responsive to LDs, indicating that the induction of flowering by prolonged cold acts solely through MtFTa1, whereas photoperiodic induction of flowering involves other genes, possibly MtFTb1, which is only expressed in leaves under LD conditions and therefore might contribute to the photoperiodic regulation of flowering. The role of the MtFTc gene is unclear, as the ftc mutants did not have any obvious flowering-time or other phenotypes. Overall, this work reveals the diversity of the regulation and function of the Medicago FT family. FAU - Laurie, Rebecca E AU - Laurie RE AD - Department of Biochemistry, University of Otago, Dunedin 9054, New Zealand. FAU - Diwadkar, Payal AU - Diwadkar P FAU - Jaudal, Mauren AU - Jaudal M FAU - Zhang, Lulu AU - Zhang L FAU - Hecht, Valerie AU - Hecht V FAU - Wen, Jiangqi AU - Wen J FAU - Tadege, Million AU - Tadege M FAU - Mysore, Kirankumar S AU - Mysore KS FAU - Putterill, Joanna AU - Putterill J FAU - Weller, James L AU - Weller JL FAU - Macknight, Richard C AU - Macknight RC LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20110617 PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 RN - 0 (Plant Proteins) SB - IM MH - Amino Acid Sequence MH - Arabidopsis/genetics MH - Cold Temperature MH - Flowers/genetics/growth & development/*physiology MH - Gene Expression Regulation, Plant MH - Genes, Plant/genetics MH - Genetic Complementation Test MH - Medicago/genetics/growth & development/*physiology MH - Meristem/genetics MH - Molecular Sequence Data MH - Mutation/genetics MH - Phenotype MH - Photoperiod MH - Plant Proteins/chemistry/genetics/*metabolism MH - Plants, Genetically Modified MH - *Sequence Homology, Amino Acid MH - Time Factors MH - Up-Regulation/genetics PMC - PMC3149922 EDAT- 2011/06/21 06:00 MHDA- 2011/12/13 00:00 PMCR- 2011/06/17 CRDT- 2011/06/21 06:00 PHST- 2011/06/21 06:00 [entrez] PHST- 2011/06/21 06:00 [pubmed] PHST- 2011/12/13 00:00 [medline] PHST- 2011/06/17 00:00 [pmc-release] AID - pp.111.180182 [pii] AID - 180182 [pii] AID - 10.1104/pp.111.180182 [doi] PST - ppublish SO - Plant Physiol. 2011 Aug;156(4):2207-24. doi: 10.1104/pp.111.180182. Epub 2011 Jun 17. ##### PUB RECORD ##### ## 10.1186/s12870-019-2194-z 31856724 Shen, Kulikova et al., 2019 "Shen D, Kulikova O, Guhl K, Franssen H, Kohlen W, Bisseling T, Geurts R. The Medicago truncatula nodule identity gene MtNOOT1 is required for coordinated apical-basal development of the root. BMC Plant Biol. 2019 Dec 19;19(1):571. doi: 10.1186/s12870-019-2194-z. PMID: 31856724; PMCID: PMC6923920." ## PMID- 31856724 OWN - NLM STAT- MEDLINE DCOM- 20200403 LR - 20200403 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 19 IP - 1 DP - 2019 Dec 19 TI - The Medicago truncatula nodule identity gene MtNOOT1 is required for coordinated apical-basal development of the root. PG - 571 LID - 10.1186/s12870-019-2194-z [doi] LID - 571 AB - BACKGROUND: Legumes can utilize atmospheric nitrogen by hosting nitrogen-fixing bacteria in special lateral root organs, called nodules. Legume nodules have a unique ontology, despite similarities in the gene networks controlling nodule and lateral root development. It has been shown that Medicago truncatula NODULE ROOT1 (MtNOOT1) is required for the maintenance of nodule identity, preventing the conversion to lateral root development. MtNOOT1 and its orthologs in other plant species -collectively called the NOOT-BOP-COCH-LIKE (NBCL) family- specify boundary formation in various aerial organs. However, MtNOOT1 is not only expressed in nodules and aerial organs, but also in developing roots, where its function remains elusive. RESULTS: We show that Mtnoot1 mutant seedlings display accelerated root elongation due to an enlarged root apical meristem. Also, Mtnoot1 mutant roots are thinner than wild-type and are delayed in xylem cell differentiation. We provide molecular evidence that the affected spatial development of Mtnoot1 mutant roots correlates with delayed induction of genes involved in xylem cell differentiation. This coincides with a basipetal shift of the root zone that is susceptible to rhizobium-secreted symbiotic signal molecules. CONCLUSIONS: Our data show that MtNOOT1 regulates the size of the root apical meristem and vascular differentiation. Our data demonstrate that MtNOOT1 not only functions as a homeotic gene in nodule development but also coordinates the spatial development of the root. FAU - Shen, Defeng AU - Shen D AD - Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands. FAU - Kulikova, Olga AU - Kulikova O AD - Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands. FAU - Guhl, Kerstin AU - Guhl K AD - Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands. FAU - Franssen, Henk AU - Franssen H AD - Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands. FAU - Kohlen, Wouter AU - Kohlen W AD - Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands. FAU - Bisseling, Ton AU - Bisseling T AD - Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands. FAU - Geurts, Rene AU - Geurts R AD - Department of Plant Science, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands. rene.geurts@wur.nl. LA - eng GR - 201306040120/China Scholarship Council/ GR - ERC-2011-AdG294790/ERC_/European Research Council/International GR - 865.13.001/The Netherlands Organisation for Scientific Research/ PT - Journal Article DEP - 20191219 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 RN - 0 (Plant Proteins) SB - IM MH - Medicago truncatula/*genetics/growth & development MH - Organogenesis, Plant/*genetics MH - Plant Proteins/*genetics/metabolism MH - Root Nodules, Plant/genetics/*growth & development PMC - PMC6923920 OTO - NOTNLM OT - Medicago truncatula OT - NBCL OT - NIN OT - NOOT-BOP-COCHLEATA-LIKE OT - NOOT1 OT - Rhizobium susceptible zone OT - Xylem cell differentiation COIS- The authors declare that they have no competing interests. RG is a member of the editorial board (Associate Editor) of BMC Plant Biology. EDAT- 2019/12/21 06:00 MHDA- 2020/04/04 06:00 PMCR- 2019/12/19 CRDT- 2019/12/21 06:00 PHST- 2019/09/21 00:00 [received] PHST- 2019/12/10 00:00 [accepted] PHST- 2019/12/21 06:00 [entrez] PHST- 2019/12/21 06:00 [pubmed] PHST- 2020/04/04 06:00 [medline] PHST- 2019/12/19 00:00 [pmc-release] AID - 10.1186/s12870-019-2194-z [pii] AID - 2194 [pii] AID - 10.1186/s12870-019-2194-z [doi] PST - epublish SO - BMC Plant Biol. 2019 Dec 19;19(1):571. doi: 10.1186/s12870-019-2194-z. ##### PUB RECORD ##### ## 10.1111/nph.13162 25406544 Carelli, Biazzi et al., 2014 "Carelli M, Biazzi E, Tava A, Losini I, Abbruscato P, Depedro C, Scotti C. Sapogenin content variation in Medicago inter-specific hybrid derivatives highlights some aspects of saponin synthesis and control. New Phytol. 2015 Apr;206(1):303-314. doi: 10.1111/nph.13162. Epub 2014 Nov 18. PMID: 25406544." ## PMID- 25406544 OWN - NLM STAT- MEDLINE DCOM- 20160211 LR - 20201109 IS - 1469-8137 (Electronic) IS - 0028-646X (Linking) VI - 206 IP - 1 DP - 2015 Apr TI - Sapogenin content variation in Medicago inter-specific hybrid derivatives highlights some aspects of saponin synthesis and control. PG - 303-314 LID - 10.1111/nph.13162 [doi] AB - In the Medicago genus, saponins are a complex mixture of triterpene glycosides showing a broad spectrum of biological properties. Here we analyzed the variation in the sapogenin content and composition of inter-specific hybrid Medicago sativa x Medicago arborea derivatives to highlight the pattern of this variation in plant organs (leaves/roots) and the possible mechanisms underlying it. In Sativa Arborea Cross (SAC) leaves and roots, saponins and sapogenins were evaluated using chromatographic methods. Phenotypic correlations between sapogenin content and bio-agronomic traits were examined. Expression studies on beta-amyrin synthase and four cytochromes P450 (CYPs) involved in sapogenin biosynthesis and sequence analysis of the key gene of the hemolytic sapogenin pathway (CYP716A12) were performed. Chromatographic analyses revealed a different pattern of among-family variation for hemolytic and nonhemolytic sapogenins and saponins and for the two organs/tissues. Different correlation patterns of gene expression in roots and leaves were found. Diachronic analysis revealed a relationship between sapogenin content and gene transcriptional levels in the early stages of the productive cycle. The results suggest that there are different control mechanisms acting on sapogenin biosynthesis for leaves and roots, which are discussed. A key role for medicagenic acid in the control of sapogenin content in both the tissues is proposed and discussed. CI - (c) 2014 The Authors. New Phytologist (c) 2014 New Phytologist Trust. FAU - Carelli, Maria AU - Carelli M AD - Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 26900, Lodi, Italy. FAU - Biazzi, Elisa AU - Biazzi E AD - Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 26900, Lodi, Italy. FAU - Tava, Aldo AU - Tava A AD - Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 26900, Lodi, Italy. FAU - Losini, Ilaria AU - Losini I AD - Parco Tecnologico Padano, via Einsten- Loc. Cascina Codazza, 26900, Lodi, Italy. FAU - Abbruscato, Pamela AU - Abbruscato P AD - Parco Tecnologico Padano, via Einsten- Loc. Cascina Codazza, 26900, Lodi, Italy. FAU - Depedro, Claudia AU - Depedro C AD - Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 26900, Lodi, Italy. FAU - Scotti, Carla AU - Scotti C AD - Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, viale Piacenza 29, 26900, Lodi, Italy. LA - eng SI - GENBANK/KM978958 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141118 PL - England TA - New Phytol JT - The New phytologist JID - 9882884 RN - 0 (Plant Proteins) RN - 0 (Sapogenins) RN - 0 (Saponins) RN - 0 (Triterpenes) RN - 7X05537I17 (medicagenic acid) RN - 9035-51-2 (Cytochrome P-450 Enzyme System) RN - EC 5.4.- (Intramolecular Transferases) RN - EC 5.4.99.- (2,3-oxidosqualene-beta-amyrin-cyclase) SB - IM MH - Base Sequence MH - Cytochrome P-450 Enzyme System/*genetics/metabolism MH - Gene Expression Regulation, Plant MH - Intramolecular Transferases/*genetics/metabolism MH - Medicago/genetics/*metabolism MH - Medicago sativa/genetics/metabolism MH - Medicago truncatula/genetics/metabolism MH - Molecular Sequence Data MH - Organ Specificity MH - Plant Leaves/genetics/metabolism MH - Plant Proteins/genetics/metabolism MH - Plant Roots/genetics/metabolism MH - Sapogenins/*metabolism MH - Saponins/*metabolism MH - Sequence Analysis, DNA MH - Triterpenes/metabolism OTO - NOTNLM OT - Medicago arborea OT - Medicago sativa (alfalfa) OT - cytochrome P450 expression OT - inter-specific cross OT - sapogenin synthesis OT - triterpene saponin EDAT- 2014/11/20 06:00 MHDA- 2016/02/13 06:00 CRDT- 2014/11/20 06:00 PHST- 2014/08/08 00:00 [received] PHST- 2014/10/09 00:00 [accepted] PHST- 2014/11/20 06:00 [entrez] PHST- 2014/11/20 06:00 [pubmed] PHST- 2016/02/13 06:00 [medline] AID - 10.1111/nph.13162 [doi] PST - ppublish SO - New Phytol. 2015 Apr;206(1):303-314. doi: 10.1111/nph.13162. Epub 2014 Nov 18. ##### PUB RECORD ##### ## 10.1105/tpc.107.057547 18381924 Muller, Bleckmann et al., 2008 "Müller R, Bleckmann A, Simon R. The receptor kinase CORYNE of Arabidopsis transmits the stem cell-limiting signal CLAVATA3 independently of CLAVATA1. Plant Cell. 2008 Apr;20(4):934-46. doi: 10.1105/tpc.107.057547. Epub 2008 Apr 1. PMID: 18381924; PMCID: PMC2390746." ## PMID- 18381924 OWN - NLM STAT- MEDLINE DCOM- 20080805 LR - 20220408 IS - 1040-4651 (Print) IS - 1532-298X (Electronic) IS - 1040-4651 (Linking) VI - 20 IP - 4 DP - 2008 Apr TI - The receptor kinase CORYNE of Arabidopsis transmits the stem cell-limiting signal CLAVATA3 independently of CLAVATA1. PG - 934-46 LID - 10.1105/tpc.107.057547 [doi] AB - Stem cells in shoot and floral meristems of Arabidopsis thaliana secrete the signaling peptide CLAVATA3 (CLV3) that restricts stem cell proliferation and promotes differentiation. The CLV3 signaling pathway is proposed to comprise the receptor kinase CLV1 and the receptor-like protein CLV2. We show here that the novel receptor kinase CORYNE (CRN) and CLV2 act together, and in parallel with CLV1, to perceive the CLV3 signal. Mutations in CRN cause stem cell proliferation, similar to clv1, clv2, and clv3 mutants. CRN has additional functions during plant development, including floral organ development, that are shared with CLV2. The CRN protein lacks a distinct extracellular domain, and we propose that CRN and CLV2 interact via their transmembrane domains to establish a functional receptor. FAU - Muller, Ralf AU - Muller R AD - Institut fur Genetik, Heinrich-Heine University, D-40225 Dusseldorf, Germany. FAU - Bleckmann, Andrea AU - Bleckmann A FAU - Simon, Rudiger AU - Simon R LA - eng SI - RefSeq/NM_121332 SI - RefSeq/NM_180481 SI - RefSeq/NP_973541 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080401 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 RN - 0 (AT2G27250 protein, Arabidopsis) RN - 0 (Arabidopsis Proteins) RN - 0 (DNA Primers) RN - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) RN - EC 2.7.11.1 (CLV1 protein, Arabidopsis) RN - EC 2.7.11.1 (Protein Serine-Threonine Kinases) SB - IM MH - Amino Acid Sequence MH - Arabidopsis/*enzymology MH - Arabidopsis Proteins/*metabolism MH - Base Sequence MH - DNA Primers MH - In Situ Hybridization MH - Meristem/growth & development MH - Molecular Sequence Data MH - Plants, Genetically Modified MH - Protein Serine-Threonine Kinases MH - Receptor Protein-Tyrosine Kinases/chemistry/*metabolism MH - Sequence Homology, Amino Acid MH - *Signal Transduction MH - Stem Cells/*metabolism PMC - PMC2390746 EDAT- 2008/04/03 09:00 MHDA- 2008/08/06 09:00 PMCR- 2009/04/01 CRDT- 2008/04/03 09:00 PHST- 2008/04/03 09:00 [pubmed] PHST- 2008/08/06 09:00 [medline] PHST- 2008/04/03 09:00 [entrez] PHST- 2009/04/01 00:00 [pmc-release] AID - tpc.107.057547 [pii] AID - 057547 [pii] AID - 10.1105/tpc.107.057547 [doi] PST - ppublish SO - Plant Cell. 2008 Apr;20(4):934-46. doi: 10.1105/tpc.107.057547. Epub 2008 Apr 1. ##### PUB RECORD ##### ## 10.1105/tpc.019406 15037734 Campalans, Kondorosi et al., 2017 "Campalans A, Kondorosi A, Crespi M. Enod40, a short open reading frame-containing mRNA, induces cytoplasmic localization of a nuclear RNA binding protein in Medicago truncatula. Plant Cell. 2004 Apr;16(4):1047-59. doi: 10.1105/tpc.019406. Epub 2004 Mar 22. Erratum in: Plant Cell. 2017 Apr;29(4):912. doi: 10.1105/tpc.17.00245. PMID: 15037734; PMCID: PMC412876." ## PMID- 15037734 OWN - NLM STAT- MEDLINE DCOM- 20040625 LR - 20181113 IS - 1040-4651 (Print) IS - 1532-298X (Electronic) IS - 1040-4651 (Linking) VI - 16 IP - 4 DP - 2004 Apr TI - Enod40, a short open reading frame-containing mRNA, induces cytoplasmic localization of a nuclear RNA binding protein in Medicago truncatula. PG - 1047-59 AB - In eukaryotes, diverse mRNAs containing only short open reading frames (sORF-mRNAs) are induced at specific stages of development. Their mechanisms of action may involve the RNA itself and/or sORF-encoded oligopeptides. Enod40 genes code for highly structured plant sORF-mRNAs involved in root nodule organogenesis. A novel RNA binding protein interacting with the enod40 RNA, MtRBP1 (for Medicago truncatula RNA Binding Protein 1), was identified using a yeast three-hybrid screening. Immunolocalization studies and use of a MtRBP1-DsRed2 fluorescent protein fusion showed that MtRBP1 localized to nuclear speckles in plant cells but was exported into the cytoplasm during nodule development in enod40-expressing cells. Direct involvement of the enod40 RNA in MtRBP1 relocalization into cytoplasmic granules was shown using a transient expression assay. Using a (green fluorescent protein)/MS2 bacteriophage system to tag the enod40 RNA, we detected in vivo colocalization of the enod40 RNA and MtRBP1 in these granules. This in vivo approach to monitor RNA-protein interactions allowed us to demonstrate that cytoplasmic relocalization of nuclear proteins is an RNA-mediated cellular function of a sORF-mRNA. FAU - Campalans, Anna AU - Campalans A AD - Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France. FAU - Kondorosi, Adam AU - Kondorosi A FAU - Crespi, Martin AU - Crespi M LA - eng SI - GENBANK/AJ508392 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20040322 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 RN - 0 (DNA, Plant) RN - 0 (DNA-Binding Proteins) RN - 0 (ENOD40 RNA) RN - 0 (Nuclear Proteins) RN - 0 (Plant Proteins) RN - 0 (RNA, Long Noncoding) RN - 0 (RNA, Messenger) RN - 0 (RNA, Plant) RN - 0 (RNA, Untranslated) RN - 0 (Recombinant Fusion Proteins) SB - IM EIN - Plant Cell. 2017 Apr;29(4):912. doi: 10.1105/tpc.17.00245. PMID: 28351988 MH - Amino Acid Sequence MH - Base Sequence MH - Cytoplasm/metabolism MH - DNA, Plant/genetics MH - DNA-Binding Proteins/genetics/metabolism MH - Gene Expression MH - Medicago/*genetics/*metabolism MH - Molecular Sequence Data MH - Nuclear Proteins/genetics/metabolism MH - Onions/genetics/metabolism MH - Plant Proteins/genetics/metabolism MH - Plants, Genetically Modified MH - RNA, Long Noncoding MH - RNA, Messenger/*genetics MH - RNA, Plant/*genetics MH - RNA, Untranslated/*genetics MH - Recombinant Fusion Proteins/genetics/metabolism MH - Sequence Homology, Amino Acid MH - Two-Hybrid System Techniques PMC - PMC412876 EDAT- 2004/03/24 05:00 MHDA- 2004/06/26 05:00 PMCR- 2005/04/01 CRDT- 2004/03/24 05:00 PHST- 2004/03/24 05:00 [pubmed] PHST- 2004/06/26 05:00 [medline] PHST- 2004/03/24 05:00 [entrez] PHST- 2005/04/01 00:00 [pmc-release] AID - tpc.019406 [pii] AID - 019406 [pii] AID - 10.1105/tpc.019406 [doi] PST - ppublish SO - Plant Cell. 2004 Apr;16(4):1047-59. doi: 10.1105/tpc.019406. Epub 2004 Mar 22. ##### PUB RECORD ##### ## 10.3390/ijms251910410 39408738 Xing, Weng etal., 2024 "Xing J, Wang J, Cao J, Li K, Meng X, Wen J, Mysore KS, Wang G, Zhou C, Yin P. Identification of a Novel Gene MtbZIP60 as a Negative Regulator of Leaf Senescence through Transcriptome Analysis in Medicago truncatula. Int J Mol Sci. 2024 Sep 27;25(19):10410. doi: 10.3390/ijms251910410. PMID: 39408738; PMCID: PMC11477300." ## PMID- 39408738 OWN - NLM STAT- MEDLINE DCOM- 20241016 LR - 20241023 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 25 IP - 19 DP - 2024 Sep 27 TI - Identification of a Novel Gene MtbZIP60 as a Negative Regulator of Leaf Senescence through Transcriptome Analysis in Medicago truncatula. LID - 10.3390/ijms251910410 [doi] LID - 10410 AB - Leaves are the primary harvest portion in forage crops such as alfalfa (Medicago sativa). Delaying leaf senescence is an effective strategy to improve forage biomass production and quality. In this study, we employed transcriptome sequencing to analyze the transcriptional changes and identify key senescence-associated genes under age-dependent leaf senescence in Medicago truncatula, a legume forage model plant. Through comparing the obtained expression data at different time points, we obtained 1057 differentially expressed genes, with 108 consistently up-regulated genes across leaf growth and senescence. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses showed that the 108 SAGs mainly related to protein processing, nitrogen metabolism, amino acid metabolism, RNA degradation and plant hormone signal transduction. Among the 108 SAGs, seven transcription factors were identified in which a novel bZIP transcription factor MtbZIP60 was proved to inhibit leaf senescence. MtbZIP60 encodes a nuclear-localized protein and possesses transactivation activity. Further study demonstrated MtbZIP60 could associate with MtWRKY40, both of which exhibited an up-regulated expression pattern during leaf senescence, indicating their crucial roles in the regulation of leaf senescence. Our findings help elucidate the molecular mechanisms of leaf senescence in M. truncatula and provide candidates for the genetic improvement of forage crops, with a focus on regulating leaf senescence. FAU - Xing, Jiayu AU - Xing J AD - Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China. FAU - Wang, Jialan AU - Wang J AD - Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China. FAU - Cao, Jianuo AU - Cao J AD - Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China. FAU - Li, Ke AU - Li K AD - Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China. FAU - Meng, Xiao AU - Meng X AD - Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China. FAU - Wen, Jiangqi AU - Wen J AUID- ORCID: 0000-0001-5113-7750 AD - Department of Plant and Soil Sciences, Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK 73401, USA. FAU - Mysore, Kirankumar S AU - Mysore KS AUID- ORCID: 0000-0002-9805-5741 AD - Department of Biochemistry and Molecular Biology, Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK 73401, USA. FAU - Wang, Geng AU - Wang G AD - Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China. FAU - Zhou, Chunjiang AU - Zhou C AUID- ORCID: 0000-0003-1654-3782 AD - Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China. FAU - Yin, Pengcheng AU - Yin P AD - Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China. LA - eng GR - C2022205038/Natural Science Foundation of Hebei Province/ GR - C2022205015/Hebei Province Outstanding Youth Fund/ GR - C2023205050/Natural Science Foundation of Hebei Province/ GR - HBCT2024030201/Modern Agro-industry Technology Research Team of Hebei/ GR - B2021003018/Postdoctoral Preferred Funding Research Project of Hebei Province/ PT - Journal Article DEP - 20240927 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Plant Proteins) RN - 0 (Transcription Factors) RN - 0 (Basic-Leucine Zipper Transcription Factors) SB - IM MH - *Medicago truncatula/genetics/growth & development/metabolism MH - *Plant Leaves/genetics/growth & development/metabolism MH - *Gene Expression Regulation, Plant MH - *Plant Proteins/genetics/metabolism MH - *Gene Expression Profiling/methods MH - Plant Senescence/genetics MH - Transcriptome/genetics MH - Transcription Factors/genetics/metabolism MH - Basic-Leucine Zipper Transcription Factors/genetics/metabolism PMC - PMC11477300 OTO - NOTNLM OT - Medicago truncatula OT - WRKY OT - bZIP OT - leaf senescence OT - transcriptome analysis COIS- The authors declare no conflicts of interest. EDAT- 2024/10/16 10:18 MHDA- 2024/10/16 10:19 PMCR- 2024/09/27 CRDT- 2024/10/16 01:08 PHST- 2024/09/03 00:00 [received] PHST- 2024/09/24 00:00 [revised] PHST- 2024/09/25 00:00 [accepted] PHST- 2024/10/16 10:19 [medline] PHST- 2024/10/16 10:18 [pubmed] PHST- 2024/10/16 01:08 [entrez] PHST- 2024/09/27 00:00 [pmc-release] AID - ijms251910410 [pii] AID - ijms-25-10410 [pii] AID - 10.3390/ijms251910410 [doi] PST - epublish SO - Int J Mol Sci. 2024 Sep 27;25(19):10410. doi: 10.3390/ijms251910410. ##### PUB RECORD ##### ## 10.1094/MPMI.2000.13.7.763 10875337 Salzer, Bonanomi et al., 2000 "Salzer P, Bonanomi A, Beyer K, Vögeli-Lange R, Aeschbacher RA, Lange J, Wiemken A, Kim D, Cook DR, Boller T. Differential expression of eight chitinase genes in Medicago truncatula roots during mycorrhiza formation, nodulation, and pathogen infection. Mol Plant Microbe Interact. 2000 Jul;13(7):763-77. doi: 10.1094/MPMI.2000.13.7.763. PMID: 10875337." ## PMID- 10875337 OWN - NLM STAT- MEDLINE DCOM- 20001026 LR - 20240109 IS - 0894-0282 (Print) IS - 0894-0282 (Linking) VI - 13 IP - 7 DP - 2000 Jul TI - Differential expression of eight chitinase genes in Medicago truncatula roots during mycorrhiza formation, nodulation, and pathogen infection. PG - 763-77 AB - Expression of eight different chitinase genes, representing members of five chitinase classes, was studied in Medicago truncatula roots during formation of arbuscular mycorrhiza with Glomus intraradices, nodulation with Rhizobium meliloti, and pathogen attack by Phytophthora megasperma f. sp. medicaginis, Fusarium solani f. sp. phaseoli (compatible interactions with root rot symptoms), Ascochyta pisi (compatible, symptomless), and F. solani f. sp. pisi (incompatible, nonhost interaction). In the compatible plant-pathogen interactions, expression of class I, II, and IV chitinase genes was enhanced. The same genes were induced during nodulation. Transcripts of class I and II chitinase genes accumulated transiently during early stages of the interaction, and transcripts of the class IV chitinase gene accumulated in mature nodules. The pattern of chitinase gene expression in mycorrhizal roots was markedly different: Expression of class I, II, and IV chitinase genes was not enhanced, whereas expression of three class III chitinase genes, with almost no basal expression, was strongly induced. Two of these three (Mtchitinase III-2 and Mtchitinase III-3) were not induced at all in interactions with pathogens and rhizobia. Thus, the expression of two mycorrhiza-specific class III chitinase genes can be considered a hallmark for the establishment of arbuscular mycorrhiza in Medicago truncatula. FAU - Salzer, P AU - Salzer P AD - Botanisches Institut der Universitat Basel, Switzerland. Peter.Salzer@unibas.ch FAU - Bonanomi, A AU - Bonanomi A FAU - Beyer, K AU - Beyer K FAU - Vogeli-Lange, R AU - Vogeli-Lange R FAU - Aeschbacher, R A AU - Aeschbacher RA FAU - Lange, J AU - Lange J FAU - Wiemken, A AU - Wiemken A FAU - Kim, D AU - Kim D FAU - Cook, D R AU - Cook DR FAU - Boller, T AU - Boller T LA - eng SI - GENBANK/AF167322 SI - GENBANK/AF167323 SI - GENBANK/AF167324 SI - GENBANK/AF167325 SI - GENBANK/AF167326 SI - GENBANK/AF167327 SI - GENBANK/AF167328 SI - GENBANK/AF167329 SI - GENBANK/AJ238651 SI - GENBANK/AJ245511 SI - GENBANK/AJ245512 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mol Plant Microbe Interact JT - Molecular plant-microbe interactions : MPMI JID - 9107902 RN - 0 (DNA Primers) RN - 0 (Isoenzymes) RN - 0 (Ubiquitins) RN - EC 3.2.1.14 (Chitinases) SB - IM MH - Amino Acid Sequence MH - Base Sequence MH - Chitinases/biosynthesis/chemistry/*genetics MH - DNA Primers MH - Enzyme Induction MH - Fusarium/*pathogenicity MH - Gene Expression Regulation, Enzymologic MH - *Gene Expression Regulation, Plant MH - Isoenzymes/biosynthesis/chemistry/genetics MH - Medicago sativa/enzymology/*genetics/*microbiology MH - Molecular Sequence Data MH - Phytophthora/*pathogenicity MH - Plant Diseases MH - Plant Roots/enzymology/microbiology MH - Polymerase Chain Reaction MH - Sequence Alignment MH - Sequence Homology, Nucleic Acid MH - Ubiquitins/genetics EDAT- 2000/06/30 11:00 MHDA- 2001/02/28 10:01 CRDT- 2000/06/30 11:00 PHST- 2000/06/30 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2000/06/30 11:00 [entrez] AID - 10.1094/MPMI.2000.13.7.763 [doi] PST - ppublish SO - Mol Plant Microbe Interact. 2000 Jul;13(7):763-77. doi: 10.1094/MPMI.2000.13.7.763. ##### PUB RECORD ##### ## 10.1371/journal.pone.0247170 33606812 Hasan, Singh et al. 2021 "Hasan MS, Singh V, Islam S, Islam MS, Ahsan R, Kaundal A, Islam T, Ghosh A. Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L. PLoS One. 2021 Feb 19;16(2):e0247170. doi: 10.1371/journal.pone.0247170. PMID: 33606812; PMCID: PMC7894904." ## PMID- 33606812 OWN - NLM STAT- MEDLINE DCOM- 20210823 LR - 20231110 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 16 IP - 2 DP - 2021 TI - Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L. PG - e0247170 LID - 10.1371/journal.pone.0247170 [doi] LID - e0247170 AB - Glutathione transferases (GSTs) constitute an ancient, ubiquitous, multi-functional antioxidant enzyme superfamily that has great importance on cellular detoxification against abiotic and biotic stresses as well as plant development and growth. The present study aimed to a comprehensive genome-wide identification and functional characterization of GST family in one of the economically important legume plants-Medicago truncatula. Here, we have identified a total of ninety-two putative MtGST genes that code for 120 proteins. All these members were classified into twelve classes based on their phylogenetic relationship and the presence of structural conserved domain/motif. Among them, 7 MtGST gene pairs were identified to have segmental duplication. Expression profiling of MtGST transcripts revealed their high level of organ/tissue-specific expression in most of the developmental stages and anatomical tissues. The transcripts of MtGSTU5, MtGSTU8, MtGSTU17, MtGSTU46, and MtGSTU47 showed significant up-regulation in response to various abiotic and biotic stresses. Moreover, transcripts of MtGSTU8, MtGSTU14, MtGSTU28, MtGSTU30, MtGSTU34, MtGSTU46 and MtGSTF8 were found to be highly upregulated in response to drought treatment for 24h and 48h. Among the highly stress-responsive MtGST members, MtGSTU17 showed strong affinity towards its conventional substrates reduced glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) with the lowest binding energy of-5.7 kcal/mol and -6.5 kcal/mol, respectively. Furthermore, the substrate-binding site residues of MtGSTU17 were found to be highly conserved. These findings will facilitate the further functional and evolutionary characterization of GST genes in Medicago. FAU - Hasan, Md Soyib AU - Hasan MS AD - Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. FAU - Singh, Vishal AU - Singh V AD - Department of Plants, Soils, and Climate, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, United States of America. FAU - Islam, Shiful AU - Islam S AUID- ORCID: 0000-0002-1826-6777 AD - Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. FAU - Islam, Md Sifatul AU - Islam MS AUID- ORCID: 0000-0002-3971-6343 AD - Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. FAU - Ahsan, Raju AU - Ahsan R AD - Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. FAU - Kaundal, Amita AU - Kaundal A AUID- ORCID: 0000-0002-9154-1173 AD - Department of Plants, Soils, and Climate, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, United States of America. FAU - Islam, Tahmina AU - Islam T AD - Department of Botany, University of Dhaka, Dhaka, Bangladesh. FAU - Ghosh, Ajit AU - Ghosh A AUID- ORCID: 0000-0001-9045-3916 AD - Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. LA - eng PT - Journal Article DEP - 20210219 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Plant Proteins) RN - 0 (Protein Isoforms) RN - EC 2.5.1.18 (Glutathione Transferase) RN - GAN16C9B8O (Glutathione) SB - IM MH - Chromosomes, Plant/metabolism MH - Evolution, Molecular MH - Gene Duplication MH - Glutathione/chemistry/metabolism MH - Glutathione Transferase/classification/genetics/*metabolism MH - Glycosylation MH - Medicago truncatula/*enzymology/genetics/growth & development MH - Microsatellite Repeats/genetics MH - Molecular Docking Simulation MH - Phylogeny MH - Plant Proteins/classification/genetics/*metabolism MH - Protein Isoforms/classification/genetics/metabolism MH - Protein Structure, Tertiary MH - *Stress, Physiological MH - Transcriptome PMC - PMC7894904 COIS- The authors have declared that no competing interests exist. EDAT- 2021/02/20 06:00 MHDA- 2021/08/24 06:00 PMCR- 2021/02/19 CRDT- 2021/02/19 17:13 PHST- 2020/05/11 00:00 [received] PHST- 2021/02/02 00:00 [accepted] PHST- 2021/02/19 17:13 [entrez] PHST- 2021/02/20 06:00 [pubmed] PHST- 2021/08/24 06:00 [medline] PHST- 2021/02/19 00:00 [pmc-release] AID - PONE-D-20-13897 [pii] AID - 10.1371/journal.pone.0247170 [doi] PST - epublish SO - PLoS One. 2021 Feb 19;16(2):e0247170. doi: 10.1371/journal.pone.0247170. eCollection 2021. ##### PUB RECORD ##### ## 10.1371/journal.pone.0103770 25105497 Serwatowska, Roque et al., 2014 "Serwatowska J, Roque E, Gómez-Mena C, Constantin GD, Wen J, Mysore KS, Lund OS, Johansen E, Beltrán JP, Cañas LA. Two euAGAMOUS genes control C-function in Medicago truncatula. PLoS One. 2014 Aug 8;9(8):e103770. doi: 10.1371/journal.pone.0103770. PMID: 25105497; PMCID: PMC4126672." ## PMID- 25105497 OWN - NLM STAT- MEDLINE DCOM- 20150413 LR - 20211021 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 9 IP - 8 DP - 2014 TI - Two euAGAMOUS genes control C-function in Medicago truncatula. PG - e103770 LID - 10.1371/journal.pone.0103770 [doi] LID - e103770 AB - C-function MADS-box transcription factors belong to the AGAMOUS (AG) lineage and specify both stamen and carpel identity and floral meristem determinacy. In core eudicots, the AG lineage is further divided into two branches, the euAG and PLE lineages. Functional analyses across flowering plants strongly support the idea that duplicated AG lineage genes have different degrees of subfunctionalization of the C-function. The legume Medicago truncatula contains three C-lineage genes in its genome: two euAG genes (MtAGa and MtAGb) and one PLENA-like gene (MtSHP). This species is therefore a good experimental system to study the effects of gene duplication within the AG subfamily. We have studied the respective functions of each euAG genes in M. truncatula employing expression analyses and reverse genetic approaches. Our results show that the M. truncatula euAG- and PLENA-like genes are an example of subfunctionalization as a result of a change in expression pattern. MtAGa and MtAGb are the only genes showing a full C-function activity, concomitant with their ancestral expression profile, early in the floral meristem, and in the third and fourth floral whorls during floral development. In contrast, MtSHP expression appears late during floral development suggesting it does not contribute significantly to the C-function. Furthermore, the redundant MtAGa and MtAGb paralogs have been retained which provides the overall dosage required to specify the C-function in M. truncatula. FAU - Serwatowska, Joanna AU - Serwatowska J AD - Instituto de Biologia Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politecnica de la Innovacion, Valencia, Spain. FAU - Roque, Edelin AU - Roque E AD - Instituto de Biologia Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politecnica de la Innovacion, Valencia, Spain. FAU - Gomez-Mena, Concepcion AU - Gomez-Mena C AD - Instituto de Biologia Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politecnica de la Innovacion, Valencia, Spain. FAU - Constantin, Gabriela D AU - Constantin GD AD - Department of Plant Biology, Danish Institute of Agricultural Sciences, Frederiksberg C, Denmark. FAU - Wen, Jiangqi AU - Wen J AD - Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, United States of America. FAU - Mysore, Kirankumar S AU - Mysore KS AD - Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, United States of America. FAU - Lund, Ole S AU - Lund OS AD - Department of Plant Biology, Danish Institute of Agricultural Sciences, Frederiksberg C, Denmark. FAU - Johansen, Elisabeth AU - Johansen E AD - Department of Plant Biology, Danish Institute of Agricultural Sciences, Frederiksberg C, Denmark. FAU - Beltran, Jose Pio AU - Beltran JP AD - Instituto de Biologia Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politecnica de la Innovacion, Valencia, Spain. FAU - Canas, Luis A AU - Canas LA AD - Instituto de Biologia Molecular y Celular de Plantas (CSIC-UPV). Ciudad Politecnica de la Innovacion, Valencia, Spain. LA - eng SI - GENBANK/KF159804 SI - GENBANK/KF159805 SI - GENBANK/KJ470633 SI - GENBANK/KJ470634 SI - GENBANK/KJ470635 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140808 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (MADS Domain Proteins) SB - IM MH - Base Sequence MH - Blotting, Southern MH - Cluster Analysis MH - Flowers/*genetics/growth & development MH - Gene Silencing MH - Genes, Plant/*genetics MH - In Situ Hybridization MH - MADS Domain Proteins/*genetics MH - Medicago truncatula/*genetics/physiology MH - Molecular Sequence Data MH - Phylogeny MH - Polymerase Chain Reaction MH - RNA Interference MH - Real-Time Polymerase Chain Reaction MH - Reverse Transcriptase Polymerase Chain Reaction MH - Sequence Analysis, DNA PMC - PMC4126672 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2014/08/12 06:00 MHDA- 2015/04/14 06:00 PMCR- 2014/08/08 CRDT- 2014/08/09 06:00 PHST- 2014/06/06 00:00 [received] PHST- 2014/07/02 00:00 [accepted] PHST- 2014/08/09 06:00 [entrez] PHST- 2014/08/12 06:00 [pubmed] PHST- 2015/04/14 06:00 [medline] PHST- 2014/08/08 00:00 [pmc-release] AID - PONE-D-14-25339 [pii] AID - 10.1371/journal.pone.0103770 [doi] PST - epublish SO - PLoS One. 2014 Aug 8;9(8):e103770. doi: 10.1371/journal.pone.0103770. eCollection 2014. ##### PUB RECORD ##### ## 10.1105/tpc.111.089128 22080596 Peng, Yu et al., 2011 "Peng J, Yu J, Wang H, Guo Y, Li G, Bai G, Chen R. Regulation of compound leaf development in Medicago truncatula by fused compound leaf1, a class M KNOX gene. Plant Cell. 2011 Nov;23(11):3929-43. doi: 10.1105/tpc.111.089128. Epub 2011 Nov 11. PMID: 22080596; PMCID: PMC3246329." ## PMID- 22080596 OWN - NLM STAT- MEDLINE DCOM- 20120821 LR - 20240109 IS - 1532-298X (Electronic) IS - 1040-4651 (Print) IS - 1040-4651 (Linking) VI - 23 IP - 11 DP - 2011 Nov TI - Regulation of compound leaf development in Medicago truncatula by fused compound leaf1, a class M KNOX gene. PG - 3929-43 LID - 10.1105/tpc.111.089128 [doi] AB - Medicago truncatula is a legume species belonging to the inverted repeat lacking clade (IRLC) with trifoliolate compound leaves. However, the regulatory mechanisms underlying development of trifoliolate leaves in legumes remain largely unknown. Here, we report isolation and characterization of fused compound leaf1 (fcl1) mutants of M. truncatula. Phenotypic analysis suggests that FCL1 plays a positive role in boundary separation and proximal-distal axis development of compound leaves. Map-based cloning indicates that FCL1 encodes a class M KNOX protein that harbors the MEINOX domain but lacks the homeodomain. Yeast two-hybrid assays show that FCL1 interacts with a subset of Arabidopsis thaliana BEL1-like proteins with slightly different substrate specificities from the Arabidopsis homolog KNATM-B. Double mutant analyses with M. truncatula single leaflet1 (sgl1) and palmate-like pentafoliata1 (palm1) leaf mutants show that fcl1 is epistatic to palm1 and sgl1 is epistatic to fcl1 in terms of leaf complexity and that SGL1 and FCL1 act additively and are required for petiole development. Previous studies have shown that the canonical KNOX proteins are not involved in compound leaf development in IRLC legumes. The identification of FCL1 supports the role of a truncated KNOX protein in compound leaf development in M. truncatula. FAU - Peng, Jianling AU - Peng J AD - Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, USA. FAU - Yu, Jianbin AU - Yu J FAU - Wang, Hongliang AU - Wang H FAU - Guo, Yingqing AU - Guo Y FAU - Li, Guangming AU - Li G FAU - Bai, Guihua AU - Bai G FAU - Chen, Rujin AU - Chen R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20111111 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 RN - 0 (Arabidopsis Proteins) RN - 0 (BEL1 protein, Arabidopsis) RN - 0 (Homeodomain Proteins) RN - 0 (Plant Proteins) RN - 0 (Transcription Factors) SB - IM MH - Arabidopsis Proteins/genetics MH - Cloning, Molecular MH - Epistasis, Genetic MH - Gene Expression Regulation, Plant MH - Genes, Plant MH - Homeodomain Proteins/genetics MH - Medicago truncatula/*genetics/*growth & development MH - Mutation MH - Plant Leaves/genetics/*growth & development MH - Plant Proteins/*genetics/*metabolism MH - Sequence Homology, Nucleic Acid MH - Transcription Factors/genetics MH - Two-Hybrid System Techniques PMC - PMC3246329 EDAT- 2011/11/15 06:00 MHDA- 2012/08/22 06:00 PMCR- 2012/11/01 CRDT- 2011/11/15 06:00 PHST- 2011/11/15 06:00 [entrez] PHST- 2011/11/15 06:00 [pubmed] PHST- 2012/08/22 06:00 [medline] PHST- 2012/11/01 00:00 [pmc-release] AID - tpc.111.089128 [pii] AID - 089128 [pii] AID - 10.1105/tpc.111.089128 [doi] PST - ppublish SO - Plant Cell. 2011 Nov;23(11):3929-43. doi: 10.1105/tpc.111.089128. Epub 2011 Nov 11. ##### PUB RECORD ##### ## 10.3389/fpls.2016.00034 26858743 Qiao, Pingault et al., 2016 "Qiao Z, Pingault L, Nourbakhsh-Rey M, Libault M. Comprehensive Comparative Genomic and Transcriptomic Analyses of the Legume Genes Controlling the Nodulation Process. Front Plant Sci. 2016 Jan 29;7:34. doi: 10.3389/fpls.2016.00034. PMID: 26858743; PMCID: PMC4732000." ## PMID- 26858743 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20160209 LR - 20200930 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 7 DP - 2016 TI - Comprehensive Comparative Genomic and Transcriptomic Analyses of the Legume Genes Controlling the Nodulation Process. PG - 34 LID - 10.3389/fpls.2016.00034 [doi] LID - 34 AB - Nitrogen is one of the most essential plant nutrients and one of the major factors limiting crop productivity. Having the goal to perform a more sustainable agriculture, there is a need to maximize biological nitrogen fixation, a feature of legumes. To enhance our understanding of the molecular mechanisms controlling the interaction between legumes and rhizobia, the symbiotic partner fixing and assimilating the atmospheric nitrogen for the plant, researchers took advantage of genetic and genomic resources developed across different legume models (e.g., Medicago truncatula, Lotus japonicus, Glycine max, and Phaseolus vulgaris) to identify key regulatory protein coding genes of the nodulation process. In this study, we are presenting the results of a comprehensive comparative genomic analysis to highlight orthologous and paralogous relationships between the legume genes controlling nodulation. Mining large transcriptomic datasets, we also identified several orthologous and paralogous genes characterized by the induction of their expression during nodulation across legume plant species. This comprehensive study prompts new insights into the evolution of the nodulation process in legume plant and will benefit the scientific community interested in the transfer of functional genomic information between species. FAU - Qiao, Zhenzhen AU - Qiao Z AD - Department of Microbiology and Plant Biology, University of Oklahoma Norman, OK, USA. FAU - Pingault, Lise AU - Pingault L AD - Department of Microbiology and Plant Biology, University of Oklahoma Norman, OK, USA. FAU - Nourbakhsh-Rey, Mehrnoush AU - Nourbakhsh-Rey M AD - Department of Microbiology and Plant Biology, University of Oklahoma Norman, OK, USA. FAU - Libault, Marc AU - Libault M AD - Department of Microbiology and Plant Biology, University of Oklahoma Norman, OK, USA. LA - eng PT - Journal Article DEP - 20160129 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC4732000 OTO - NOTNLM OT - comparative genomic OT - comparative transcriptomic OT - legume OT - neo-/sub-functionalization OT - nodulation OT - orthologs OT - paralogs OT - root hair cell EDAT- 2016/02/10 06:00 MHDA- 2016/02/10 06:01 PMCR- 2016/01/01 CRDT- 2016/02/10 06:00 PHST- 2015/10/28 00:00 [received] PHST- 2016/01/10 00:00 [accepted] PHST- 2016/02/10 06:00 [entrez] PHST- 2016/02/10 06:00 [pubmed] PHST- 2016/02/10 06:01 [medline] PHST- 2016/01/01 00:00 [pmc-release] AID - 10.3389/fpls.2016.00034 [doi] PST - epublish SO - Front Plant Sci. 2016 Jan 29;7:34. doi: 10.3389/fpls.2016.00034. eCollection 2016. ##### PUB RECORD ##### ## 10.1007/s11103-005-8102-y 16240175 Schnabel Journet et al., 2005 "Schnabel E, Journet EP, de Carvalho-Niebel F, Duc G, Frugoli J. The Medicago truncatula SUNN gene encodes a CLV1-like leucine-rich repeat receptor kinase that regulates nodule number and root length. Plant Mol Biol. 2005 Aug;58(6):809-822. doi: 10.1007/s11103-005-8102-y. PMID: 16240175." ## PMID- 16240175 OWN - NLM STAT- MEDLINE DCOM- 20060110 LR - 20240109 IS - 0167-4412 (Print) IS - 0167-4412 (Linking) VI - 58 IP - 6 DP - 2005 Aug TI - The Medicago truncatula SUNN gene encodes a CLV1-like leucine-rich repeat receptor kinase that regulates nodule number and root length. PG - 809-822 LID - 10.1007/s11103-005-8102-y [doi] AB - Four Medicago truncatula sunn mutants displayed shortened roots and hypernodulation under all conditions examined. The mutants, recovered in three independent genetic screens, all contained lesions in a leucine-rich repeat (LRR) receptor kinase. Although the molecular defects among alleles varied, root length and the extent of nodulation were not significantly different between the mutants. SUNN is expressed in shoots, flowers and roots. Although previously reported grafting experiments showed that the presence of the mutated SUNN gene in roots does not confer an obvious phenotype, expression levels of SUNN mRNA were reduced in sunn-1 roots. SUNN and the previously identified genes HAR1 (Lotus japonicus) and NARK (Glycine max) are orthologs based on gene sequence and synteny between flanking sequences. Comparison of related LRR receptor kinases determined that all nodulation autoregulation genes identified to date are the closest legume relatives of AtCLV1 by sequence, yet sunn, har and nark mutants do not display the fasciated clv phenotype. The M. truncatula region is syntenic with duplicated regions of Arabidopsis chromosomes 2 and 4, none of which harbor CLV1 or any other LRR receptor kinase genes. A novel truncated copy of the SUNN gene lacking a kinase domain, RLP1, is found immediately upstream of SUNN and like SUNN is expressed at a reduced level in sunn-1 roots. FAU - Schnabel, Elise AU - Schnabel E AD - Department of Genetics, Biochemistry and Life Science Studies, Clemson University, 100 Jordan Hall, Clemson, SC, 29634, USA. FAU - Journet, Etienne-Pascal AU - Journet EP AD - Laboratoire des Interactions Plantes-Microorganismes, Unite Mixte de Recherche, CNRS-INRA, 31326, Castanet-Tolosan cedex, France. FAU - de Carvalho-Niebel, Fernanda AU - de Carvalho-Niebel F AD - Laboratoire des Interactions Plantes-Microorganismes, Unite Mixte de Recherche, CNRS-INRA, 31326, Castanet-Tolosan cedex, France. FAU - Duc, Gerard AU - Duc G AD - lNRA Unite de Recherches en Genetique et Ecophysiologie des legumineuses a graines, BP 86510, F-21065, DIJON cedex, France. FAU - Frugoli, Julia AU - Frugoli J AD - Department of Genetics, Biochemistry and Life Science Studies, Clemson University, 100 Jordan Hall, Clemson, SC, 29634, USA. jfrugol@CLEMSON.EDU. LA - eng PT - Journal Article PL - Netherlands TA - Plant Mol Biol JT - Plant molecular biology JID - 9106343 RN - 0 (Nitrates) RN - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) SB - IM MH - Gene Expression Regulation, Enzymologic MH - Gene Expression Regulation, Plant MH - Genes, Plant/*genetics MH - Medicago truncatula/drug effects/*enzymology/*genetics MH - Mutation MH - Nitrates/pharmacology MH - Phenotype MH - Phylogeny MH - Plant Roots/drug effects/genetics/*growth & development MH - Plant Shoots/genetics/metabolism MH - Receptor Protein-Tyrosine Kinases/*chemistry/genetics/*metabolism MH - Synteny EDAT- 2005/10/22 09:00 MHDA- 2006/01/13 09:00 CRDT- 2005/10/22 09:00 PHST- 2005/03/16 00:00 [received] PHST- 2005/05/29 00:00 [accepted] PHST- 2005/10/22 09:00 [pubmed] PHST- 2006/01/13 09:00 [medline] PHST- 2005/10/22 09:00 [entrez] AID - 10.1007/s11103-005-8102-y [pii] AID - 10.1007/s11103-005-8102-y [doi] PST - ppublish SO - Plant Mol Biol. 2005 Aug;58(6):809-822. doi: 10.1007/s11103-005-8102-y. ##### PUB RECORD ##### ## 10.1111/tpj.13234 27296908 Crook, Schnabel et al., 2016 "Crook AD, Schnabel EL, Frugoli JA. The systemic nodule number regulation kinase SUNN in Medicago truncatula interacts with MtCLV2 and MtCRN. Plant J. 2016 Oct;88(1):108-119. doi: 10.1111/tpj.13234. Epub 2016 Aug 21. PMID: 27296908." ## PMID- 27296908 OWN - NLM STAT- MEDLINE DCOM- 20171020 LR - 20220408 IS - 1365-313X (Electronic) IS - 0960-7412 (Linking) VI - 88 IP - 1 DP - 2016 Oct TI - The systemic nodule number regulation kinase SUNN in Medicago truncatula interacts with MtCLV2 and MtCRN. PG - 108-119 LID - 10.1111/tpj.13234 [doi] AB - Autoregulation of nodulation (AON), a systemic signaling pathway in legumes, limits the number of nodules formed by the legume in its symbiosis with rhizobia. Recent research suggests a model for the systemic regulation in Medicago truncatula in which root signaling peptides are translocated to the shoot where they bind to a shoot receptor complex containing the leucine-rich repeat receptor-like kinase SUNN, triggering signal transduction which terminates nodule formation in roots. Here we show that a tagged SUNN protein capable of rescuing the sunn-4 phenotype is localized to the plasma membrane and is associated with the plasmodesmata. Using bimolecular fluorescence complementation analysis we show that, like its sequence ortholog Arabidopsis CLV1, SUNN interacts with homologous CLV1-interacting proteins MtCLAVATA2 and MtCORYNE. All three proteins were also able to form homomers and MtCRN and MtCLV2 also interact with each other. A crn Tnt1 insertion mutant of M. truncatula displayed a shoot controlled increased nodulation phenotype, similar to the clv2 mutants of pea and Lotus japonicus. Together these data suggest that legume AON signaling could occur through a multi-protein complex and that both MtCRN and MtCLV2 may play roles in AON together with SUNN. CI - (c) 2016 The Authors The Plant Journal (c) 2016 John Wiley & Sons Ltd. FAU - Crook, Ashley D AU - Crook AD AD - Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29630-0318, USA. FAU - Schnabel, Elise L AU - Schnabel EL AD - Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29630-0318, USA. FAU - Frugoli, Julia A AU - Frugoli JA AD - Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29630-0318, USA. jfrugol@clemson.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20160821 PL - England TA - Plant J JT - The Plant journal : for cell and molecular biology JID - 9207397 RN - 0 (Plant Proteins) SB - IM MH - Gene Expression Regulation, Plant/genetics/physiology MH - Medicago truncatula/genetics/*metabolism MH - Plant Proteins/genetics/*metabolism MH - Protein Binding MH - Root Nodules, Plant/genetics/*metabolism OTO - NOTNLM OT - Medicago truncatula OT - CLAVATA2 OT - CORYNE OT - autoregulation of nodulation OT - bimolecular fluorescence complementation OT - protein-protein interactions OT - receptor OT - signal transduction OT - super numerary nodules EDAT- 2016/10/25 06:00 MHDA- 2017/10/21 06:00 CRDT- 2016/06/15 06:00 PHST- 2016/04/22 00:00 [received] PHST- 2016/06/01 00:00 [revised] PHST- 2016/06/03 00:00 [accepted] PHST- 2016/10/25 06:00 [pubmed] PHST- 2017/10/21 06:00 [medline] PHST- 2016/06/15 06:00 [entrez] AID - 10.1111/tpj.13234 [doi] PST - ppublish SO - Plant J. 2016 Oct;88(1):108-119. doi: 10.1111/tpj.13234. Epub 2016 Aug 21. ##### PUB RECORD ##### ## 10.1111/j.1365-313X.2006.02910.x 17132148 Gargantini, Gonzalez-Rizzo et al., 2006 "Gargantini PR, Gonzalez-Rizzo S, Chinchilla D, Raices M, Giammaria V, Ulloa RM, Frugier F, Crespi MD. A CDPK isoform participates in the regulation of nodule number in Medicago truncatula. Plant J. 2006 Dec;48(6):843-56. doi: 10.1111/j.1365-313X.2006.02910.x. Epub 2006 Nov 21. PMID: 17132148." ## PMID- 17132148 OWN - NLM STAT- MEDLINE DCOM- 20070417 LR - 20220408 IS - 0960-7412 (Print) IS - 0960-7412 (Linking) VI - 48 IP - 6 DP - 2006 Dec TI - A CDPK isoform participates in the regulation of nodule number in Medicago truncatula. PG - 843-56 AB - Medicago spp. are able to develop root nodules via symbiotic interaction with Sinorhizobium meliloti. Calcium-dependent protein kinases (CDPKs) are involved in various signalling pathways in plants, and we found that expression of MtCPK3, a CDPK isoform present in roots of the model legume Medicago truncatula, is regulated during the nodulation process. Early inductions were detected 15 min and 3-4 days post-inoculation (dpi). The very early induction of CPK3 messengers was also present in inoculated M. truncatula dmi mutants and in wild-type roots subjected to salt stress, indicating that this rapid response is probably stress-related. In contrast, the later response was concomitant with cortical cell division and the formation of nodule primordia, and was not observed in wild-type roots inoculated with nod (-) strains. This late induction correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3 antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3 participates in the regulation of the symbiotic interaction. FAU - Gargantini, Pablo R AU - Gargantini PR AD - Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, Vuelta de Obligado 2490, 2 piso, 1428 Buenos Aires, Argentina. FAU - Gonzalez-Rizzo, Silvina AU - Gonzalez-Rizzo S FAU - Chinchilla, Delphine AU - Chinchilla D FAU - Raices, Marcela AU - Raices M FAU - Giammaria, Veronica AU - Giammaria V FAU - Ulloa, Rita M AU - Ulloa RM FAU - Frugier, Florian AU - Frugier F FAU - Crespi, Martin D AU - Crespi MD LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20061121 PL - England TA - Plant J JT - The Plant journal : for cell and molecular biology JID - 9207397 RN - 0 (Cytokinins) RN - 0 (Isoenzymes) RN - 0 (Plant Proteins) RN - 0 (RNA, Messenger) RN - 0 (RNA, Plant) RN - 147336-22-9 (Green Fluorescent Proteins) RN - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases) SB - IM MH - Calcium-Calmodulin-Dependent Protein Kinases/genetics/*metabolism MH - Cytokinins/pharmacology MH - Gene Expression Regulation, Enzymologic MH - Gene Expression Regulation, Plant MH - Genes, Plant MH - Green Fluorescent Proteins/genetics MH - Isoenzymes/genetics/metabolism MH - Medicago sativa/enzymology MH - Medicago truncatula/*enzymology/genetics/microbiology MH - Onions/cytology MH - Plant Proteins/genetics/*metabolism MH - Plant Roots/*enzymology/microbiology MH - RNA Interference MH - RNA, Messenger MH - RNA, Plant MH - Rhizobium/enzymology MH - Sinorhizobium meliloti/physiology MH - Symbiosis/*physiology MH - Up-Regulation EDAT- 2006/11/30 09:00 MHDA- 2007/04/18 09:00 CRDT- 2006/11/30 09:00 PHST- 2006/11/30 09:00 [pubmed] PHST- 2007/04/18 09:00 [medline] PHST- 2006/11/30 09:00 [entrez] AID - TPJ2910 [pii] AID - 10.1111/j.1365-313X.2006.02910.x [doi] PST - ppublish SO - Plant J. 2006 Dec;48(6):843-56. doi: 10.1111/j.1365-313X.2006.02910.x. Epub 2006 Nov 21. ##### PUB RECORD ##### ## 10.1093/jxb/erw474 28073951 Herrbach, Chirinos et al., 2017 "Herrbach V, Chirinos X, Rengel D, Agbevenou K, Vincent R, Pateyron S, Huguet S, Balzergue S, Pasha A, Provart N, Gough C, Bensmihen S. Nod factors potentiate auxin signaling for transcriptional regulation and lateral root formation in Medicago truncatula. J Exp Bot. 2017 Jan 1;68(3):569-583. doi: 10.1093/jxb/erw474. PMID: 28073951; PMCID: PMC6055581." ## PMID- 28073951 OWN - NLM STAT- MEDLINE DCOM- 20171117 LR - 20210109 IS - 1460-2431 (Electronic) IS - 0022-0957 (Print) IS - 0022-0957 (Linking) VI - 68 IP - 3 DP - 2017 Jan 1 TI - Nod factors potentiate auxin signaling for transcriptional regulation and lateral root formation in Medicago truncatula. PG - 569-583 LID - 10.1093/jxb/erw474 [doi] AB - Nodulation (Nod) factors (NFs) are symbiotic molecules produced by rhizobia that are essential for establishment of the rhizobium-legume endosymbiosis. Purified NFs can stimulate lateral root formation (LRF) in Medicago truncatula, but little is known about the molecular mechanisms involved. Using a combination of reporter constructs, pharmacological and genetic approaches, we show that NFs act on early steps of LRF in M. truncatula, independently of the ethylene signaling pathway and of the cytokinin receptor MtCRE1, but in interaction with auxin. We conducted a whole-genome transcriptomic study upon NF and/or auxin treatments, using a lateral root inducible system adapted for M. truncatula. This revealed a large overlap between NF and auxin signaling and, more interestingly, synergistic interactions between these molecules. Three groups showing interaction effects were defined: group 1 contained more than 1500 genes responding specifically to the combinatorial treatment of NFs and auxin; group 2 comprised auxin-regulated genes whose expression was enhanced or antagonized by NFs; and in group 3 the expression of NF regulated genes was antagonized by auxin. Groups 1 and 2 were enriched in signaling and metabolic functions, which highlights important crosstalk between NF and auxin signaling for both developmental and symbiotic processes. CI - (c) The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. FAU - Herrbach, Violaine AU - Herrbach V AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Chirinos, Ximena AU - Chirinos X AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Rengel, David AU - Rengel D AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Agbevenou, Kokoevi AU - Agbevenou K AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Vincent, Remy AU - Vincent R AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Pateyron, Stephanie AU - Pateyron S AD - POPS (transcriptOmic Platform of IPS2) Platform, Institute of Plant Sciences Paris Saclay (IPS2), CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Saclay, Orsay, France. AD - Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cite, Orsay, France. FAU - Huguet, Stephanie AU - Huguet S AD - POPS (transcriptOmic Platform of IPS2) Platform, Institute of Plant Sciences Paris Saclay (IPS2), CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Saclay, Orsay, France. AD - Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cite, Orsay, France. FAU - Balzergue, Sandrine AU - Balzergue S AD - POPS (transcriptOmic Platform of IPS2) Platform, Institute of Plant Sciences Paris Saclay (IPS2), CNRS, INRA, Universite Paris-Sud, Universite Evry, Universite Paris-Saclay, Orsay, France. AD - Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cite, Orsay, France. FAU - Pasha, Asher AU - Pasha A AD - Department of Cell & Systems Biology/ Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Canada. FAU - Provart, Nicholas AU - Provart N AD - Department of Cell & Systems Biology/ Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Canada. FAU - Gough, Clare AU - Gough C AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Bensmihen, Sandra AU - Bensmihen S AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. LA - eng PT - Journal Article PL - England TA - J Exp Bot JT - Journal of experimental botany JID - 9882906 RN - 0 (Indoleacetic Acids) RN - 0 (Lipopolysaccharides) RN - 0 (Plant Growth Regulators) SB - IM MH - *Gene Expression Regulation, Plant MH - Indoleacetic Acids/*metabolism MH - Lipopolysaccharides/*physiology MH - Medicago truncatula/genetics/growth & development/microbiology/*physiology MH - Plant Growth Regulators/*metabolism MH - Plant Roots/genetics/growth & development/microbiology MH - Sinorhizobium meliloti/*physiology PMC - PMC6055581 OTO - NOTNLM OT - Auxin OT - NimbleGen arrays OT - Nod factors OT - ethylene OT - lateral root OT - lateral root inducible system (LRIS) OT - lipo-chitooligosaccharides (LCOs) OT - symbiosis OT - transcriptome EDAT- 2017/01/12 06:00 MHDA- 2017/11/29 06:00 PMCR- 2017/01/10 CRDT- 2017/01/12 06:00 PHST- 2017/01/12 06:00 [pubmed] PHST- 2017/11/29 06:00 [medline] PHST- 2017/01/12 06:00 [entrez] PHST- 2017/01/10 00:00 [pmc-release] AID - erw474 [pii] AID - 10.1093/jxb/erw474 [doi] PST - ppublish SO - J Exp Bot. 2017 Jan 1;68(3):569-583. doi: 10.1093/jxb/erw474. ##### PUB RECORD ##### ## 10.1104/pp.15.00164 25792252 Weller, Foo et al., 2015 "Weller JL, Foo EM, Hecht V, Ridge S, Vander Schoor JK, Reid JB. Ethylene Signaling Influences Light-Regulated Development in Pea. Plant Physiol. 2015 Sep;169(1):115-24. doi: 10.1104/pp.15.00164. Epub 2015 Mar 19. PMID: 25792252; PMCID: PMC4577373." ## PMID- 25792252 OWN - NLM STAT- MEDLINE DCOM- 20160707 LR - 20240109 IS - 1532-2548 (Electronic) IS - 0032-0889 (Print) IS - 0032-0889 (Linking) VI - 169 IP - 1 DP - 2015 Sep TI - Ethylene Signaling Influences Light-Regulated Development in Pea. PG - 115-24 LID - 10.1104/pp.15.00164 [doi] AB - Plant responses to light involve a complex network of interactions among multiple plant hormones. In a screen for mutants showing altered photomorphogenesis under red light, we identified a mutant with dramatically enhanced leaf expansion and delayed petal senescence. We show that this mutant exhibits reduced sensitivity to ethylene and carries a nonsense mutation in the single pea (Pisum sativum) ortholog of the ethylene signaling gene ETHYLENE INSENSITIVE2 (EIN2). Consistent with this observation, the ein2 mutation rescues the previously described effects of ethylene overproduction in mature phytochrome-deficient plants. In seedlings, ein2 confers a marked increase in leaf expansion under monochromatic red, far-red, or blue light, and interaction with phytochromeA, phytochromeB, and long1 mutants confirms that ein2 enhances both phytochrome- and cryptochrome-dependent responses in a LONG1-dependent manner. In contrast, minimal effects of ein2 on seedling development in darkness or high-irradiance white light show that ethylene is not limiting for development under these conditions. These results indicate that ethylene signaling constrains leaf expansion during deetiolation in pea and provide further evidence that down-regulation of ethylene production may be an important component mechanism in the broader control of photomorphogenic development by phytochrome and cryptochrome. CI - (c) 2015 American Society of Plant Biologists. All Rights Reserved. FAU - Weller, James L AU - Weller JL AUID- ORCID: 0000-0003-2423-8286 AD - School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia jim.weller@utas.edu.au. FAU - Foo, Eloise M AU - Foo EM AUID- ORCID: 0000-0002-9751-8433 AD - School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia. FAU - Hecht, Valerie AU - Hecht V AUID- ORCID: 0000-0002-3539-3356 AD - School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia. FAU - Ridge, Stephen AU - Ridge S AD - School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia. FAU - Vander Schoor, Jacqueline K AU - Vander Schoor JK AD - School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia. FAU - Reid, James B AU - Reid JB AD - School of Biological Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia. LA - eng SI - GENBANK/KP202149 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150319 PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 RN - 0 (Cryptochromes) RN - 0 (Ethylenes) RN - 0 (Plant Growth Regulators) RN - 0 (Plant Proteins) RN - 11121-56-5 (Phytochrome) RN - 91GW059KN7 (ethylene) SB - IM MH - Cryptochromes/metabolism MH - Darkness MH - Down-Regulation MH - Ethylenes/*metabolism MH - Gene Expression Regulation, Developmental MH - Gene Expression Regulation, Plant MH - Light MH - Molecular Sequence Data MH - Mutation MH - Pisum sativum/genetics/growth & development/*physiology/radiation effects MH - Phytochrome/*metabolism MH - Plant Growth Regulators/*metabolism MH - Plant Leaves/genetics/growth & development/physiology/radiation effects MH - Plant Proteins/genetics/*metabolism MH - Seedlings/genetics/growth & development/physiology/radiation effects MH - Signal Transduction PMC - PMC4577373 EDAT- 2015/03/21 06:00 MHDA- 2016/07/09 06:00 PMCR- 2015/03/19 CRDT- 2015/03/21 06:00 PHST- 2015/02/02 00:00 [received] PHST- 2015/03/17 00:00 [accepted] PHST- 2015/03/21 06:00 [entrez] PHST- 2015/03/21 06:00 [pubmed] PHST- 2016/07/09 06:00 [medline] PHST- 2015/03/19 00:00 [pmc-release] AID - pp.15.00164 [pii] AID - PP201500164 [pii] AID - 10.1104/pp.15.00164 [doi] PST - ppublish SO - Plant Physiol. 2015 Sep;169(1):115-24. doi: 10.1104/pp.15.00164. Epub 2015 Mar 19. ##### PUB RECORD ##### ## 10.1111/j.1469-8137.2012.04147.x 22530598 Cheng, Peng et al., 2012 "Cheng X, Peng J, Ma J, Tang Y, Chen R, Mysore KS, Wen J. NO APICAL MERISTEM (MtNAM) regulates floral organ identity and lateral organ separation in Medicago truncatula. New Phytol. 2012 Jul;195(1):71-84. doi: 10.1111/j.1469-8137.2012.04147.x. Epub 2012 Apr 24. PMID: 22530598." ## PMID- 22530598 OWN - NLM STAT- MEDLINE DCOM- 20121009 LR - 20220331 IS - 1469-8137 (Electronic) IS - 0028-646X (Linking) VI - 195 IP - 1 DP - 2012 Jul TI - NO APICAL MERISTEM (MtNAM) regulates floral organ identity and lateral organ separation in Medicago truncatula. PG - 71-84 LID - 10.1111/j.1469-8137.2012.04147.x [doi] AB - * The CUP-SHAPED COTYLEDON (CUC)/NO APICAL MERISTEM (NAM) family of genes control boundary formation and lateral organ separation, which is critical for proper leaf and flower patterning. However, most downstream targets of CUC/NAM genes remain unclear. * In a forward screen of the tobacco retrotransposon1 (Tnt1) insertion population in Medicago truncatula, we isolated a weak allele of the no-apical-meristem mutant mtnam-2. Meanwhile, we regenerated a mature plant from the null allele mtnam-1. These materials allowed us to extensively characterize the function of MtNAM and its downstream genes. * MtNAM is highly expressed in vegetative shoot buds and inflorescence apices, specifically at boundaries between the shoot apical meristem and leaf/flower primordia. Mature plants of the regenerated null allele and the weak allele display remarkable floral phenotypes: floral whorls and organ numbers are reduced and the floral organ identity is compromised. Microarray and quantitative RT-PCR analyses revealed that all classes of floral homeotic genes are down-regulated in mtnam mutants. Mutations in MtNAM also lead to fused cotyledons and leaflets of the compound leaf as well as a defective shoot apical meristem. * Our results revealed that MtNAM shares the role of CUC/NAM family genes in lateral organ separation and compound leaf development, and is also required for floral organ identity and development. CI - (c) 2012 The Authors. New Phytologist (c) 2012 New Phytologist Trust. FAU - Cheng, Xiaofei AU - Cheng X AD - Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA. FAU - Peng, Jianling AU - Peng J FAU - Ma, Junying AU - Ma J FAU - Tang, Yuhong AU - Tang Y FAU - Chen, Rujin AU - Chen R FAU - Mysore, Kirankumar S AU - Mysore KS FAU - Wen, Jiangqi AU - Wen J LA - eng SI - GENBANK/JF929904 PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20120424 PL - England TA - New Phytol JT - The New phytologist JID - 9882884 RN - 0 (Plant Proteins) RN - 0 (Retroelements) SB - IM MH - Cotyledon/anatomy & histology/genetics MH - Flowers/genetics/*physiology MH - *Gene Expression Regulation, Plant MH - Genetic Association Studies MH - Inflorescence/anatomy & histology/genetics MH - Medicago truncatula/genetics/*physiology MH - Meristem/*genetics/physiology MH - Molecular Sequence Data MH - Mutation MH - Plant Leaves/genetics/growth & development MH - Plant Proteins/*genetics/*metabolism MH - Retroelements EDAT- 2012/04/26 06:00 MHDA- 2012/10/10 06:00 CRDT- 2012/04/26 06:00 PHST- 2012/04/26 06:00 [entrez] PHST- 2012/04/26 06:00 [pubmed] PHST- 2012/10/10 06:00 [medline] AID - 10.1111/j.1469-8137.2012.04147.x [doi] PST - ppublish SO - New Phytol. 2012 Jul;195(1):71-84. doi: 10.1111/j.1469-8137.2012.04147.x. Epub 2012 Apr 24. ##### PUB RECORD ##### ## 10.1104/pp.109.143024 19789288 Kuppusamy, Ivashuta et al., 2009 "Kuppusamy KT, Ivashuta S, Bucciarelli B, Vance CP, Gantt JS, Vandenbosch KA. Knockdown of CELL DIVISION CYCLE16 reveals an inverse relationship between lateral root and nodule numbers and a link to auxin in Medicago truncatula. Plant Physiol. 2009 Nov;151(3):1155-66. doi: 10.1104/pp.109.143024. Epub 2009 Sep 29. PMID: 19789288; PMCID: PMC2773094." ## PMID- 19789288 OWN - NLM STAT- MEDLINE DCOM- 20100127 LR - 20240314 IS - 1532-2548 (Electronic) IS - 0032-0889 (Print) IS - 0032-0889 (Linking) VI - 151 IP - 3 DP - 2009 Nov TI - Knockdown of CELL DIVISION CYCLE16 reveals an inverse relationship between lateral root and nodule numbers and a link to auxin in Medicago truncatula. PG - 1155-66 LID - 10.1104/pp.109.143024 [doi] AB - The postembryonic development of lateral roots and nodules is a highly regulated process. Recent studies suggest the existence of cross talk and interdependency in the growth of these two organs. Although plant hormones, including auxin and cytokinin, appear to be key players in coordinating this cross talk, very few genes that cross-regulate root and nodule development have been uncovered so far. This study reports that a homolog of CELL DIVISION CYCLE16 (CDC16), a core component of the Anaphase Promoting Complex, is one of the key mediators in controlling the overall number of lateral roots and nodules. A partial suppression of this gene in Medicago truncatula leads to a decrease in number of lateral roots and a 4-fold increase in number of nodules. The roots showing lowered expression of MtCDC16 also show reduced sensitivity to phytohormone auxin, thus providing a potential function of CDC16 in auxin signaling. FAU - Kuppusamy, Kavitha T AU - Kuppusamy KT AD - Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108, USA. FAU - Ivashuta, Sergey AU - Ivashuta S FAU - Bucciarelli, Bruna AU - Bucciarelli B FAU - Vance, Carroll P AU - Vance CP FAU - Gantt, J Stephen AU - Gantt JS FAU - Vandenbosch, Kathryn A AU - Vandenbosch KA LA - eng SI - GENBANK/GU075685 SI - GENBANK/GU075686 PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20090929 PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 RN - 0 (Cell Cycle Proteins) RN - 0 (DNA, Plant) RN - 0 (Indoleacetic Acids) RN - 0 (Plant Growth Regulators) RN - 0 (Plant Proteins) SB - IM MH - Amino Acid Sequence MH - Cell Cycle Proteins/genetics/*metabolism MH - DNA, Plant/genetics MH - Gene Expression Regulation, Plant MH - Gene Knockdown Techniques MH - Indoleacetic Acids/*metabolism MH - Medicago truncatula/cytology/*genetics/growth & development MH - Molecular Sequence Data MH - Oligonucleotide Array Sequence Analysis MH - Plant Growth Regulators/metabolism MH - Plant Proteins/genetics/*metabolism MH - Plant Root Nodulation/*genetics MH - Plants, Genetically Modified/cytology/genetics/growth & development MH - RNA Interference MH - Root Nodules, Plant/*growth & development MH - Sequence Analysis, DNA PMC - PMC2773094 EDAT- 2009/10/01 06:00 MHDA- 2010/01/28 06:00 PMCR- 2009/09/29 CRDT- 2009/10/01 06:00 PHST- 2009/10/01 06:00 [entrez] PHST- 2009/10/01 06:00 [pubmed] PHST- 2010/01/28 06:00 [medline] PHST- 2009/09/29 00:00 [pmc-release] AID - pp.109.143024 [pii] AID - 143024 [pii] AID - 10.1104/pp.109.143024 [doi] PST - ppublish SO - Plant Physiol. 2009 Nov;151(3):1155-66. doi: 10.1104/pp.109.143024. Epub 2009 Sep 29. ##### PUB RECORD ##### ## 10.1111/nph.12681 24443934 Mortier, Wasson et al., 2014 "Mortier V, Wasson A, Jaworek P, De Keyser A, Decroos M, Holsters M, Tarkowski P, Mathesius U, Goormachtig S. Role of LONELY GUY genes in indeterminate nodulation on Medicago truncatula. New Phytol. 2014 Apr;202(2):582-593. doi: 10.1111/nph.12681. Epub 2014 Jan 21. PMID: 24443934." ## PMID- 24443934 OWN - NLM STAT- MEDLINE DCOM- 20141103 LR - 20240109 IS - 1469-8137 (Electronic) IS - 0028-646X (Linking) VI - 202 IP - 2 DP - 2014 Apr TI - Role of LONELY GUY genes in indeterminate nodulation on Medicago truncatula. PG - 582-593 LID - 10.1111/nph.12681 [doi] AB - LONELY GUY (LOG) genes encode cytokinin riboside 5'-monophosphate phosphoribohydrolases and are directly involved in the activation of cytokinins. To assess whether LOG proteins affect the influence of cytokinin on nodulation, we studied two LOG genes of Medicago truncatula. Expression analysis showed that MtLOG1 and MtLOG2 were upregulated during nodulation in a CRE1-dependent manner. Expression was mainly localized in the dividing cells of the nodule primordium. In addition, RNA interference revealed that MtLOG1 is involved in nodule development and that the gene plays a negative role in lateral root development. Ectopic expression of MtLOG1 resulted in a change in cytokinin homeostasis, triggered cytokinin-inducible genes and produced roots with enlarged vascular tissues and shortened primary roots. In addition, those 35S:LOG1 roots also displayed fewer nodules than the wild-type. This inhibition in nodule formation was local, independent of the SUPER NUMERIC NODULES gene, but coincided with an upregulation of the MtCLE13 gene, encoding a CLAVATA3/EMBRYO SURROUNDING REGION peptide. In conclusion, we demonstrate that in M. truncatula LOG proteins might be implicated in nodule primordium development and lateral root formation. CI - (c) 2014 The Authors. New Phytologist (c) 2014 New Phytologist Trust. FAU - Mortier, Virginie AU - Mortier V AD - Department of Plant Systems Biology, VIB, 9052, Gent, Belgium. AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium. FAU - Wasson, Anton AU - Wasson A AD - Division of Plant Science, Research School of Biology, The Australian National University, Acton, ACT, 0200, Australia. FAU - Jaworek, Pavel AU - Jaworek P AD - Centre of the Region Hana for the Biotechnological and Agricultural Research, Faculty of Science, Palacky University, 783 71, Olomouc, Czech Republic. FAU - De Keyser, Annick AU - De Keyser A AD - Department of Plant Systems Biology, VIB, 9052, Gent, Belgium. AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium. FAU - Decroos, Martijn AU - Decroos M AD - Department of Plant Systems Biology, VIB, 9052, Gent, Belgium. AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium. FAU - Holsters, Marcelle AU - Holsters M AD - Department of Plant Systems Biology, VIB, 9052, Gent, Belgium. AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium. FAU - Tarkowski, Petr AU - Tarkowski P AD - Centre of the Region Hana for the Biotechnological and Agricultural Research, Faculty of Science, Palacky University, 783 71, Olomouc, Czech Republic. FAU - Mathesius, Ulrike AU - Mathesius U AD - Division of Plant Science, Research School of Biology, The Australian National University, Acton, ACT, 0200, Australia. FAU - Goormachtig, Sofie AU - Goormachtig S AD - Department of Plant Systems Biology, VIB, 9052, Gent, Belgium. AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140121 PL - England TA - New Phytol JT - The New phytologist JID - 9882884 RN - 0 (Arabidopsis Proteins) RN - 0 (Cytokinins) RN - 0 (Nuclear Proteins) RN - 0 (Plant Proteins) RN - 0 (Receptors, Cell Surface) RN - EC 2.7.- (Protein Kinases) RN - EC 2.7.3.- (WOL protein, Arabidopsis) RN - EC 3.5.4.- (Aminohydrolases) RN - EC 3.5.4.- (cytokinin riboside 5'-monophosphate phosphoribohydrolase, Arabidopsis) SB - IM MH - Aminohydrolases MH - Arabidopsis Proteins/genetics/metabolism MH - Cytokinins/*genetics/metabolism MH - *Gene Expression Regulation, Plant MH - *Genes, Plant MH - Medicago truncatula/*genetics/metabolism MH - Nuclear Proteins/genetics/metabolism MH - Plant Proteins/*genetics MH - Plant Root Nodulation/*genetics MH - Plant Roots/growth & development MH - Protein Kinases/genetics/metabolism MH - Receptors, Cell Surface/genetics/metabolism MH - Root Nodules, Plant/*growth & development MH - Up-Regulation OTO - NOTNLM OT - LONELY GUY OT - cytokinin OT - nodulation OT - nodule primordium OT - root growth EDAT- 2014/01/22 06:00 MHDA- 2014/11/05 06:00 CRDT- 2014/01/22 06:00 PHST- 2013/09/11 00:00 [received] PHST- 2013/12/11 00:00 [accepted] PHST- 2014/01/22 06:00 [entrez] PHST- 2014/01/22 06:00 [pubmed] PHST- 2014/11/05 06:00 [medline] AID - 10.1111/nph.12681 [doi] PST - ppublish SO - New Phytol. 2014 Apr;202(2):582-593. doi: 10.1111/nph.12681. Epub 2014 Jan 21. ##### PUB RECORD ##### ## 10.1073/pnas.1820468116 30782811 Zhao, Liu et al., 2019 "Zhao Y, Liu R, Xu Y, Wang M, Zhang J, Bai M, Han C, Xiang F, Wang ZY, Mysore KS, Wen J, Zhou C. AGLF provides C-function in floral organ identity through transcriptional regulation of AGAMOUS in Medicago truncatula. Proc Natl Acad Sci U S A. 2019 Mar 12;116(11):5176-5181. doi: 10.1073/pnas.1820468116. Epub 2019 Feb 19. PMID: 30782811; PMCID: PMC6421450." ## PMID- 30782811 OWN - NLM STAT- MEDLINE DCOM- 20190506 LR - 20200309 IS - 1091-6490 (Electronic) IS - 0027-8424 (Print) IS - 0027-8424 (Linking) VI - 116 IP - 11 DP - 2019 Mar 12 TI - AGLF provides C-function in floral organ identity through transcriptional regulation of AGAMOUS in Medicago truncatula. PG - 5176-5181 LID - 10.1073/pnas.1820468116 [doi] AB - Floral development is one of the model systems for investigating the mechanisms underlying organogenesis in plants. Floral organ identity is controlled by the well-known ABC model, which has been generalized to many flowering plants. Here, we report a previously uncharacterized MYB-like gene, AGAMOUS-LIKE FLOWER (AGLF), involved in flower development in the model legume Medicago truncatula Loss-of-function of AGLF results in flowers with stamens and carpel transformed into extra whorls of petals and sepals. Compared with the loss-of-function mutant of the class C gene AGAMOUS (MtAG) in M. truncatula, the defects in floral organ identity are similar between aglf and mtag, but the floral indeterminacy is enhanced in the aglf mutant. Knockout of AGLF in the mutants of the class A gene MtAP1 or the class B gene MtPI leads to an addition of a loss-of-C-function phenotype, reflecting a conventional relationship of AGLF with the canonical A and B genes. Furthermore, we demonstrate that AGLF activates MtAG in transcriptional levels in control of floral organ identity. These data shed light on the conserved and diverged molecular mechanisms that control flower development and morphology among plant species. FAU - Zhao, Yang AU - Zhao Y AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China. FAU - Liu, Rong AU - Liu R AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China. FAU - Xu, Yiteng AU - Xu Y AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China. FAU - Wang, Minmin AU - Wang M AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China. FAU - Zhang, Jing AU - Zhang J AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China. FAU - Bai, Mingyi AU - Bai M AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China. FAU - Han, Chao AU - Han C AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China. FAU - Xiang, Fengning AU - Xiang F AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China. FAU - Wang, Zeng-Yu AU - Wang ZY AD - Noble Research Institute, LLC, Ardmore, OK 73401. AD - Grassland Agri-Husbandry Research Center, Qingdao Agricultural University, Qingdao 266109, People's Republic China. FAU - Mysore, Kirankumar S AU - Mysore KS AUID- ORCID: 0000-0002-9805-5741 AD - Noble Research Institute, LLC, Ardmore, OK 73401. FAU - Wen, Jiangqi AU - Wen J AD - Noble Research Institute, LLC, Ardmore, OK 73401. FAU - Zhou, Chuanen AU - Zhou C AUID- ORCID: 0000-0001-5667-0255 AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, People's Republic China; czhou@sdu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20190219 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Plant Proteins) SB - IM MH - Flowers/*genetics/growth & development/ultrastructure MH - *Gene Expression Regulation, Plant MH - Medicago truncatula/*genetics/ultrastructure MH - Mutation/genetics MH - Organ Specificity/*genetics MH - Phenotype MH - Plant Proteins/*genetics/metabolism MH - *Transcription, Genetic PMC - PMC6421450 OTO - NOTNLM OT - ABC model OT - AGAMOUS OT - Medicago truncatula OT - flower development COIS- The authors declare no conflict of interest. EDAT- 2019/02/21 06:00 MHDA- 2019/05/07 06:00 PMCR- 2019/08/19 CRDT- 2019/02/21 06:00 PHST- 2019/02/21 06:00 [pubmed] PHST- 2019/05/07 06:00 [medline] PHST- 2019/02/21 06:00 [entrez] PHST- 2019/08/19 00:00 [pmc-release] AID - 1820468116 [pii] AID - 201820468 [pii] AID - 10.1073/pnas.1820468116 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2019 Mar 12;116(11):5176-5181. doi: 10.1073/pnas.1820468116. Epub 2019 Feb 19. ##### PUB RECORD ##### ## 10.1111/j.1365-313X.2010.04474.x 21276104 Krusell, Sato et al., 2010 "Krusell L, Sato N, Fukuhara I, Koch BE, Grossmann C, Okamoto S, Oka-Kira E, Otsubo Y, Aubert G, Nakagawa T, Sato S, Tabata S, Duc G, Parniske M, Wang TL, Kawaguchi M, Stougaard J. The Clavata2 genes of pea and Lotus japonicus affect autoregulation of nodulation. Plant J. 2011 Mar;65(6):861-71. doi: 10.1111/j.1365-313X.2010.04474.x. Epub 2011 Jan 31. PMID: 21276104." ## PMID- 21276104 OWN - NLM STAT- MEDLINE DCOM- 20110721 LR - 20240109 IS - 1365-313X (Electronic) IS - 0960-7412 (Linking) VI - 65 IP - 6 DP - 2011 Mar TI - The Clavata2 genes of pea and Lotus japonicus affect autoregulation of nodulation. PG - 861-71 LID - 10.1111/j.1365-313X.2010.04474.x [doi] AB - The number of root nodules developing on legume roots after rhizobial infection is controlled by the plant shoot through autoregulation and mutational inactivation of this mechanism leads to hypernodulation. We have characterised the Pisum sativum (pea) Sym28 locus involved in autoregulation and shown that it encodes a protein similar to the Arabidopsis CLAVATA2 (CLV2) protein. Inactivation of the PsClv2 gene in four independent sym28 mutant alleles, carrying premature stop codons, results in hypernodulation of the root and changes to the shoot architecture. In the reproductive phase sym28 shoots develops additional flowers, the stem fasciates, and the normal phyllotaxis is perturbed. Mutational substitution of an amino acid in one leucine rich repeat of the corresponding Lotus japonicus LjCLV2 protein results in increased nodulation. Similarly, down-regulation of the Lotus Clv2 gene by RNAi mediated reduction of the transcript level also resulted in increased nodulation. Gene expression analysis of LjClv2 and Lotus hypernodulation aberrant root formation Har1 (previously shown to regulate nodule numbers) indicated they have overlapping organ expression patterns. However, we were unable to demonstrate a direct protein-protein interaction between LjCLV2 and LjHAR1 proteins in contrast to the situation between equivalent proteins in Arabidopsis. LjHAR1 was localised to the plasma membrane using a YFP fusion whereas LjCLV2-YFP localised to the endoplasmic reticulum when transiently expressed in Nicotiana benthamiana leaves. This finding is the most likely explanation for the lack of interaction between these two proteins. CI - (c) 2011 The Authors. The Plant Journal (c) 2011 Blackwell Publishing Ltd. FAU - Krusell, Lene AU - Krusell L AD - Centre for Carbohydrate Recognition and Signalling, Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark. FAU - Sato, Naoto AU - Sato N FAU - Fukuhara, Izumi AU - Fukuhara I FAU - Koch, Bjorn E V AU - Koch BE FAU - Grossmann, Christina AU - Grossmann C FAU - Okamoto, Satoru AU - Okamoto S FAU - Oka-Kira, Erika AU - Oka-Kira E FAU - Otsubo, Yoko AU - Otsubo Y FAU - Aubert, Gregoire AU - Aubert G FAU - Nakagawa, Tomomi AU - Nakagawa T FAU - Sato, Shusei AU - Sato S FAU - Tabata, Satoshi AU - Tabata S FAU - Duc, Gerard AU - Duc G FAU - Parniske, Martin AU - Parniske M FAU - Wang, Trevor L AU - Wang TL FAU - Kawaguchi, Masayoshi AU - Kawaguchi M FAU - Stougaard, Jens AU - Stougaard J LA - eng GR - BBS/E/J/00000150/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom GR - BBS/B/02401/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20110131 PL - England TA - Plant J JT - The Plant journal : for cell and molecular biology JID - 9207397 RN - 0 (DNA, Plant) RN - 0 (Plant Proteins) SB - IM MH - Amino Acid Sequence MH - Amino Acid Substitution MH - Base Sequence MH - DNA, Plant/genetics MH - *Genes, Plant MH - Homeostasis/genetics/physiology MH - Lotus/*genetics/growth & development/*physiology MH - Models, Biological MH - Molecular Sequence Data MH - Mutagenesis, Site-Directed MH - Pisum sativum/*genetics/growth & development/*physiology MH - Phenotype MH - Plant Proteins/genetics/physiology MH - Plant Root Nodulation/*genetics/*physiology MH - Plants, Genetically Modified MH - RNA Interference MH - Sequence Homology, Amino Acid MH - Species Specificity MH - Nicotiana/genetics/physiology EDAT- 2011/02/01 06:00 MHDA- 2011/07/22 06:00 CRDT- 2011/02/01 06:00 PHST- 2011/02/01 06:00 [entrez] PHST- 2011/02/01 06:00 [pubmed] PHST- 2011/07/22 06:00 [medline] AID - 10.1111/j.1365-313X.2010.04474.x [doi] PST - ppublish SO - Plant J. 2011 Mar;65(6):861-71. doi: 10.1111/j.1365-313X.2010.04474.x. Epub 2011 Jan 31. ##### PUB RECORD ##### ## 10.1104/pp.108.125062 18790999 Floss, Schliemann et al., 2008 "Floss DS, Schliemann W, Schmidt J, Strack D, Walter MH. RNA interference-mediated repression of MtCCD1 in mycorrhizal roots of Medicago truncatula causes accumulation of C27 apocarotenoids, shedding light on the functional role of CCD1. Plant Physiol. 2008 Nov;148(3):1267-82. doi: 10.1104/pp.108.125062. Epub 2008 Sep 12. PMID: 18790999; PMCID: PMC2577242." ## PMID- 18790999 OWN - NLM STAT- MEDLINE DCOM- 20090102 LR - 20211020 IS - 0032-0889 (Print) IS - 1532-2548 (Electronic) IS - 0032-0889 (Linking) VI - 148 IP - 3 DP - 2008 Nov TI - RNA interference-mediated repression of MtCCD1 in mycorrhizal roots of Medicago truncatula causes accumulation of C27 apocarotenoids, shedding light on the functional role of CCD1. PG - 1267-82 LID - 10.1104/pp.108.125062 [doi] AB - Tailoring carotenoids by plant carotenoid cleavage dioxygenases (CCDs) generates various bioactive apocarotenoids. Recombinant CCD1 has been shown to catalyze symmetrical cleavage of C(40) carotenoid substrates at 9,10 and 9',10' positions. The actual substrate(s) of the enzyme in planta, however, is still unknown. In this study, we have carried out RNA interference (RNAi)-mediated repression of a Medicago truncatula CCD1 gene in hairy roots colonized by the arbuscular mycorrhizal (AM) fungus Glomus intraradices. As a consequence, the normal AM-mediated accumulation of apocarotenoids (C(13) cyclohexenone and C(14) mycorradicin derivatives) was differentially modified. Mycorradicin derivatives were strongly reduced to 3% to 6% of the controls, while the cyclohexenone derivatives were only reduced to 30% to 47%. Concomitantly, a yellow-orange color appeared in RNAi roots. Based on ultraviolet light spectra and mass spectrometry analyses, the new compounds are C(27) apocarotenoic acid derivatives. These metabolic alterations did not lead to major changes in molecular markers of the AM symbiosis, although a moderate shift to more degenerating arbuscules was observed in RNAi roots. The unexpected outcome of the RNAi approach suggests C(27) apocarotenoids as the major substrates of CCD1 in mycorrhizal root cells. Moreover, literature data implicate C(27) apocarotenoid cleavage as the general functional role of CCD1 in planta. A revised scheme of plant carotenoid cleavage in two consecutive steps is proposed, in which CCD1 catalyzes only the second step in the cytosol (C(27)-->C(14)+C(13)), while the first step (C(40)-->C(27)+C(13)) may be catalyzed by CCD7 and/or CCD4 inside plastids. FAU - Floss, Daniela S AU - Floss DS AD - Leibniz-Institut fur Pflanzenbiochemie, Abteilung Sekundarstoffwechsel , D-06120 Halle, Germany. FAU - Schliemann, Willibald AU - Schliemann W FAU - Schmidt, Jurgen AU - Schmidt J FAU - Strack, Dieter AU - Strack D FAU - Walter, Michael H AU - Walter MH LA - eng SI - GENBANK/FM204879 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080912 PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 RN - 0 (DNA Primers) RN - 0 (DNA, Complementary) RN - 0 (RNA, Messenger) RN - 36-88-4 (Carotenoids) RN - EC 1.13.11.- (Dioxygenases) RN - EC 1.13.11.- (carotenoid cleavage dioxygenase 1) SB - IM MH - Base Sequence MH - Carotenoids/*metabolism MH - Chromatography, High Pressure Liquid MH - Cloning, Molecular MH - DNA Primers MH - DNA, Complementary MH - Dioxygenases/*genetics/metabolism MH - *Genes, Plant MH - Mass Spectrometry MH - Medicago truncatula/genetics/*metabolism MH - Molecular Sequence Data MH - Plant Roots/*enzymology MH - *RNA Interference MH - RNA, Messenger/genetics MH - Reverse Transcriptase Polymerase Chain Reaction PMC - PMC2577242 EDAT- 2008/09/16 09:00 MHDA- 2009/01/03 09:00 PMCR- 2008/11/01 CRDT- 2008/09/16 09:00 PHST- 2008/09/16 09:00 [pubmed] PHST- 2009/01/03 09:00 [medline] PHST- 2008/09/16 09:00 [entrez] PHST- 2008/11/01 00:00 [pmc-release] AID - pp.108.125062 [pii] AID - 125062 [pii] AID - 10.1104/pp.108.125062 [doi] PST - ppublish SO - Plant Physiol. 2008 Nov;148(3):1267-82. doi: 10.1104/pp.108.125062. Epub 2008 Sep 12. ##### PUB RECORD ##### ## 10.1093/plphys/kiaa005 33631796 Cheng, Li et al., 2021 "Cheng X, Li G, Krom N, Tang Y, Wen J. Genetic regulation of flowering time and inflorescence architecture by MtFDa and MtFTa1 in Medicago truncatula. Plant Physiol. 2021 Feb 25;185(1):161-178. doi: 10.1093/plphys/kiaa005. PMID: 33631796; PMCID: PMC8133602." ## PMID- 33631796 OWN - NLM STAT- MEDLINE DCOM- 20210705 LR - 20231111 IS - 1532-2548 (Electronic) IS - 0032-0889 (Print) IS - 0032-0889 (Linking) VI - 185 IP - 1 DP - 2021 Feb 25 TI - Genetic regulation of flowering time and inflorescence architecture by MtFDa and MtFTa1 in Medicago truncatula. PG - 161-178 LID - 10.1093/plphys/kiaa005 [doi] AB - Regulation of floral transition and inflorescence development is crucial for plant reproductive success. FLOWERING LOCUS T (FT) is one of the central players in the flowering genetic regulatory network, whereas FLOWERING LOCUS D (FD), an interactor of FT and TERMINAL FLOWER 1 (TFL1), plays significant roles in both floral transition and inflorescence development. Here we show the genetic regulatory networks of floral transition and inflorescence development in Medicago truncatula by characterizing MtFTa1 and MtFDa and their genetic interactions with key inflorescence meristem (IM) regulators. Both MtFTa1 and MtFDa promote flowering; the double mutant mtfda mtfta1 does not proceed to floral transition. RNAseq analysis reveals that a broad range of genes involved in flowering regulation and flower development are up- or downregulated by MtFTa1 and/or MtFDa mutations. Furthermore, mutation of MtFDa also affects the inflorescence architecture. Genetic analyses of MtFDa, MtFTa1, MtTFL1, and MtFULc show that MtFDa is epistatic to MtFULc and MtTFL1 in controlling IM identity. Our results demonstrate that MtFTa1 and MtFDa are major flowering regulators in M. truncatula, and MtFDa is essential both in floral transition and secondary inflorescence development. The study will advance our understanding of the genetic regulation of flowering time and inflorescence development in legumes. CI - (c) The Author(s) 2020. Published by Oxford University Press on behalf of American Society of Plant Biologists. FAU - Cheng, Xiaofei AU - Cheng X AD - Noble Research Institute, Ardmore, Oklahoma 73401, USA. FAU - Li, Guifen AU - Li G AD - Noble Research Institute, Ardmore, Oklahoma 73401, USA. FAU - Krom, Nick AU - Krom N AD - Noble Research Institute, Ardmore, Oklahoma 73401, USA. FAU - Tang, Yuhong AU - Tang Y AD - Noble Research Institute, Ardmore, Oklahoma 73401, USA. FAU - Wen, Jiangqi AU - Wen J AD - Noble Research Institute, Ardmore, Oklahoma 73401, USA. LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 SB - IM MH - Gene Expression Regulation, Developmental MH - Gene Expression Regulation, Plant MH - Gene Regulatory Networks MH - Genes, Plant MH - Genetic Variation MH - Genotype MH - Inflorescence/*anatomy & histology/*genetics/*growth & development MH - Magnoliopsida/*genetics/*growth & development MH - Medicago truncatula/*genetics/*growth & development MH - Mutation MH - Phenotype MH - Plants, Genetically Modified PMC - PMC8133602 EDAT- 2021/02/26 06:00 MHDA- 2021/07/06 06:00 PMCR- 2020/11/17 CRDT- 2021/02/25 20:19 PHST- 2020/08/03 00:00 [received] PHST- 2020/10/11 00:00 [accepted] PHST- 2021/02/25 20:19 [entrez] PHST- 2021/02/26 06:00 [pubmed] PHST- 2021/07/06 06:00 [medline] PHST- 2020/11/17 00:00 [pmc-release] AID - 5985539 [pii] AID - kiaa005 [pii] AID - 10.1093/plphys/kiaa005 [doi] PST - ppublish SO - Plant Physiol. 2021 Feb 25;185(1):161-178. doi: 10.1093/plphys/kiaa005. ##### PUB RECORD ##### ## 10.1073/pnas.0400595101 15070781 Mitra, Gleason et al., 2004 "Mitra RM, Gleason CA, Edwards A, Hadfield J, Downie JA, Oldroyd GE, Long SR. A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: Gene identification by transcript-based cloning. Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4701-5. doi: 10.1073/pnas.0400595101. Epub 2004 Mar 1. PMID: 15070781; PMCID: PMC384810." ## PMID- 15070781 OWN - NLM STAT- MEDLINE DCOM- 20040719 LR - 20241122 IS - 0027-8424 (Print) IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 101 IP - 13 DP - 2004 Mar 30 TI - A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: Gene identification by transcript-based cloning. PG - 4701-5 AB - In the establishment of the legume-rhizobial symbiosis, bacterial lipochitooligosaccharide signaling molecules termed Nod factors activate the formation of a novel root organ, the nodule. Nod factors elicit several responses in plant root hair cells, including oscillations in cytoplasmic calcium levels (termed calcium spiking) and alterations in root hair growth. A number of plant mutants with defects in the Nod factor signaling pathway have been identified. One such Medicago truncatula mutant, dmi3, exhibits calcium spiking and root hair swelling in response to Nod factor, but fails to initiate symbiotic gene expression or cell divisions for nodule formation. On the basis of these data, it is thought that the dmi3 mutant perceives Nod factor but fails to transduce the signal downstream of calcium spiking. Additionally, the dmi3 mutant is defective in the symbiosis with mycorrhizal fungi, indicating the importance of the encoded protein in multiple symbioses. We report the identification of the DMI3 gene, using a gene cloning method based on transcript abundance. We show that transcript-based cloning is a valid approach for cloning genes in barley, indicating the value of this technology in crop plants. DMI3 encodes a calcium/calmodulin-dependent protein kinase. Mutants in pea sym9 have phenotypes similar to dmi3 and have alterations in this gene. The DMI3 class of proteins is well conserved among plants that interact with mycorrhizal fungi, but it is less conserved in Arabidopsis thaliana, which does not participate in the mycorrhizal symbiosis. FAU - Mitra, Raka M AU - Mitra RM AD - Department of Biological Sciences, 371 Serra Mall, Stanford University, Stanford, CA 94305-5020, USA. FAU - Gleason, Cynthia A AU - Gleason CA FAU - Edwards, Anne AU - Edwards A FAU - Hadfield, James AU - Hadfield J FAU - Downie, J Allan AU - Downie JA FAU - Oldroyd, Giles E D AU - Oldroyd GE FAU - Long, Sharon R AU - Long SR LA - eng SI - GENBANK/AJ621916 SI - GENBANK/AY496049 PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20040301 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (DNA Primers) RN - 0 (Recombinant Proteins) RN - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases) SB - IM CIN - Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4339-40. doi: 10.1073/pnas.0400961101. PMID: 15070718 MH - Amino Acid Sequence MH - Base Sequence MH - Calcium-Calmodulin-Dependent Protein Kinases/*genetics/*metabolism MH - Cloning, Molecular MH - Conserved Sequence MH - DNA Primers MH - Hordeum/enzymology/physiology MH - Medicago/*enzymology/*physiology MH - Molecular Sequence Data MH - Mycorrhizae/physiology MH - Recombinant Proteins/metabolism MH - Reverse Transcriptase Polymerase Chain Reaction MH - Sequence Alignment MH - Sequence Homology, Amino Acid MH - Symbiosis MH - Transcription, Genetic PMC - PMC384810 EDAT- 2004/04/09 05:00 MHDA- 2004/07/20 05:00 PMCR- 2004/09/30 CRDT- 2004/04/09 05:00 PHST- 2004/04/09 05:00 [pubmed] PHST- 2004/07/20 05:00 [medline] PHST- 2004/04/09 05:00 [entrez] PHST- 2004/09/30 00:00 [pmc-release] AID - 0400595101 [pii] AID - 1014701 [pii] AID - 10.1073/pnas.0400595101 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4701-5. doi: 10.1073/pnas.0400595101. Epub 2004 Mar 1. ##### PUB RECORD ##### ## 10.1186/1471-2229-10-183 20723225 Seabra, Vieria et al., 2010 "Seabra AR, Vieira CP, Cullimore JV, Carvalho HG. Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds. BMC Plant Biol. 2010 Aug 19;10:183. doi: 10.1186/1471-2229-10-183. PMID: 20723225; PMCID: PMC3095313." ## PMID- 20723225 OWN - NLM STAT- MEDLINE DCOM- 20101006 LR - 20211020 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 10 DP - 2010 Aug 19 TI - Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds. PG - 183 LID - 10.1186/1471-2229-10-183 [doi] AB - BACKGROUND: Nitrogen is a crucial nutrient that is both essential and rate limiting for plant growth and seed production. Glutamine synthetase (GS), occupies a central position in nitrogen assimilation and recycling, justifying the extensive number of studies that have been dedicated to this enzyme from several plant sources. All plants species studied to date have been reported as containing a single, nuclear gene encoding a plastid located GS isoenzyme per haploid genome. This study reports the existence of a second nuclear gene encoding a plastid located GS in Medicago truncatula. RESULTS: This study characterizes a new, second gene encoding a plastid located glutamine synthetase (GS2) in M. truncatula. The gene encodes a functional GS isoenzyme with unique kinetic properties, which is exclusively expressed in developing seeds. Based on molecular data and the assumption of a molecular clock, it is estimated that the gene arose from a duplication event that occurred about 10 My ago, after legume speciation and that duplicated sequences are also present in closely related species of the Vicioide subclade. Expression analysis by RT-PCR and western blot indicate that the gene is exclusively expressed in developing seeds and its expression is related to seed filling, suggesting a specific function of the enzyme associated to legume seed metabolism. Interestingly, the gene was found to be subjected to alternative splicing over the first intron, leading to the formation of two transcripts with similar open reading frames but varying 5' UTR lengths, due to retention of the first intron. To our knowledge, this is the first report of alternative splicing on a plant GS gene. CONCLUSIONS: This study shows that Medicago truncatula contains an additional GS gene encoding a plastid located isoenzyme, which is functional and exclusively expressed during seed development. Legumes produce protein-rich seeds requiring high amounts of nitrogen, we postulate that this gene duplication represents a functional innovation of plastid located GS related to storage protein accumulation exclusive to legume seed metabolism. FAU - Seabra, Ana R AU - Seabra AR AD - Instituto de Biologia Molecular e Celular da Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal. FAU - Vieira, Cristina P AU - Vieira CP FAU - Cullimore, Julie V AU - Cullimore JV FAU - Carvalho, Helena G AU - Carvalho HG LA - eng SI - GENBANK/HM775420 SI - GENBANK/HM775421 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20100819 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 RN - EC 6.3.1.2 (Glutamate-Ammonia Ligase) SB - IM MH - Amino Acid Sequence MH - Gene Expression Profiling MH - *Gene Expression Regulation, Plant MH - Glutamate-Ammonia Ligase/*genetics/*metabolism MH - Medicago truncatula/classification/*enzymology/*genetics/growth & development MH - Molecular Sequence Data MH - Mutation MH - Plastids/*enzymology MH - Seeds/*enzymology/growth & development MH - Sequence Alignment PMC - PMC3095313 EDAT- 2010/08/21 06:00 MHDA- 2010/10/07 06:00 PMCR- 2010/08/19 CRDT- 2010/08/21 06:00 PHST- 2010/04/05 00:00 [received] PHST- 2010/08/19 00:00 [accepted] PHST- 2010/08/21 06:00 [entrez] PHST- 2010/08/21 06:00 [pubmed] PHST- 2010/10/07 06:00 [medline] PHST- 2010/08/19 00:00 [pmc-release] AID - 1471-2229-10-183 [pii] AID - 10.1186/1471-2229-10-183 [doi] PST - epublish SO - BMC Plant Biol. 2010 Aug 19;10:183. doi: 10.1186/1471-2229-10-183. ##### PUB RECORD ##### ## 10.3390/plants13071012 38611541 Su, Zheng etal., 2024 "Su X, Zheng J, Diao X, Yang Z, Yu D, Huang F. MtTCP18 Regulates Plant Structure in Medicago truncatula. Plants (Basel). 2024 Apr 2;13(7):1012. doi: 10.3390/plants13071012. PMID: 38611541; PMCID: PMC11013128." ## PMID- 38611541 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240415 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 13 IP - 7 DP - 2024 Apr 2 TI - MtTCP18 Regulates Plant Structure in Medicago truncatula. LID - 10.3390/plants13071012 [doi] LID - 1012 AB - Plant structure has a large influence on crop yield formation, with branching and plant height being the important factors that make it up. We identified a gene, MtTCP18, encoding a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor highly conserved with Arabidopsis gene BRC1 (BRANCHED1) in Medicago truncatula. Sequence analysis revealed that MtTCP18 included a conserved basic helix-loop-helix (BHLH) motif and R domain. Expression analysis showed that MtTCP18 was expressed in all organs examined, with relatively higher expression in pods and axillary buds. Subcellular localization analysis showed that MtTCP18 was localized in the nucleus and exhibited transcriptional activation activity. These results supported its role as a transcription factor. Meanwhile, we identified a homozygous mutant line (NF14875) with a mutation caused by Tnt1 insertion into MtTCP18. Mutant analysis showed that the mutation of MtTCP18 altered plant structure, with increased plant height and branch number. Moreover, we found that the expression of auxin early response genes was modulated in the mutant. Therefore, MtTCP18 may be a promising candidate gene for breeders to optimize plant structure for crop improvement. FAU - Su, Xiaoyue AU - Su X AD - Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China. FAU - Zheng, Junzan AU - Zheng J AD - Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China. FAU - Diao, Xiaoxuan AU - Diao X AD - Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China. FAU - Yang, Zhongyi AU - Yang Z AD - Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China. FAU - Yu, Deyue AU - Yu D AD - Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China. FAU - Huang, Fang AU - Huang F AD - Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China. LA - eng PT - Journal Article DEP - 20240402 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC11013128 OTO - NOTNLM OT - Medicago truncatula OT - TCP transcription factors OT - Tnt1 mutant OT - auxin OT - plant structure COIS- The authors declare no competing interests. EDAT- 2024/04/13 10:43 MHDA- 2024/04/13 10:44 PMCR- 2024/04/02 CRDT- 2024/04/13 01:08 PHST- 2024/02/18 00:00 [received] PHST- 2024/03/18 00:00 [revised] PHST- 2024/03/25 00:00 [accepted] PHST- 2024/04/13 10:44 [medline] PHST- 2024/04/13 10:43 [pubmed] PHST- 2024/04/13 01:08 [entrez] PHST- 2024/04/02 00:00 [pmc-release] AID - plants13071012 [pii] AID - plants-13-01012 [pii] AID - 10.3390/plants13071012 [doi] PST - epublish SO - Plants (Basel). 2024 Apr 2;13(7):1012. doi: 10.3390/plants13071012. ##### PUB RECORD ##### ## 10.1111/ppl.14046 37882293 Mao, Zhou et al., 2023 "Mao Y, Zhou S, Yang J, Wen J, Wang D, Zhou X, Wu X, He L, Liu M, Wu H, Yang L, Zhao B, Tadege M, Liu Y, Liu C, Chen J. The MIO1-MtKIX8 module regulates the organ size in Medicago truncatula. Physiol Plant. 2023 Sep-Oct;175(5):e14046. doi: 10.1111/ppl.14046. PMID: 37882293." ## PMID- 37882293 OWN - NLM STAT- MEDLINE DCOM- 20231102 LR - 20231102 IS - 1399-3054 (Electronic) IS - 0031-9317 (Linking) VI - 175 IP - 5 DP - 2023 Sep-Oct TI - The MIO1-MtKIX8 module regulates the organ size in Medicago truncatula. PG - e14046 LID - 10.1111/ppl.14046 [doi] AB - Plant organ size is an important agronomic trait tightly related to crop yield. However, the molecular mechanisms underlying organ size regulation remain largely unexplored in legumes. We previously characterized a key regulator F-box protein MINI ORGAN1 (MIO1)/SMALL LEAF AND BUSHY1 (SLB1), which controls plant organ size in the model legume Medicago truncatula. In order to further dissect the molecular mechanism, MIO1 was used as the bait to screen its interacting proteins from a yeast library. Subsequently, a KIX protein, designated MtKIX8, was identified from the candidate list. The interaction between MIO1 and MtKIX8 was confirmed further by Y2H, BiFC, split-luciferase complementation and pull-down assays. Phylogenetic analyses indicated that MtKIX8 is highly homologous to Arabidopsis KIX8, which negatively regulates organ size. Moreover, loss-of-function of MtKIX8 led to enlarged leaves and seeds, while ectopic expression of MtKIX8 in Arabidopsis resulted in decreased cotyledon area and seed weight. Quantitative reverse-transcription PCR and in situ hybridization showed that MtKIX8 is expressed in most developing organs. We also found that MtKIX8 serves as a crucial molecular adaptor, facilitating interactions with BIG SEEDS1 (BS1) and MtTOPLESS (MtTPL) proteins in M. truncatula. Overall, our results suggest that the MIO1-MtKIX8 module plays a significant and conserved role in the regulation of plant organ size. This module could be a good target for molecular breeding in legume crops and forages. CI - (c) 2023 Scandinavian Plant Physiology Society. FAU - Mao, Yawen AU - Mao Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Zhou, Shaoli AU - Zhou S AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Yang, Jing AU - Yang J AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - School of Ecology and Environmental Science, Yunnan University, Kunming, China. FAU - Wen, Jiangqi AU - Wen J AD - Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, Oklahoma, USA. FAU - Wang, Dongfa AU - Wang D AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - School of Life Sciences, University of Science and Technology of China, Hefei, China. FAU - Zhou, Xuan AU - Zhou X AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Wu, Xinyuan AU - Wu X AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - He, Liangliang AU - He L AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. FAU - Liu, Mingli AU - Liu M AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - Southwest Forestry University, Kunming, China. FAU - Wu, Huan AU - Wu H AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - School of Life Sciences, University of Science and Technology of China, Hefei, China. FAU - Yang, Liling AU - Yang L AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. FAU - Zhao, Baolin AU - Zhao B AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. FAU - Tadege, Million AU - Tadege M AD - Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, Oklahoma, USA. FAU - Liu, Yu AU - Liu Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. FAU - Liu, Changning AU - Liu C AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. FAU - Chen, Jianghua AU - Chen J AUID- ORCID: 0000-0003-0715-1859 AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, China. AD - University of Chinese Academy of Sciences, Beijing, China. AD - School of Ecology and Environmental Science, Yunnan University, Kunming, China. LA - eng GR - XDA26030301/Strategic Priority Research Program of Chinese Academy of Sciences/ GR - XDB27030106/Strategic Priority Research Program of Chinese Academy of Sciences/ GR - xbzg-zdsys-202016/Cross-Team Project-Key Laboratory Cooperative Research Project/ GR - 2015HA031/High-end Scientific and Technological Talents in Yunnan Province/ GR - 2015HA032/High-end Scientific and Technological Talents in Yunnan Province/ GR - 32170839/National Natural Science Foundation of China/ GR - 32200290/National Natural Science Foundation of China/ GR - 2021395/Youth Innovation Promotion Association CAS/ GR - E3YN103K01/Yunnan Revitalization Talent Support Program/ GR - XDYC-QNRC-2022-0179/Yunnan Revitalization Talent Support Program/ PT - Journal Article PL - Denmark TA - Physiol Plant JT - Physiologia plantarum JID - 1256322 RN - 0 (Plant Proteins) RN - 0 (KIX8 protein, Arabidopsis) RN - 0 (Arabidopsis Proteins) RN - 0 (Adaptor Proteins, Signal Transducing) SB - IM MH - *Medicago truncatula/genetics MH - Plant Proteins/metabolism MH - *Arabidopsis/genetics/metabolism MH - Organ Size MH - Phylogeny MH - Gene Expression Regulation, Plant MH - *Arabidopsis Proteins/metabolism MH - Adaptor Proteins, Signal Transducing/metabolism EDAT- 2023/10/26 12:42 MHDA- 2023/10/26 12:43 CRDT- 2023/10/26 06:51 PHST- 2023/09/19 00:00 [revised] PHST- 2023/08/16 00:00 [received] PHST- 2023/10/04 00:00 [accepted] PHST- 2023/10/26 12:43 [medline] PHST- 2023/10/26 12:42 [pubmed] PHST- 2023/10/26 06:51 [entrez] AID - 10.1111/ppl.14046 [doi] PST - ppublish SO - Physiol Plant. 2023 Sep-Oct;175(5):e14046. doi: 10.1111/ppl.14046. ##### PUB RECORD ##### ## 10.1073/pnas.2205920119 35972963 Liu, Lin et al., 2022 "Liu H, Lin JS, Luo Z, Sun J, Huang X, Yang Y, Xu J, Wang YF, Zhang P, Oldroyd GED, Xie F. Constitutive activation of a nuclear-localized calcium channel complex in Medicago truncatula. Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2205920119. doi: 10.1073/pnas.2205920119. Epub 2022 Aug 16. PMID: 35972963; PMCID: PMC9407390." ## PMID- 35972963 OWN - NLM STAT- MEDLINE DCOM- 20220818 LR - 20230217 IS - 1091-6490 (Electronic) IS - 0027-8424 (Print) IS - 0027-8424 (Linking) VI - 119 IP - 34 DP - 2022 Aug 23 TI - Constitutive activation of a nuclear-localized calcium channel complex in Medicago truncatula. PG - e2205920119 LID - 10.1073/pnas.2205920119 [doi] LID - e2205920119 AB - Nuclear Ca(2+) oscillations allow symbiosis signaling, facilitating plant recognition of beneficial microsymbionts, nitrogen-fixing rhizobia, and nutrient-capturing arbuscular mycorrhizal fungi. Two classes of channels, DMI1 and CNGC15, in a complex on the nuclear membrane, coordinate symbiotic Ca(2+) oscillations. However, the mechanism of Ca(2+) signature generation is unknown. Here, we demonstrate spontaneous activation of this channel complex, through gain-of-function mutations in DMI1, leading to spontaneous nuclear Ca(2+) oscillations and spontaneous nodulation, in a CNGC15-dependent manner. The mutations destabilize a hydrogen-bond or salt-bridge network between two RCK domains, with the resultant structural changes, alongside DMI1 cation permeability, activating the channel complex. This channel complex was reconstituted in human HEK293T cell lines, with the resultant calcium influx enhanced by autoactivated DMI1 and CNGC15s. Our results demonstrate the mode of activation of this nuclear channel complex, show that DMI1 and CNGC15 are sufficient to create oscillatory Ca(2+) signals, and provide insights into its native mode of induction. FAU - Liu, Haiyue AU - Liu H AUID- ORCID: 0000-0001-9009-2153 AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. AD - University of the Chinese Academy of Sciences, Beijing, 100049, China. FAU - Lin, Jie-Shun AU - Lin JS AUID- ORCID: 0000-0002-3726-0303 AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. AD - University of the Chinese Academy of Sciences, Beijing, 100049, China. FAU - Luo, Zhenpeng AU - Luo Z AUID- ORCID: 0000-0002-4512-8505 AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. AD - University of the Chinese Academy of Sciences, Beijing, 100049, China. FAU - Sun, Jongho AU - Sun J AUID- ORCID: 0000-0002-3705-3072 AD - Sainsbury Laboratory, University of Cambridge, 47 Bateman Street, Cambridge, CB2 1LR, United Kingdom. FAU - Huang, Xiaowei AU - Huang X AUID- ORCID: 0000-0001-9964-687X AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. AD - University of the Chinese Academy of Sciences, Beijing, 100049, China. FAU - Yang, Yang AU - Yang Y AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. AD - University of the Chinese Academy of Sciences, Beijing, 100049, China. FAU - Xu, Ji AU - Xu J AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. FAU - Wang, Yong-Fei AU - Wang YF AUID- ORCID: 0000-0003-3139-7701 AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. FAU - Zhang, Peng AU - Zhang P AUID- ORCID: 0000-0003-0408-2923 AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. FAU - Oldroyd, Giles E D AU - Oldroyd GED AUID- ORCID: 0000-0002-5245-6355 AD - Sainsbury Laboratory, University of Cambridge, 47 Bateman Street, Cambridge, CB2 1LR, United Kingdom. FAU - Xie, Fang AU - Xie F AUID- ORCID: 0000-0002-0530-1430 AD - National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220816 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Calcium Channels) RN - 0 (Plant Proteins) RN - SY7Q814VUP (Calcium) SB - IM MH - Calcium/metabolism MH - *Calcium Channels/genetics/metabolism MH - *Calcium Signaling/physiology MH - Cell Nucleus/metabolism MH - Gain of Function Mutation MH - Gene Expression Regulation, Plant MH - HEK293 Cells MH - Humans MH - *Medicago truncatula/genetics/physiology MH - *Plant Proteins/genetics/metabolism MH - *Plant Root Nodulation/genetics/physiology MH - *Plant Roots/genetics/physiology MH - Symbiosis/physiology PMC - PMC9407390 OTO - NOTNLM OT - CNGC15 OT - DMI1 OT - NF signaling OT - calcium channel OT - nuclear calcium spiking COIS- The authors declare no competing interest. EDAT- 2022/08/17 06:00 MHDA- 2022/08/19 06:00 PMCR- 2023/02/16 CRDT- 2022/08/16 13:42 PHST- 2022/08/16 13:42 [entrez] PHST- 2022/08/17 06:00 [pubmed] PHST- 2022/08/19 06:00 [medline] PHST- 2023/02/16 00:00 [pmc-release] AID - 202205920 [pii] AID - 10.1073/pnas.2205920119 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2205920119. doi: 10.1073/pnas.2205920119. Epub 2022 Aug 16. ##### PUB RECORD ##### ## 10.1105/tpc.107.053975 18156218 Kevei, Lougnon et al., 2007 "Kevei Z, Lougnon G, Mergaert P, Horváth GV, Kereszt A, Jayaraman D, Zaman N, Marcel F, Regulski K, Kiss GB, Kondorosi A, Endre G, Kondorosi E, Ané JM. 3-hydroxy-3-methylglutaryl coenzyme a reductase 1 interacts with NORK and is crucial for nodulation in Medicago truncatula. Plant Cell. 2007 Dec;19(12):3974-89. doi: 10.1105/tpc.107.053975. Epub 2007 Dec 21. PMID: 18156218; PMCID: PMC2217646." ## PMID- 18156218 OWN - NLM STAT- MEDLINE DCOM- 20080808 LR - 20181113 IS - 1040-4651 (Print) IS - 1532-298X (Electronic) IS - 1040-4651 (Linking) VI - 19 IP - 12 DP - 2007 Dec TI - 3-hydroxy-3-methylglutaryl coenzyme a reductase 1 interacts with NORK and is crucial for nodulation in Medicago truncatula. PG - 3974-89 AB - NORK in legumes encodes a receptor-like kinase that is required for Nod factor signaling and root nodule development. Using Medicago truncatula NORK as bait in a yeast two-hybrid assay, we identified 3-hydroxy-3-methylglutaryl CoA reductase 1 (Mt HMGR1) as a NORK interacting partner. HMGR1 belongs to a multigene family in M. truncatula, and different HMGR isoforms are key enzymes in the mevalonate biosynthetic pathway leading to the production of a diverse array of isoprenoid compounds. Testing other HMGR members revealed a specific interaction between NORK and HMGR1. Mutagenesis and deletion analysis showed that this interaction requires the cytosolic active kinase domain of NORK and the cytosolic catalytic domain of HMGR1. NORK homologs from Lotus japonicus and Sesbania rostrata also interacted with Mt HMGR1, but homologous nonsymbiotic kinases of M. truncatula did not. Pharmacological inhibition of HMGR activities decreased nodule number and delayed nodulation, supporting the importance of the mevalonate pathway in symbiotic development. Decreasing HMGR1 expression in M. truncatula transgenic roots by RNA interference led to a dramatic decrease in nodulation, confirming that HMGR1 is essential for nodule development. Recruitment of HMGR1 by NORK could be required for production of specific isoprenoid compounds, such as cytokinins, phytosteroids, or isoprenoid moieties involved in modification of signaling proteins. FAU - Kevei, Zoltan AU - Kevei Z AD - Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, Unite Propre de Recherche 2355, 91198 Gif-sur-Yvette Cedex, France. FAU - Lougnon, Geraldine AU - Lougnon G FAU - Mergaert, Peter AU - Mergaert P FAU - Horvath, Gabor V AU - Horvath GV FAU - Kereszt, Attila AU - Kereszt A FAU - Jayaraman, Dhileepkumar AU - Jayaraman D FAU - Zaman, Najia AU - Zaman N FAU - Marcel, Fabian AU - Marcel F FAU - Regulski, Krzysztof AU - Regulski K FAU - Kiss, Gyorgy B AU - Kiss GB FAU - Kondorosi, Adam AU - Kondorosi A FAU - Endre, Gabriella AU - Endre G FAU - Kondorosi, Eva AU - Kondorosi E FAU - Ane, Jean-Michel AU - Ane JM LA - eng SI - GENBANK/AF492655 SI - GENBANK/AJ418369 SI - GENBANK/AY751547 SI - GENBANK/EU302813 SI - GENBANK/EU302814 SI - GENBANK/EU302815 SI - GENBANK/EU302816 SI - GENBANK/EU302817 PT - Journal Article DEP - 20071221 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 RN - 0 (Plant Proteins) RN - 0 (Protein Isoforms) RN - 9LHU78OQFD (Lovastatin) RN - EC 1.1.1.- (Hydroxymethylglutaryl CoA Reductases) SB - IM MH - Amino Acid Sequence MH - Enzyme Activation/drug effects MH - Gene Expression Regulation, Plant/drug effects MH - Hydroxymethylglutaryl CoA Reductases/genetics/*metabolism MH - Immunoprecipitation MH - In Situ Hybridization MH - Lovastatin/pharmacology MH - Medicago truncatula/genetics/*metabolism/microbiology MH - Models, Genetic MH - Molecular Sequence Data MH - Mutation MH - Plant Proteins/chemistry/genetics/*metabolism MH - Protein Binding MH - Protein Isoforms/chemistry/genetics/metabolism MH - Protein Structure, Tertiary MH - RNA Interference MH - Reverse Transcriptase Polymerase Chain Reaction MH - Root Nodules, Plant/genetics/*metabolism/microbiology MH - Sequence Homology, Amino Acid MH - Sinorhizobium meliloti/growth & development MH - Symbiosis MH - Two-Hybrid System Techniques PMC - PMC2217646 EDAT- 2007/12/25 09:00 MHDA- 2008/08/09 09:00 PMCR- 2008/12/01 CRDT- 2007/12/25 09:00 PHST- 2007/12/25 09:00 [pubmed] PHST- 2008/08/09 09:00 [medline] PHST- 2007/12/25 09:00 [entrez] PHST- 2008/12/01 00:00 [pmc-release] AID - tpc.107.053975 [pii] AID - 053975 [pii] AID - 10.1105/tpc.107.053975 [doi] PST - ppublish SO - Plant Cell. 2007 Dec;19(12):3974-89. doi: 10.1105/tpc.107.053975. Epub 2007 Dec 21. ##### PUB RECORD ##### ## 10.7554/eLife.80741 36856086 Lace, Su et al., 2023 "Lace B, Su C, Invernot Perez D, Rodriguez-Franco M, Vernié T, Batzenschlager M, Egli S, Liu CW, Ott T. RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections. Elife. 2023 Mar 1;12:e80741. doi: 10.7554/eLife.80741. PMID: 36856086; PMCID: PMC9991063." ## PMID- 36856086 OWN - NLM STAT- MEDLINE DCOM- 20230309 LR - 20230321 IS - 2050-084X (Electronic) IS - 2050-084X (Linking) VI - 12 DP - 2023 Mar 1 TI - RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections. LID - 10.7554/eLife.80741 [doi] LID - e80741 AB - Host-controlled intracellular accommodation of nitrogen-fixing bacteria is essential for the establishment of a functional Root Nodule Symbiosis (RNS). In many host plants, this occurs via transcellular tubular structures (infection threads - ITs) that extend across cell layers via polar tip-growth. Comparative phylogenomic studies have identified RPG (RHIZOBIUM-DIRECTED POLAR GROWTH) among the critical genetic determinants for bacterial infection. In Medicago truncatula, RPG is required for effective IT progression within root hairs but the cellular and molecular function of the encoded protein remains elusive. Here, we show that RPG resides in the protein complex formed by the core endosymbiotic components VAPYRIN (VPY) and LUMPY INFECTION (LIN) required for IT polar growth, co-localizes with both VPY and LIN in IT tip- and perinuclear-associated puncta of M. truncatula root hairs undergoing infection and is necessary for VPY recruitment into these structures. Fluorescence Lifetime Imaging Microscopy (FLIM) of phosphoinositide species during bacterial infection revealed that functional RPG is required to sustain strong membrane polarization at the advancing tip of the IT. In addition, loss of RPG functionality alters the cytoskeleton-mediated connectivity between the IT tip and the nucleus and affects the polar secretion of the cell wall modifying enzyme NODULE PECTATE LYASE (NPL). Our results integrate RPG into a core host machinery required to support symbiont accommodation, suggesting that its occurrence in plant host genomes is essential to co-opt a multimeric protein module committed to endosymbiosis to sustain IT-mediated bacterial infection. CI - (c) 2023, Lace et al. FAU - Lace, Beatrice AU - Lace B AUID- ORCID: 0000-0002-4732-573X AD - University of Freiburg, Faculty of Biology, Freiburg, Germany. FAU - Su, Chao AU - Su C AD - University of Freiburg, Faculty of Biology, Freiburg, Germany. FAU - Invernot Perez, Daniel AU - Invernot Perez D AD - University of Freiburg, Faculty of Biology, Freiburg, Germany. FAU - Rodriguez-Franco, Marta AU - Rodriguez-Franco M AUID- ORCID: 0000-0003-1183-2075 AD - University of Freiburg, Faculty of Biology, Freiburg, Germany. FAU - Vernie, Tatiana AU - Vernie T AD - LRSV, Universite de Toulouse, CNRS, UPS, INP Toulouse, Castanet-Tolosan, France. FAU - Batzenschlager, Morgane AU - Batzenschlager M AD - University of Freiburg, Faculty of Biology, Freiburg, Germany. FAU - Egli, Sabrina AU - Egli S AD - University of Freiburg, Faculty of Biology, Freiburg, Germany. FAU - Liu, Cheng-Wu AU - Liu CW AUID- ORCID: 0000-0002-6650-6245 AD - School of Life Sciences, Division of Life Sciences and Medicine, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science and Technology of China, Hefei, China. FAU - Ott, Thomas AU - Ott T AUID- ORCID: 0000-0002-4494-9811 AD - University of Freiburg, Faculty of Biology, Freiburg, Germany. AD - CIBSS - Centre of Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany. LA - eng GR - OPP1172165/GATES/Bill & Melinda Gates Foundation/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20230301 PL - England TA - Elife JT - eLife JID - 101579614 SB - IM UOF - doi: 10.1101/2022.06.03.494689 MH - *Rhizobium MH - Symbiosis MH - *Nitrogen-Fixing Bacteria MH - Cell Nucleus MH - Cell Wall PMC - PMC9991063 OTO - NOTNLM OT - infection thread OT - legume OT - nodulation OT - plant biology OT - polarity OT - rhizobia OT - symbiosis COIS- BL, CS, DI, MR, TV, MB, SE, CL, TO No competing interests declared EDAT- 2023/03/02 06:00 MHDA- 2023/03/10 06:00 PMCR- 2023/03/01 CRDT- 2023/03/01 05:54 PHST- 2022/06/01 00:00 [received] PHST- 2023/02/21 00:00 [accepted] PHST- 2023/03/02 06:00 [pubmed] PHST- 2023/03/10 06:00 [medline] PHST- 2023/03/01 05:54 [entrez] PHST- 2023/03/01 00:00 [pmc-release] AID - 80741 [pii] AID - 10.7554/eLife.80741 [doi] PST - epublish SO - Elife. 2023 Mar 1;12:e80741. doi: 10.7554/eLife.80741. ##### PUB RECORD ##### ## 10.1105/tpc.19.00609 32303662 Ribeiro, Lacchini et al., 2020 "Ribeiro B, Lacchini E, Bicalho KU, Mertens J, Arendt P, Vanden Bossche R, Calegario G, Gryffroy L, Ceulemans E, Buitink J, Goossens A, Pollier J. A Seed-Specific Regulator of Triterpene Saponin Biosynthesis in Medicago truncatula. Plant Cell. 2020 Jun;32(6):2020-2042. doi: 10.1105/tpc.19.00609. Epub 2020 Apr 17. PMID: 32303662; PMCID: PMC7268793." ## PMID- 32303662 OWN - NLM STAT- MEDLINE DCOM- 20210204 LR - 20210602 IS - 1532-298X (Electronic) IS - 1040-4651 (Print) IS - 1040-4651 (Linking) VI - 32 IP - 6 DP - 2020 Jun TI - A Seed-Specific Regulator of Triterpene Saponin Biosynthesis in Medicago truncatula. PG - 2020-2042 LID - 10.1105/tpc.19.00609 [doi] AB - Plants produce a vast array of defense compounds to protect themselves from pathogen attack or herbivore predation. Saponins are a specific class of defense compounds comprising bioactive glycosides with a steroidal or triterpenoid aglycone backbone. The model legume Medicago truncatula synthesizes two types of saponins, hemolytic saponins and nonhemolytic soyasaponins, which accumulate as specific blends in different plant organs. Here, we report the identification of the seed-specific transcription factor TRITERPENE SAPONIN ACTIVATION REGULATOR3 (TSAR3), which controls hemolytic saponin biosynthesis in developing M. truncatula seeds. Analysis of genes that are coexpressed with TSAR3 in transcriptome data sets from developing M. truncatula seeds led to the identification of CYP88A13, a cytochrome P450 that catalyzes the C-16alpha hydroxylation of medicagenic acid toward zanhic acid, the final oxidation step of the hemolytic saponin biosynthesis branch in M. truncatula In addition, two uridine diphosphate glycosyltransferases, UGT73F18 and UGT73F19, which glucosylate hemolytic sapogenins at the C-3 position, were identified. The genes encoding the identified biosynthetic enzymes are present in clusters of duplicated genes in the M. truncatula genome. This appears to be a common theme among saponin biosynthesis genes, especially glycosyltransferases, and may be the driving force of the metabolic evolution of saponins. CI - (c) 2020 American Society of Plant Biologists. All rights reserved. FAU - Ribeiro, Bianca AU - Ribeiro B AUID- ORCID: 0000-0002-1843-0258 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Lacchini, Elia AU - Lacchini E AUID- ORCID: 0000-0002-1598-8950 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Bicalho, Keylla U AU - Bicalho KU AUID- ORCID: 0000-0002-5165-9070 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. AD - Department of Organic Chemistry, Institute of Chemistry, Sao Paulo State University (UNESP), Araraquara, Sao Paulo 14800-900, Brazil. FAU - Mertens, Jan AU - Mertens J AUID- ORCID: 0000-0002-8095-0748 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Arendt, Philipp AU - Arendt P AUID- ORCID: 0000-0001-7429-0803 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Vanden Bossche, Robin AU - Vanden Bossche R AUID- ORCID: 0000-0001-6407-8139 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Calegario, Gabriela AU - Calegario G AUID- ORCID: 0000-0002-3772-8447 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Gryffroy, Lore AU - Gryffroy L AUID- ORCID: 0000-0001-9202-2992 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Ceulemans, Evi AU - Ceulemans E AUID- ORCID: 0000-0002-3083-5768 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Buitink, Julia AU - Buitink J AUID- ORCID: 0000-0002-1457-764X AD - Institut de Recherche en Horticulture et Semences-Unites Mixtes de Recherche, Universite d'Angers, INRAE, Institut Agro, SFR 4207 QuaSaV, 49071 Beaucouze, France. FAU - Goossens, Alain AU - Goossens A AUID- ORCID: 0000-0002-1599-551X AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium alain.goossens@psb-vib.ugent.be jacob.pollier@psb-vib.ugent.be. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. FAU - Pollier, Jacob AU - Pollier J AUID- ORCID: 0000-0002-1134-9238 AD - Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium alain.goossens@psb-vib.ugent.be jacob.pollier@psb-vib.ugent.be. AD - VIB Center for Plant Systems Biology, 9052 Ghent, Belgium. AD - VIB Metabolomics Core, 9052 Ghent, Belgium. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200417 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 RN - 0 (Plant Proteins) RN - 0 (Triterpenes) SB - IM MH - Gene Expression Regulation, Plant MH - Medicago truncatula/genetics/*metabolism MH - Plant Proteins/genetics/*metabolism MH - Seeds/*metabolism MH - Triterpenes/*metabolism PMC - PMC7268793 EDAT- 2020/04/19 06:00 MHDA- 2021/02/05 06:00 PMCR- 2021/06/01 CRDT- 2020/04/19 06:00 PHST- 2019/08/07 00:00 [received] PHST- 2020/03/27 00:00 [revised] PHST- 2020/04/10 00:00 [accepted] PHST- 2020/04/19 06:00 [pubmed] PHST- 2021/02/05 06:00 [medline] PHST- 2020/04/19 06:00 [entrez] PHST- 2021/06/01 00:00 [pmc-release] AID - tpc.19.00609 [pii] AID - 201900609R2 [pii] AID - 10.1105/tpc.19.00609 [doi] PST - ppublish SO - Plant Cell. 2020 Jun;32(6):2020-2042. doi: 10.1105/tpc.19.00609. Epub 2020 Apr 17. ##### PUB RECORD ##### ## 10.1111/j.1365-313X.2009.04072.x 19912567 Pumplin, Mondo et al., 2009 "Pumplin N, Mondo SJ, Topp S, Starker CG, Gantt JS, Harrison MJ. Medicago truncatula Vapyrin is a novel protein required for arbuscular mycorrhizal symbiosis. Plant J. 2010 Feb 1;61(3):482-94. doi: 10.1111/j.1365-313X.2009.04072.x. Epub 2009 Nov 14. PMID: 19912567." ## PMID- 19912567 OWN - NLM STAT- MEDLINE DCOM- 20100830 LR - 20240109 IS - 1365-313X (Electronic) IS - 0960-7412 (Linking) VI - 61 IP - 3 DP - 2010 Feb 1 TI - Medicago truncatula Vapyrin is a novel protein required for arbuscular mycorrhizal symbiosis. PG - 482-94 LID - 10.1111/j.1365-313X.2009.04072.x [doi] AB - Arbuscular mycorrhizal (AM) symbiosis is a widespread mutualism formed between vascular plants and fungi of the Glomeromycota. In this endosymbiosis, fungal hyphae enter the roots, growing through epidermal cells to the cortex where they establish differentiated hyphae called arbuscules in the cortical cells. Reprogramming of the plant epidermal and cortical cells occurs to enable intracellular growth of the fungal symbiont; however, the plant genes underlying this process are largely unknown. Here, through the use of RNAi, we demonstrate that the expression of a Medicago truncatula gene named Vapyrin is essential for arbuscule formation, and also for efficient epidermal penetration by AM fungi. Vapyrin is induced transiently in the epidermis coincident with hyphal penetration, and then in the cortex during arbuscule formation. The Vapyrin protein is cytoplasmic, and in cells containing AM fungal hyphae, the protein accumulates in small puncta that move through the cytoplasm. Vapyrin is a novel protein composed of two domains that mediate protein-protein interactions: an N-terminal VAMP-associated protein (VAP)/major sperm protein (MSP) domain and a C-terminal ankyrin-repeat domain. Putative Vapyrin orthologs exist widely in the plant kingdom, but not in Arabidopsis, or in non-plant species. The data suggest a role for Vapyrin in cellular remodeling to support the intracellular development of fungal hyphae during AM symbiosis. FAU - Pumplin, Nathan AU - Pumplin N AD - Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA. FAU - Mondo, Stephen J AU - Mondo SJ FAU - Topp, Stephanie AU - Topp S FAU - Starker, Colby G AU - Starker CG FAU - Gantt, J Stephen AU - Gantt JS FAU - Harrison, Maria J AU - Harrison MJ LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20091114 PL - England TA - Plant J JT - The Plant journal : for cell and molecular biology JID - 9207397 RN - 0 (Plant Proteins) RN - 0 (Vesicular Transport Proteins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Gene Expression Regulation, Plant MH - Humans MH - Medicago truncatula/chemistry/genetics/*metabolism MH - Molecular Sequence Data MH - Mycorrhizae/*metabolism MH - Phylogeny MH - Plant Proteins/chemistry/genetics/*metabolism MH - RNA Interference MH - *Symbiosis MH - Vesicular Transport Proteins/chemistry/genetics/*metabolism EDAT- 2009/11/17 06:00 MHDA- 2010/08/31 06:00 CRDT- 2009/11/17 06:00 PHST- 2009/11/17 06:00 [entrez] PHST- 2009/11/17 06:00 [pubmed] PHST- 2010/08/31 06:00 [medline] AID - TPJ4072 [pii] AID - 10.1111/j.1365-313X.2009.04072.x [doi] PST - ppublish SO - Plant J. 2010 Feb 1;61(3):482-94. doi: 10.1111/j.1365-313X.2009.04072.x. Epub 2009 Nov 14. ##### PUB RECORD ##### ## 10.1104/pp.18.01572 30710053 Liu, Breakspear et al., 2019 "Liu CW, Breakspear A, Guan D, Cerri MR, Jackson K, Jiang S, Robson F, Radhakrishnan GV, Roy S, Bone C, Stacey N, Rogers C, Trick M, Niebel A, Oldroyd GED, de Carvalho-Niebel F, Murray JD. NIN Acts as a Network Hub Controlling a Growth Module Required for Rhizobial Infection. Plant Physiol. 2019 Apr;179(4):1704-1722. doi: 10.1104/pp.18.01572. Epub 2019 Feb 1. PMID: 30710053; PMCID: PMC6446755." ## PMID- 30710053 OWN - NLM STAT- MEDLINE DCOM- 20190618 LR - 20200225 IS - 1532-2548 (Electronic) IS - 0032-0889 (Print) IS - 0032-0889 (Linking) VI - 179 IP - 4 DP - 2019 Apr TI - NIN Acts as a Network Hub Controlling a Growth Module Required for Rhizobial Infection. PG - 1704-1722 LID - 10.1104/pp.18.01572 [doi] AB - The symbiotic infection of root cells by nitrogen-fixing rhizobia during nodulation requires the transcription factor Nodule Inception (NIN). Our root hair transcriptomic study extends NIN's regulon to include Rhizobium Polar Growth and genes involved in cell wall modification, gibberellin biosynthesis, and a comprehensive group of nutrient (N, P, and S) uptake and assimilation genes, suggesting that NIN's recruitment to nodulation was based on its role as a growth module, a role shared with other NIN-Like Proteins. The expression of jasmonic acid genes in nin suggests the involvement of NIN in the resolution of growth versus defense outcomes. We find that the regulation of the growth module component Nodulation Pectate Lyase by NIN, and its function in rhizobial infection, are conserved in hologalegina legumes, highlighting its recruitment as a major event in the evolution of nodulation. We find that Nodulation Pectate Lyase is secreted to the infection chamber and the lumen of the infection thread. Gene network analysis using the transcription factor mutants for ERF Required for Nodulation1 and Nuclear Factor-Y Subunit A1 confirms hierarchical control of NIN over Nuclear Factor-Y Subunit A1 and shows that ERF Required for Nodulation1 acts independently to control infection. We conclude that while NIN shares functions with other NIN-Like Proteins, the conscription of key infection genes to NIN's control has made it a central regulatory hub for rhizobial infection. CI - (c) 2019 American Society of Plant Biologists. All Rights Reserved. FAU - Liu, Cheng-Wu AU - Liu CW AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Breakspear, Andrew AU - Breakspear A AUID- ORCID: 0000-0002-1863-3012 AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Guan, Dian AU - Guan D AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Cerri, Marion R AU - Cerri MR AD - Laboratory of Plant Microbe Interactions, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Universite de Toulouse, 31326 Castanet-Tolosan, France. FAU - Jackson, Kirsty AU - Jackson K AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Jiang, Suyu AU - Jiang S AD - National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Centre of Excellence for Plant and Microbial Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China. FAU - Robson, Fran AU - Robson F AUID- ORCID: 0000-0003-2734-5124 AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Radhakrishnan, Guru V AU - Radhakrishnan GV AUID- ORCID: 0000-0003-0381-8804 AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Roy, Sonali AU - Roy S AUID- ORCID: 0000-0002-8114-8300 AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Bone, Caitlin AU - Bone C AUID- ORCID: 0000-0002-5881-3202 AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Stacey, Nicola AU - Stacey N AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Rogers, Christian AU - Rogers C AUID- ORCID: 0000-0003-2887-3260 AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. AD - Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, United Kingdom. FAU - Trick, Martin AU - Trick M AUID- ORCID: 0000-0001-8786-5012 AD - Computational and Systems Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Niebel, Andreas AU - Niebel A AUID- ORCID: 0000-0002-3402-8381 AD - Laboratory of Plant Microbe Interactions, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Universite de Toulouse, 31326 Castanet-Tolosan, France. FAU - Oldroyd, Giles E D AU - Oldroyd GED AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. AD - Computational and Systems Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - de Carvalho-Niebel, Fernanda AU - de Carvalho-Niebel F AUID- ORCID: 0000-0002-5596-9420 AD - Laboratory of Plant Microbe Interactions, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Universite de Toulouse, 31326 Castanet-Tolosan, France. FAU - Murray, Jeremy D AU - Murray JD AUID- ORCID: 0000-0003-3000-9199 AD - Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom jeremy.murray@jic.ac.uk. AD - National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Centre of Excellence for Plant and Microbial Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China. LA - eng GR - BB/L010305/1/Biotechnology and Biological Sciences Research Council/United Kingdom GR - BB/010305/1/Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190201 PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 RN - 0 (Cyclopentanes) RN - 0 (Gibberellins) RN - 0 (NIN protein, Lotus japonicus) RN - 0 (Oxylipins) RN - 0 (Plant Proteins) RN - 6RI5N05OWW (jasmonic acid) SB - IM MH - Biosynthetic Pathways/genetics MH - Cyclopentanes/metabolism MH - Gene Expression Regulation, Plant MH - Gene Regulatory Networks MH - Gibberellins/biosynthesis MH - Medicago truncatula/*genetics/microbiology MH - Oxylipins/metabolism MH - Plant Proteins/genetics/metabolism/*physiology MH - Rhizobium/genetics/*physiology PMC - PMC6446755 EDAT- 2019/02/03 06:00 MHDA- 2019/06/19 06:00 PMCR- 2019/02/01 CRDT- 2019/02/03 06:00 PHST- 2019/01/03 00:00 [received] PHST- 2019/01/20 00:00 [accepted] PHST- 2019/02/03 06:00 [pubmed] PHST- 2019/06/19 06:00 [medline] PHST- 2019/02/03 06:00 [entrez] PHST- 2019/02/01 00:00 [pmc-release] AID - pp.18.01572 [pii] AID - 201801572D [pii] AID - 10.1104/pp.18.01572 [doi] PST - ppublish SO - Plant Physiol. 2019 Apr;179(4):1704-1722. doi: 10.1104/pp.18.01572. Epub 2019 Feb 1. ##### PUB RECORD ##### ## 10.1105/tpc.15.00461 26672071 Vernie, Kim et al., 2015 "Vernié T, Kim J, Frances L, Ding Y, Sun J, Guan D, Niebel A, Gifford ML, de Carvalho-Niebel F, Oldroyd GE. The NIN Transcription Factor Coordinates Diverse Nodulation Programs in Different Tissues of the Medicago truncatula Root. Plant Cell. 2015 Dec;27(12):3410-24. doi: 10.1105/tpc.15.00461. Epub 2015 Dec 15. PMID: 26672071; PMCID: PMC4707452." ## PMID- 26672071 OWN - NLM STAT- MEDLINE DCOM- 20170130 LR - 20231213 IS - 1532-298X (Electronic) IS - 1040-4651 (Print) IS - 1040-4651 (Linking) VI - 27 IP - 12 DP - 2015 Dec TI - The NIN Transcription Factor Coordinates Diverse Nodulation Programs in Different Tissues of the Medicago truncatula Root. PG - 3410-24 LID - 10.1105/tpc.15.00461 [doi] AB - Biological nitrogen fixation in legumes occurs in nodules that are initiated in the root cortex following Nod factor recognition at the root surface, and this requires coordination of diverse developmental programs in these different tissues. We show that while early Nod factor signaling associated with calcium oscillations is limited to the root surface, the resultant activation of Nodule Inception (NIN) in the root epidermis is sufficient to promote cytokinin signaling and nodule organogenesis in the inner root cortex. NIN or a product of its action must be associated with the transmission of a signal between the root surface and the cortical cells where nodule organogenesis is initiated. NIN appears to have distinct functions in the root epidermis and the root cortex. In the epidermis, NIN restricts the extent of Early Nodulin 11 (ENOD11) expression and does so through competitive inhibition of ERF Required for Nodulation (ERN1). In contrast, NIN is sufficient to promote the expression of the cytokinin receptor Cytokinin Response 1 (CRE1), which is restricted to the root cortex. Our work in Medicago truncatula highlights the complexity of NIN action and places NIN as a central player in the coordination of the symbiotic developmental programs occurring in differing tissues of the root that combined are necessary for a nitrogen-fixing symbiosis. CI - (c) 2015 American Society of Plant Biologists. All rights reserved. FAU - Vernie, Tatiana AU - Vernie T AUID- ORCID: 0000-0003-1387-6370 AD - Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Kim, Jiyoung AU - Kim J AD - Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Frances, Lisa AU - Frances L AD - Laboratoire des Interactions Plantes Microorganismes, CNRS-INRA 2594/441, F-31320 Castanet Tolosan, France. FAU - Ding, Yiliang AU - Ding Y AD - Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Sun, Jongho AU - Sun J AD - Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Guan, Dian AU - Guan D AD - Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom. FAU - Niebel, Andreas AU - Niebel A AUID- ORCID: 0000-0002-3402-8381 AD - Laboratoire des Interactions Plantes Microorganismes, CNRS-INRA 2594/441, F-31320 Castanet Tolosan, France. FAU - Gifford, Miriam L AU - Gifford ML AUID- ORCID: 0000-0002-4005-2513 AD - School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. FAU - de Carvalho-Niebel, Fernanda AU - de Carvalho-Niebel F AD - Laboratoire des Interactions Plantes Microorganismes, CNRS-INRA 2594/441, F-31320 Castanet Tolosan, France. FAU - Oldroyd, Giles E D AU - Oldroyd GE AD - Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom giles.oldroyd@jic.ac.uk. LA - eng GR - BB/H019502/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom GR - BB/J001872/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151215 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 RN - 0 (Cytokinins) RN - 0 (ENOD11 protein, Medicago truncatula) RN - 0 (NIN protein, Lotus japonicus) RN - 0 (Plant Growth Regulators) RN - 0 (Plant Proteins) RN - 0 (Transcription Factors) RN - SY7Q814VUP (Calcium) SB - IM MH - Calcium/metabolism MH - Cytokinins/metabolism MH - Gene Expression Regulation, Plant MH - Genes, Reporter MH - Medicago truncatula/cytology/*genetics/physiology MH - Nitrogen Fixation MH - Plant Growth Regulators/metabolism MH - Plant Proteins/genetics/*metabolism MH - Plant Root Nodulation MH - Plant Roots/cytology/genetics/metabolism/physiology MH - Plants, Genetically Modified MH - Root Nodules, Plant/cytology/genetics/physiology MH - *Signal Transduction MH - Sinorhizobium meliloti/*physiology MH - *Symbiosis MH - Nicotiana/cytology/genetics/physiology MH - Transcription Factors/genetics/*metabolism PMC - PMC4707452 EDAT- 2015/12/17 06:00 MHDA- 2017/01/31 06:00 PMCR- 2015/12/15 CRDT- 2015/12/17 06:00 PHST- 2015/05/21 00:00 [received] PHST- 2015/11/20 00:00 [accepted] PHST- 2015/12/17 06:00 [entrez] PHST- 2015/12/17 06:00 [pubmed] PHST- 2017/01/31 06:00 [medline] PHST- 2015/12/15 00:00 [pmc-release] AID - tpc.15.00461 [pii] AID - TPC201500461RAR2 [pii] AID - 10.1105/tpc.15.00461 [doi] PST - ppublish SO - Plant Cell. 2015 Dec;27(12):3410-24. doi: 10.1105/tpc.15.00461. Epub 2015 Dec 15. ##### PUB RECORD ##### ## 10.1093/jxb/erab033 33506247 Zhou, Yang et al., 2023 "Zhou S, Yang T, Mao Y, Liu Y, Guo S, Wang R, Fangyue G, He L, Zhao B, Bai Q, Li Y, Zhang X, Wang D, Wang C, Wu Q, Yang Y, Liu Y, Tadege M, Chen J. The F-box protein MIO1/SLB1 regulates organ size and leaf movement in Medicago truncatula. J Exp Bot. 2021 Apr 2;72(8):2995-3011. doi: 10.1093/jxb/erab033. PMID: 33506247; PMCID: PMC8023213." ## PMID- 33506247 OWN - NLM STAT- MEDLINE DCOM- 20210521 LR - 20220531 IS - 1460-2431 (Electronic) IS - 0022-0957 (Print) IS - 0022-0957 (Linking) VI - 72 IP - 8 DP - 2021 Apr 2 TI - The F-box protein MIO1/SLB1 regulates organ size and leaf movement in Medicago truncatula. PG - 2995-3011 LID - 10.1093/jxb/erab033 [doi] AB - The size of leaf and seed organs, determined by the interplay of cell proliferation and expansion, is closely related to the final yield and quality of forage and crops. Yet the cellular and molecular mechanisms underlying organ size modulation remain poorly understood, especially in legumes. Here, MINI ORGAN1 (MIO1), which encodes an F-box protein SMALL LEAF AND BUSHY1 (SLB1) recently reported to control lateral branching in Medicago truncatula, was identified as a key regulator of organ size. We show that loss-of-function of MIO1/SLB1 severely reduced organ size. Conversely, plants overexpressing MIO1/SLB1 had enlarged organs. Cellular analysis revealed that MIO1/SLB1 controlled organ size mainly by modulating primary cell proliferation during the early stages of leaf development. Biochemical analysis revealed that MIO1/SLB1 could form part of SKP1/Cullin/F-box (SCF) E3 ubiquitin ligase complex, to target BIG SEEDS1 (BS1), a repressor of primary cell division, for degradation. Interestingly, we found that MIO1/SLB1 also played a key role in pulvinus development and leaf movement by modulating cell proliferation of the pulvinus as leaves developed. Our study not only demonstrates a conserved role of MIO1/SLB1 in the control of organ size in legumes, but also sheds light on the novel function of MIO1/SLB1 in leaf movement. CI - (c) The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. FAU - Zhou, Shaoli AU - Zhou S AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Yang, Tianquan AU - Yang T AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China. FAU - Mao, Yawen AU - Mao Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Liu, Ye AU - Liu Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - School of Life Sciences, University of Science and Technology of China, Hefei, China. FAU - Guo, Shiqi AU - Guo S AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Wang, Ruoruo AU - Wang R AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Fangyue, Genwang AU - Fangyue G AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - He, Liangliang AU - He L AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Zhao, Baolin AU - Zhao B AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. FAU - Bai, Quanzi AU - Bai Q AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Li, Youhan AU - Li Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. FAU - Zhang, Xiaojia AU - Zhang X AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Wang, Dongfa AU - Wang D AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - School of Life Sciences, University of Science and Technology of China, Hefei, China. FAU - Wang, Chaoqun AU - Wang C AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Wu, Qing AU - Wu Q AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Yang, Yuanfan AU - Yang Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - School of Ecology and Environmental Sciences, Yunnan University, Kunming, China. FAU - Liu, Yu AU - Liu Y AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. FAU - Tadege, Million AU - Tadege M AD - Department of Plant and Soil Sciences, Institute for Agricultural Biosciences, Oklahoma State University, 3210 Sam Noble Parkway, Ardmore, OK, USA. FAU - Chen, Jianghua AU - Chen J AD - CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence for Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China. AD - University of Chinese Academy of Sciences, Beijing, China. AD - School of Life Sciences, University of Science and Technology of China, Hefei, China. AD - School of Ecology and Environmental Sciences, Yunnan University, Kunming, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Exp Bot JT - Journal of experimental botany JID - 9882906 RN - 0 (Cullin Proteins) RN - 0 (F-Box Proteins) RN - 0 (Plant Proteins) RN - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases) RN - EC 2.3.2.27 (Ubiquitin-Protein Ligases) SB - IM MH - Cullin Proteins/metabolism MH - *F-Box Proteins MH - *Medicago truncatula/genetics/metabolism MH - Organ Size MH - Plant Leaves MH - *Plant Proteins MH - SKP Cullin F-Box Protein Ligases/metabolism MH - Ubiquitin-Protein Ligases/metabolism PMC - PMC8023213 OTO - NOTNLM OT - BIG SEEDS1 (BS1) OT - F-box protein OT - MINI ORGAN1 (MIO1)/SMALL LEAF AND BUSHY1 (SLB1) OT - SCF E3 ligase OT - organ size OT - proteasome-mediated degradation OT - pulvinus EDAT- 2021/01/29 06:00 MHDA- 2021/05/22 06:00 PMCR- 2021/01/28 CRDT- 2021/01/28 05:45 PHST- 2020/04/04 00:00 [received] PHST- 2021/01/26 00:00 [accepted] PHST- 2021/01/29 06:00 [pubmed] PHST- 2021/05/22 06:00 [medline] PHST- 2021/01/28 05:45 [entrez] PHST- 2021/01/28 00:00 [pmc-release] AID - 6121689 [pii] AID - erab033 [pii] AID - 10.1093/jxb/erab033 [doi] PST - ppublish SO - J Exp Bot. 2021 Apr 2;72(8):2995-3011. doi: 10.1093/jxb/erab033. ##### PUB RECORD ##### ## 10.1105/tpc.15.00476 26410301 Jun, Liu et al., 2015 "Jun JH, Liu C, Xiao X, Dixon RA. The Transcriptional Repressor MYB2 Regulates Both Spatial and Temporal Patterns of Proanthocyandin and Anthocyanin Pigmentation in Medicago truncatula. Plant Cell. 2015 Oct;27(10):2860-79. doi: 10.1105/tpc.15.00476. Epub 2015 Sep 26. PMID: 26410301; PMCID: PMC4682322." ## PMID- 26410301 OWN - NLM STAT- MEDLINE DCOM- 20170905 LR - 20240324 IS - 1532-298X (Electronic) IS - 1040-4651 (Print) IS - 1040-4651 (Linking) VI - 27 IP - 10 DP - 2015 Oct TI - The Transcriptional Repressor MYB2 Regulates Both Spatial and Temporal Patterns of Proanthocyandin and Anthocyanin Pigmentation in Medicago truncatula. PG - 2860-79 LID - 10.1105/tpc.15.00476 [doi] AB - Accumulation of anthocyanins and proanthocyanidins (PAs) is limited to specific cell types and developmental stages, but little is known about how antagonistically acting transcriptional regulators work together to determine temporal and spatial patterning of pigmentation at the cellular level, especially for PAs. Here, we characterize MYB2, a transcriptional repressor regulating both anthocyanin and PA biosynthesis in the model legume Medicago truncatula. MYB2 was strongly upregulated by MYB5, a major regulator of PA biosynthesis in M. truncatula and a component of MYB-basic helix loop helix-WD40 (MBW) activator complexes. Overexpression of MYB2 abolished anthocyanin and PA accumulation in M. truncatula hairy roots and Arabidopsis thaliana seeds, respectively. Anthocyanin deposition was expanded in myb2 mutant seedlings and flowers accompanied by increased anthocyanin content. PA mainly accumulated in the epidermal layer derived from the outer integument in the M. truncatula seed coat, starting from the hilum area. The area of PA accumulation and ANTHOCYANIDIN REDUCTASE expression was expanded into the seed body at the early stage of seed development in the myb2 mutant. Genetic, biochemical, and cell biological evidence suggests that MYB2 functions as part of a multidimensional regulatory network to define the temporal and spatial pattern of anthocyanin and PA accumulation linked to developmental processes. CI - (c) 2015 American Society of Plant Biologists. All rights reserved. FAU - Jun, Ji Hyung AU - Jun JH AUID- ORCID: 0000-0002-2563-4144 AD - Department of Biological Sciences, University of North Texas, Denton, Texas 76203-5017. FAU - Liu, Chenggang AU - Liu C AUID- ORCID: 0000-0002-0576-8567 AD - Department of Biological Sciences, University of North Texas, Denton, Texas 76203-5017. FAU - Xiao, Xirong AU - Xiao X AUID- ORCID: 0000-0002-1785-6417 AD - Department of Biological Sciences, University of North Texas, Denton, Texas 76203-5017. FAU - Dixon, Richard A AU - Dixon RA AUID- ORCID: 0000-0001-8393-9408 AD - Department of Biological Sciences, University of North Texas, Denton, Texas 76203-5017 richard.dixon@unt.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150926 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 RN - 0 (Anthocyanins) RN - 0 (Plant Proteins) RN - 0 (Proanthocyanidins) RN - 0 (Transcription Factors) RN - EC 1.- (Oxidoreductases) SB - IM MH - Anthocyanins/*metabolism MH - Arabidopsis/cytology/genetics/metabolism MH - Flowers/cytology/genetics/metabolism MH - Gene Expression MH - *Gene Expression Regulation, Plant MH - Medicago truncatula/cytology/*genetics/metabolism MH - Mutation MH - Oxidoreductases/genetics/metabolism MH - Phylogeny MH - Pigmentation MH - Plant Proteins/genetics/metabolism MH - Plant Roots/cytology/genetics/metabolism MH - Plants, Genetically Modified MH - Proanthocyanidins/*metabolism MH - Seedlings/cytology/genetics/metabolism MH - Seeds/cytology/genetics/metabolism MH - Transcription Factors/genetics/*metabolism PMC - PMC4682322 EDAT- 2015/09/28 06:00 MHDA- 2017/09/07 06:00 PMCR- 2015/09/28 CRDT- 2015/09/28 06:00 PHST- 2015/05/29 00:00 [received] PHST- 2015/09/10 00:00 [accepted] PHST- 2015/09/28 06:00 [entrez] PHST- 2015/09/28 06:00 [pubmed] PHST- 2017/09/07 06:00 [medline] PHST- 2015/09/28 00:00 [pmc-release] AID - tpc.15.00476 [pii] AID - TPC201500476RAR2 [pii] AID - 10.1105/tpc.15.00476 [doi] PST - ppublish SO - Plant Cell. 2015 Oct;27(10):2860-79. doi: 10.1105/tpc.15.00476. Epub 2015 Sep 26. ##### PUB RECORD ##### ## 10.1186/s13007-015-0053-y 25774204 Oellrich, Walls et al., 2015 "Oellrich A, Walls RL, Cannon EK, Cannon SB, Cooper L, Gardiner J, Gkoutos GV, Harper L, He M, Hoehndorf R, Jaiswal P, Kalberer SR, Lloyd JP, Meinke D, Menda N, Moore L, Nelson RT, Pujar A, Lawrence CJ, Huala E. An ontology approach to comparative phenomics in plants. Plant Methods. 2015 Feb 25;11:10. doi: 10.1186/s13007-015-0053-y. PMID: 25774204; PMCID: PMC4359497." ## PMID- 25774204 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20150316 LR - 20220310 IS - 1746-4811 (Print) IS - 1746-4811 (Electronic) IS - 1746-4811 (Linking) VI - 11 DP - 2015 TI - An ontology approach to comparative phenomics in plants. PG - 10 LID - 10.1186/s13007-015-0053-y [doi] LID - 10 AB - BACKGROUND: Plant phenotype datasets include many different types of data, formats, and terms from specialized vocabularies. Because these datasets were designed for different audiences, they frequently contain language and details tailored to investigators with different research objectives and backgrounds. Although phenotype comparisons across datasets have long been possible on a small scale, comprehensive queries and analyses that span a broad set of reference species, research disciplines, and knowledge domains continue to be severely limited by the absence of a common semantic framework. RESULTS: We developed a workflow to curate and standardize existing phenotype datasets for six plant species, encompassing both model species and crop plants with established genetic resources. Our effort focused on mutant phenotypes associated with genes of known sequence in Arabidopsis thaliana (L.) Heynh. (Arabidopsis), Zea mays L. subsp. mays (maize), Medicago truncatula Gaertn. (barrel medic or Medicago), Oryza sativa L. (rice), Glycine max (L.) Merr. (soybean), and Solanum lycopersicum L. (tomato). We applied the same ontologies, annotation standards, formats, and best practices across all six species, thereby ensuring that the shared dataset could be used for cross-species querying and semantic similarity analyses. Curated phenotypes were first converted into a common format using taxonomically broad ontologies such as the Plant Ontology, Gene Ontology, and Phenotype and Trait Ontology. We then compared ontology-based phenotypic descriptions with an existing classification system for plant phenotypes and evaluated our semantic similarity dataset for its ability to enhance predictions of gene families, protein functions, and shared metabolic pathways that underlie informative plant phenotypes. CONCLUSIONS: The use of ontologies, annotation standards, shared formats, and best practices for cross-taxon phenotype data analyses represents a novel approach to plant phenomics that enhances the utility of model genetic organisms and can be readily applied to species with fewer genetic resources and less well-characterized genomes. In addition, these tools should enhance future efforts to explore the relationships among phenotypic similarity, gene function, and sequence similarity in plants, and to make genotype-to-phenotype predictions relevant to plant biology, crop improvement, and potentially even human health. FAU - Oellrich, Anika AU - Oellrich A AD - Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA UK. FAU - Walls, Ramona L AU - Walls RL AD - iPlant Collaborative, University of Arizona, 1657 E. Helen St., Tucson, Arizona 85721 USA. FAU - Cannon, Ethalinda Ks AU - Cannon EK AD - Department of Electrical and Computer Engineering Iowa State University, 1018 Crop Informatics Lab, Ames, Iowa 50011 USA. FAU - Cannon, Steven B AU - Cannon SB AD - USDA-ARS Corn Insects and Crop Genetics Research Unit, Iowa State University, Crop Genome Informatics Lab, Iowa State University, Ames, IA 50011 USA. AD - Department of Agronomy, Agronomy Hall, Iowa State University, Ames, IA 50010 USA. FAU - Cooper, Laurel AU - Cooper L AD - Department of Botany and Plant Pathology, 2082 Cordley Hall, Oregon State University, Corvallis, OR 97331 USA. FAU - Gardiner, Jack AU - Gardiner J AD - Department of Genetics, Development and Cell Biology, Roy J Carver Co-Laboratory, Iowa State University, Ames, IA 50010 USA. FAU - Gkoutos, Georgios V AU - Gkoutos GV AD - Department of Computer Science, Aberystwyth University, Llandinam Building, Aberystwyth, SY23 3DB UK. FAU - Harper, Lisa AU - Harper L AD - USDA-ARS Corn Insects and Crop Genetics Research Unit, Iowa State University, Crop Genome Informatics Lab, Iowa State University, Ames, IA 50011 USA. FAU - He, Mingze AU - He M AD - Department of Genetics, Development and Cell Biology, Roy J Carver Co-Laboratory, Iowa State University, Ames, IA 50010 USA. FAU - Hoehndorf, Robert AU - Hoehndorf R AD - Computer, Electrical and Mathematical Sciences & Engineering Division and Computational Bioscience Research Center, King Abdullah University of Science and Technology, 4700 King Abdullah University of Science and Technology, P.O. Box 2882, Thuwal, 23955-6900 Kingdom of Saudi Arabia. FAU - Jaiswal, Pankaj AU - Jaiswal P AD - Department of Botany and Plant Pathology, 2082 Cordley Hall, Oregon State University, Corvallis, OR 97331 USA. FAU - Kalberer, Scott R AU - Kalberer SR AD - USDA-ARS Corn Insects and Crop Genetics Research Unit, Iowa State University, Crop Genome Informatics Lab, Iowa State University, Ames, IA 50011 USA. FAU - Lloyd, John P AU - Lloyd JP AD - Department of Plant Biology, Michigan State University, 220 Trowbridge Rd, East Lansing, MI 48824 USA. FAU - Meinke, David AU - Meinke D AD - Department of Botany, Oklahoma State University, 301 Physical Sciences, Stillwater, OK 74078 USA. FAU - Menda, Naama AU - Menda N AD - Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, NY 14853 USA. FAU - Moore, Laura AU - Moore L AD - Department of Botany and Plant Pathology, 2082 Cordley Hall, Oregon State University, Corvallis, OR 97331 USA. FAU - Nelson, Rex T AU - Nelson RT AD - USDA-ARS Corn Insects and Crop Genetics Research Unit, Iowa State University, Crop Genome Informatics Lab, Iowa State University, Ames, IA 50011 USA. FAU - Pujar, Anuradha AU - Pujar A AD - Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, NY 14853 USA. FAU - Lawrence, Carolyn J AU - Lawrence CJ AD - Department of Agronomy, Agronomy Hall, Iowa State University, Ames, IA 50010 USA. AD - Department of Genetics, Development and Cell Biology, Roy J Carver Co-Laboratory, Iowa State University, Ames, IA 50010 USA. FAU - Huala, Eva AU - Huala E AD - Phoenix Bioinformatics, 643 Bair Island Rd Suite 403, Redwood City, CA 94063 USA. LA - eng PT - Journal Article DEP - 20150225 PL - England TA - Plant Methods JT - Plant methods JID - 101245798 PMC - PMC4359497 EDAT- 2015/03/17 06:00 MHDA- 2015/03/17 06:01 PMCR- 2015/02/25 CRDT- 2015/03/17 06:00 PHST- 2014/12/08 00:00 [received] PHST- 2015/02/05 00:00 [accepted] PHST- 2015/03/17 06:00 [entrez] PHST- 2015/03/17 06:00 [pubmed] PHST- 2015/03/17 06:01 [medline] PHST- 2015/02/25 00:00 [pmc-release] AID - 53 [pii] AID - 10.1186/s13007-015-0053-y [doi] PST - epublish SO - Plant Methods. 2015 Feb 25;11:10. doi: 10.1186/s13007-015-0053-y. eCollection 2015. ##### PUB RECORD ##### ## 10.1186/s12870-020-02619-6 32867687 Jiao, Wang et al., 2020 "Jiao Z, Wang L, Du H, Wang Y, Wang W, Liu J, Huang J, Huang W, Ge L. Genome-wide study of C2H2 zinc finger gene family in Medicago truncatula. BMC Plant Biol. 2020 Aug 31;20(1):401. doi: 10.1186/s12870-020-02619-6. PMID: 32867687; PMCID: PMC7460785." ## PMID- 32867687 OWN - NLM STAT- MEDLINE DCOM- 20210218 LR - 20210218 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 20 IP - 1 DP - 2020 Aug 31 TI - Genome-wide study of C2H2 zinc finger gene family in Medicago truncatula. PG - 401 LID - 10.1186/s12870-020-02619-6 [doi] LID - 401 AB - BACKGROUND: C2H2 zinc finger proteins (C2H2 ZFPs) play vital roles in shaping many aspects of plant growth and adaptation to the environment. Plant genomes harbor hundreds of C2H2 ZFPs, which compose one of the most important and largest transcription factor families in higher plants. Although the C2H2 ZFP gene family has been reported in several plant species, it has not been described in the model leguminous species Medicago truncatula. RESULTS: In this study, we identified 218 C2H2 type ZFPs with 337 individual C2H2 motifs in M. truncatula. We showed that the high rate of local gene duplication has significantly contributed to the expansion of the C2H2 gene family in M. truncatula. The identified ZFPs exhibit high variation in motif arrangement and expression pattern, suggesting that the short C2H2 zinc finger motif has been adopted as a scaffold by numerous transcription factors with different functions to recognize cis-elements. By analyzing the public expression datasets and quantitative RT-PCR (qRT-PCR), we identified several C2H2 ZFPs that are specifically expressed in certain tissues, such as the nodule, seed, and flower. CONCLUSION: Our genome-wide work revealed an expanded C2H2 ZFP gene family in an important legume M. truncatula, and provides new insights into the diversification and expansion of C2H2 ZFPs in higher plants. FAU - Jiao, Zhicheng AU - Jiao Z AD - Department of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, Guangdong, China. AD - Guangdong Engineering Research Center for Grassland Science, Tianhe, 483 Wushan Road, Guangzhou, 510642, Guangdong, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, 510642, Guangdong, China. FAU - Wang, Liping AU - Wang L AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, China. AD - College of Life Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong, China. FAU - Du, Huan AU - Du H AD - Department of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, Guangdong, China. AD - Guangdong Engineering Research Center for Grassland Science, Tianhe, 483 Wushan Road, Guangzhou, 510642, Guangdong, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, 510642, Guangdong, China. FAU - Wang, Ying AU - Wang Y AD - Department of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, Guangdong, China. AD - Guangdong Engineering Research Center for Grassland Science, Tianhe, 483 Wushan Road, Guangzhou, 510642, Guangdong, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, 510642, Guangdong, China. FAU - Wang, Weixu AU - Wang W AD - Department of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, Guangdong, China. AD - Guangdong Engineering Research Center for Grassland Science, Tianhe, 483 Wushan Road, Guangzhou, 510642, Guangdong, China. FAU - Liu, Junjie AU - Liu J AD - Department of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, Guangdong, China. AD - Guangdong Engineering Research Center for Grassland Science, Tianhe, 483 Wushan Road, Guangzhou, 510642, Guangdong, China. FAU - Huang, Jinhang AU - Huang J AD - Department of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, Guangdong, China. AD - Guangdong Engineering Research Center for Grassland Science, Tianhe, 483 Wushan Road, Guangzhou, 510642, Guangdong, China. FAU - Huang, Wei AU - Huang W AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, Guangdong, China. AD - College of Life Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong, China. FAU - Ge, Liangfa AU - Ge L AUID- ORCID: 0000-0002-4831-4637 AD - Department of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, Guangdong, China. lge@scau.edu.cn. AD - Guangdong Engineering Research Center for Grassland Science, Tianhe, 483 Wushan Road, Guangzhou, 510642, Guangdong, China. lge@scau.edu.cn. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, 510642, Guangdong, China. lge@scau.edu.cn. LA - eng PT - Journal Article DEP - 20200831 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 SB - IM MH - CYS2-HIS2 Zinc Fingers/*genetics MH - *Gene Duplication MH - Genes, Plant/*genetics MH - *Genome-Wide Association Study MH - Medicago truncatula/*genetics MH - *Multigene Family PMC - PMC7460785 OTO - NOTNLM OT - C2H2 OT - EAR motif OT - Expression OT - Gene family OT - Local gene duplication OT - Zinc finger COIS- The authors declare that they have no competing interests. EDAT- 2020/09/02 06:00 MHDA- 2021/02/20 06:00 PMCR- 2020/08/31 CRDT- 2020/09/02 06:00 PHST- 2019/07/24 00:00 [received] PHST- 2020/08/25 00:00 [accepted] PHST- 2020/09/02 06:00 [entrez] PHST- 2020/09/02 06:00 [pubmed] PHST- 2021/02/20 06:00 [medline] PHST- 2020/08/31 00:00 [pmc-release] AID - 10.1186/s12870-020-02619-6 [pii] AID - 2619 [pii] AID - 10.1186/s12870-020-02619-6 [doi] PST - epublish SO - BMC Plant Biol. 2020 Aug 31;20(1):401. doi: 10.1186/s12870-020-02619-6. ##### PUB RECORD ##### ## 10.1094/MPMI-06-10-0144 20731530 Miyahara, Richens et al., 2010 "Miyahara A, Richens J, Starker C, Morieri G, Smith L, Long S, Downie JA, Oldroyd GE. Conservation in function of a SCAR/WAVE component during infection thread and root hair growth in Medicago truncatula. Mol Plant Microbe Interact. 2010 Dec;23(12):1553-62. doi: 10.1094/MPMI-06-10-0144. PMID: 20731530." ## PMID- 20731530 OWN - NLM STAT- MEDLINE DCOM- 20110111 LR - 20220310 IS - 0894-0282 (Print) IS - 0894-0282 (Linking) VI - 23 IP - 12 DP - 2010 Dec TI - Conservation in function of a SCAR/WAVE component during infection thread and root hair growth in Medicago truncatula. PG - 1553-62 LID - 10.1094/MPMI-06-10-0144 [doi] AB - Nitrogen-fixing symbioses of plants are often associated with bacterially infected nodules where nitrogen fixation occurs. The plant host facilitates bacterial infection with the formation of infection threads, unique structures associated with these symbioses, which are invaginations of the host cell with the capability of traversing cellular junctions. Here, we show that the infection thread shares mechanistic similarities to polar-growing cells, because the required for infection thread (RIT) locus of Medicago truncatula has roles in root-hair, trichome, and infection-thread growth. We show that RIT encodes the M. truncatula ortholog of NAP1, a component of the SCAR/WAVE (suppressor of cAMP receptor/WASP-family verprolin homologous protein) complex that regulates actin polymerization, through the activation of ARP2/3. NAP1 of Arabidopsis thaliana functions equivalently to the M. truncatula gene, indicating that the mode of action of NAP1 is functionally conserved across species and that legumes have not evolved a unique functionality for NAP1 during rhizobial colonization. This work highlights the surprising commonality between polar-growing cells and a polar-growing cellular intrusion and reveals important insights into the formation and maintenance of infection-thread development. FAU - Miyahara, Akira AU - Miyahara A AD - Department of Disease and Stress Biology, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK. FAU - Richens, Jennifer AU - Richens J FAU - Starker, Colby AU - Starker C FAU - Morieri, Giulia AU - Morieri G FAU - Smith, Lucinda AU - Smith L FAU - Long, Sharon AU - Long S FAU - Downie, J Allan AU - Downie JA FAU - Oldroyd, Giles E D AU - Oldroyd GE LA - eng SI - GENBANK/HM590708 GR - BBS/E/J/000CA336/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mol Plant Microbe Interact JT - Molecular plant-microbe interactions : MPMI JID - 9107902 RN - 0 (Plant Proteins) SB - IM MH - Gene Expression Regulation, Plant/*physiology MH - Medicago truncatula/genetics/*metabolism MH - Molecular Sequence Data MH - Mutation MH - Plant Proteins/genetics/*metabolism MH - Plant Root Nodulation/*physiology MH - Plant Roots/*growth & development/physiology MH - Symbiosis EDAT- 2010/08/25 06:00 MHDA- 2011/01/12 06:00 CRDT- 2010/08/25 06:00 PHST- 2010/08/25 06:00 [entrez] PHST- 2010/08/25 06:00 [pubmed] PHST- 2011/01/12 06:00 [medline] AID - 10.1094/MPMI-06-10-0144 [doi] PST - ppublish SO - Mol Plant Microbe Interact. 2010 Dec;23(12):1553-62. doi: 10.1094/MPMI-06-10-0144. ##### PUB RECORD ##### ## 10.3389/fpls.2015.00575 26284091 Chen, Liu et al., 2015 "Chen DS, Liu CW, Roy S, Cousins D, Stacey N, Murray JD. Identification of a core set of rhizobial infection genes using data from single cell-types. Front Plant Sci. 2015 Jul 28;6:575. doi: 10.3389/fpls.2015.00575. PMID: 26284091; PMCID: PMC4517396." ## PMID- 26284091 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20150818 LR - 20220129 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 6 DP - 2015 TI - Identification of a core set of rhizobial infection genes using data from single cell-types. PG - 575 LID - 10.3389/fpls.2015.00575 [doi] LID - 575 AB - Genome-wide expression studies on nodulation have varied in their scale from entire root systems to dissected nodules or root sections containing nodule primordia (NP). More recently efforts have focused on developing methods for isolation of root hairs from infected plants and the application of laser-capture microdissection technology to nodules. Here we analyze two published data sets to identify a core set of infection genes that are expressed in the nodule and in root hairs during infection. Among the genes identified were those encoding phenylpropanoid biosynthesis enzymes including Chalcone-O-Methyltransferase which is required for the production of the potent Nod gene inducer 4',4-dihydroxy-2-methoxychalcone. A promoter-GUS analysis in transgenic hairy roots for two genes encoding Chalcone-O-Methyltransferase isoforms revealed their expression in rhizobially infected root hairs and the nodule infection zone but not in the nitrogen fixation zone. We also describe a group of Rhizobially Induced Peroxidases whose expression overlaps with the production of superoxide in rhizobially infected root hairs and in nodules and roots. Finally, we identify a cohort of co-regulated transcription factors as candidate regulators of these processes. FAU - Chen, Da-Song AU - Chen DS AD - State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan China. FAU - Liu, Cheng-Wu AU - Liu CW AD - John Innes Centre, Department of Cell and Developmental Biology, Norfolk UK. FAU - Roy, Sonali AU - Roy S AD - John Innes Centre, Department of Cell and Developmental Biology, Norfolk UK. FAU - Cousins, Donna AU - Cousins D AD - John Innes Centre, Department of Cell and Developmental Biology, Norfolk UK. FAU - Stacey, Nicola AU - Stacey N AD - John Innes Centre, Department of Cell and Developmental Biology, Norfolk UK. FAU - Murray, Jeremy D AU - Murray JD AD - John Innes Centre, Department of Cell and Developmental Biology, Norfolk UK. LA - eng GR - BB/G023832/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article DEP - 20150728 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC4517396 OTO - NOTNLM OT - CCAAT-box OT - Nod factors OT - infection threads OT - infection zone OT - medicarpin OT - methoxychalcone OT - nod genes OT - nodulation EDAT- 2015/08/19 06:00 MHDA- 2015/08/19 06:01 PMCR- 2015/01/01 CRDT- 2015/08/19 06:00 PHST- 2015/05/12 00:00 [received] PHST- 2015/07/13 00:00 [accepted] PHST- 2015/08/19 06:00 [entrez] PHST- 2015/08/19 06:00 [pubmed] PHST- 2015/08/19 06:01 [medline] PHST- 2015/01/01 00:00 [pmc-release] AID - 10.3389/fpls.2015.00575 [doi] PST - epublish SO - Front Plant Sci. 2015 Jul 28;6:575. doi: 10.3389/fpls.2015.00575. eCollection 2015. ##### PUB RECORD ##### ## 10.1104/pp.105.069054 16244141 Isayenkov, Mrosk et al., 2005 "Isayenkov S, Mrosk C, Stenzel I, Strack D, Hause B. Suppression of allene oxide cyclase in hairy roots of Medicago truncatula reduces jasmonate levels and the degree of mycorrhization with Glomus intraradices. Plant Physiol. 2005 Nov;139(3):1401-10. doi: 10.1104/pp.105.069054. Epub 2005 Oct 21. PMID: 16244141; PMCID: PMC1283775." ## PMID- 16244141 OWN - NLM STAT- MEDLINE DCOM- 20060209 LR - 20240314 IS - 0032-0889 (Print) IS - 1532-2548 (Electronic) IS - 0032-0889 (Linking) VI - 139 IP - 3 DP - 2005 Nov TI - Suppression of allene oxide cyclase in hairy roots of Medicago truncatula reduces jasmonate levels and the degree of mycorrhization with Glomus intraradices. PG - 1401-10 AB - During the symbiotic interaction between Medicago truncatula and the arbuscular mycorrhizal (AM) fungus Glomus intraradices, an endogenous increase in jasmonic acid (JA) occurs. Two full-length cDNAs coding for the JA-biosynthetic enzyme allene oxide cyclase (AOC) from M. truncatula, designated as MtAOC1 and MtAOC2, were cloned and characterized. The AOC protein was localized in plastids and found to occur constitutively in all vascular tissues of M. truncatula. In leaves and roots, MtAOCs are expressed upon JA application. Enhanced expression was also observed during mycorrhization with G. intraradices. A partial suppression of MtAOC expression was achieved in roots following transformation with Agrobacterium rhizogenes harboring the MtAOC1 cDNA in the antisense direction under control of the cauliflower mosaic virus 35S promoter. In comparison to samples transformed with 35SuidA, roots with suppressed MtAOC1 expression exhibited lower JA levels and a remarkable delay in the process of colonization with G. intraradices. Both the mycorrhization rate, quantified by fungal rRNA, and the arbuscule formation, analyzed by the expression level of the AM-specific gene MtPT4, were affected. Staining of fungal material in roots with suppressed MtAOC1 revealed a decreased number of arbuscules, but these did not exhibit an altered structure. Our results indicate a crucial role for JA in the establishment of AM symbiosis. FAU - Isayenkov, Stanislav AU - Isayenkov S AD - Department of Secondary Metabolism , Leibniz Institute of Plant Biochemistry, D-06120 Halle , Germany. FAU - Mrosk, Cornelia AU - Mrosk C FAU - Stenzel, Irene AU - Stenzel I FAU - Strack, Dieter AU - Strack D FAU - Hause, Bettina AU - Hause B LA - eng SI - GENBANK/AJ308489 SI - GENBANK/AJ866733 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20051021 PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 RN - 0 (Cyclopentanes) RN - 0 (DNA, Complementary) RN - 0 (Oxylipins) RN - 0 (RNA, Messenger) RN - 6RI5N05OWW (jasmonic acid) RN - EC 5.3.- (Intramolecular Oxidoreductases) RN - EC 5.3.99.6 (hydroperoxide isomerase) SB - IM MH - Cyclopentanes/*metabolism/pharmacology MH - DNA, Complementary/genetics MH - Gene Expression Profiling MH - Gene Expression Regulation, Developmental MH - Intramolecular Oxidoreductases/*antagonists & inhibitors/genetics/metabolism MH - Medicago truncatula/*enzymology/microbiology MH - Molecular Sequence Data MH - Mycorrhizae/cytology/drug effects/*metabolism MH - Organ Specificity MH - Oxylipins MH - Plant Leaves/cytology/drug effects MH - Plant Roots/cytology/drug effects/*enzymology MH - Plants, Genetically Modified MH - Protein Transport MH - RNA, Messenger/genetics/metabolism MH - Symbiosis MH - Transformation, Genetic PMC - PMC1283775 EDAT- 2005/10/26 09:00 MHDA- 2006/02/10 09:00 PMCR- 2006/11/01 CRDT- 2005/10/26 09:00 PHST- 2005/10/26 09:00 [pubmed] PHST- 2006/02/10 09:00 [medline] PHST- 2005/10/26 09:00 [entrez] PHST- 2006/11/01 00:00 [pmc-release] AID - pp.105.069054 [pii] AID - 069054 [pii] AID - 10.1104/pp.105.069054 [doi] PST - ppublish SO - Plant Physiol. 2005 Nov;139(3):1401-10. doi: 10.1104/pp.105.069054. Epub 2005 Oct 21. ##### PUB RECORD ##### ## 10.1111/j.1469-8137.2011.03718.x 21679315 Godiard, Lepage et al., 2011 "Godiard L, Lepage A, Moreau S, Laporte D, Verdenaud M, Timmers T, Gamas P. MtbHLH1, a bHLH transcription factor involved in Medicago truncatula nodule vascular patterning and nodule to plant metabolic exchanges. New Phytol. 2011 Jul;191(2):391-404. doi: 10.1111/j.1469-8137.2011.03718.x. Epub 2011 Jun 17. PMID: 21679315; PMCID: PMC3206218." ## PMID- 21679315 OWN - NLM STAT- MEDLINE DCOM- 20130328 LR - 20240320 IS - 1469-8137 (Electronic) IS - 0028-646X (Print) IS - 0028-646X (Linking) VI - 191 IP - 2 DP - 2011 Jul TI - MtbHLH1, a bHLH transcription factor involved in Medicago truncatula nodule vascular patterning and nodule to plant metabolic exchanges. PG - 391-404 LID - 10.1111/j.1469-8137.2011.03718.x [doi] AB - This study aimed at defining the role of a basic helix-loop-helix (bHLH) transcription factor gene from Medicago truncatula, MtbHLH1, whose expression is upregulated during the development of root nodules produced upon infection by rhizobia bacteria. We used MtbHLH1 promoter::GUS fusions and quantitative reverse-transcription polymerase chain reaction analyses to finely characterize the MtbHLH1 expression pattern. We altered MtbHLH1 function by expressing a dominantly repressed construct (CRES-T approach) and looked for possible MtbHLH1 target genes by transcriptomics. We found that MtbHLH1 is expressed in nodule primordia cells derived from pericycle divisions, in nodule vascular bundles (VBs) and in uninfected cells of the nitrogen (N) fixation zone. MtbHLH1 is also expressed in root tips, lateral root primordia cells and root VBs, and induced upon auxin treatment. Altering MtbHLH1 function led to an unusual phenotype, with a modified patterning of nodule VB development and a reduced growth of aerial parts of the plant, even though the nodules were able to fix atmospheric N. Several putative MtbHLH1 regulated genes were identified, including an asparagine synthase and a LOB (lateral organ boundary) transcription factor. Our results suggest that the MtbHLH1 gene is involved in the control of nodule vasculature patterning and nutrient exchanges between nodules and roots. CI - (c) 2011 The Authors. New Phytologist (c) 2011 New Phytologist Trust. FAU - Godiard, Laurence AU - Godiard L AD - Laboratoire des Interactions Plantes Microorganismes, Unite Mixte de Recherche, Institut National de la Recherche Agronomique - Centre National de la Recherche Scientifique 441/2594, F-31320 Castanet Tolosan, France. FAU - Lepage, Agnes AU - Lepage A AD - Laboratoire des Interactions Plantes Microorganismes, Unite Mixte de Recherche, Institut National de la Recherche Agronomique - Centre National de la Recherche Scientifique 441/2594, F-31320 Castanet Tolosan, France. FAU - Moreau, Sandra AU - Moreau S AD - Laboratoire des Interactions Plantes Microorganismes, Unite Mixte de Recherche, Institut National de la Recherche Agronomique - Centre National de la Recherche Scientifique 441/2594, F-31320 Castanet Tolosan, France. FAU - Laporte, Damien AU - Laporte D AD - Jian-Qiu Wu's laboratory, Ohio State University, 612 Biosciences Building, 484 W 12th Ave, Columbus, OH 43210, USA. FAU - Verdenaud, Marion AU - Verdenaud M AD - Laboratoire des Interactions Plantes Microorganismes, Unite Mixte de Recherche, Institut National de la Recherche Agronomique - Centre National de la Recherche Scientifique 441/2594, F-31320 Castanet Tolosan, France. FAU - Timmers, Ton AU - Timmers T AD - Laboratoire des Interactions Plantes Microorganismes, Unite Mixte de Recherche, Institut National de la Recherche Agronomique - Centre National de la Recherche Scientifique 441/2594, F-31320 Castanet Tolosan, France. FAU - Gamas, Pascal AU - Gamas P AD - Laboratoire des Interactions Plantes Microorganismes, Unite Mixte de Recherche, Institut National de la Recherche Agronomique - Centre National de la Recherche Scientifique 441/2594, F-31320 Castanet Tolosan, France. LA - eng SI - GENBANK/FR697055 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20110617 PL - England TA - New Phytol JT - The New phytologist JID - 9882884 RN - 0 (Basic Helix-Loop-Helix Transcription Factors) RN - 0 (Indoleacetic Acids) RN - 0 (Plant Growth Regulators) RN - 0 (Plant Proteins) SB - IM MH - Basic Helix-Loop-Helix Transcription Factors/genetics/*metabolism MH - Body Patterning MH - Gene Expression Profiling MH - Gene Expression Regulation, Plant/drug effects MH - Indoleacetic Acids/pharmacology MH - Medicago truncatula/genetics/*metabolism/microbiology/physiology MH - Molecular Sequence Data MH - Nitrogen Fixation/genetics MH - Phenotype MH - Plant Growth Regulators/pharmacology MH - Plant Proteins/genetics/metabolism MH - Plant Root Nodulation MH - Plants, Genetically Modified MH - Promoter Regions, Genetic MH - Rhizobium/genetics/*physiology MH - Root Nodules, Plant/genetics/growth & development/*metabolism MH - Symbiosis/genetics PMC - PMC3206218 EDAT- 2011/06/18 06:00 MHDA- 2013/03/30 06:00 CRDT- 2011/06/18 06:00 PHST- 2011/06/18 06:00 [entrez] PHST- 2011/06/18 06:00 [pubmed] PHST- 2013/03/30 06:00 [medline] AID - 10.1111/j.1469-8137.2011.03718.x [doi] PST - ppublish SO - New Phytol. 2011 Jul;191(2):391-404. doi: 10.1111/j.1469-8137.2011.03718.x. Epub 2011 Jun 17. ##### PUB RECORD ##### ## 10.1371/journal.pgen.1004891 25521478 Huault, Laffont et al., 2014 "Huault E, Laffont C, Wen J, Mysore KS, Ratet P, Duc G, Frugier F. Local and systemic regulation of plant root system architecture and symbiotic nodulation by a receptor-like kinase. PLoS Genet. 2014 Dec 18;10(12):e1004891. doi: 10.1371/journal.pgen.1004891. PMID: 25521478; PMCID: PMC4270686." ## PMID- 25521478 OWN - NLM STAT- MEDLINE DCOM- 20151106 LR - 20181113 IS - 1553-7404 (Electronic) IS - 1553-7390 (Print) IS - 1553-7390 (Linking) VI - 10 IP - 12 DP - 2014 Dec TI - Local and systemic regulation of plant root system architecture and symbiotic nodulation by a receptor-like kinase. PG - e1004891 LID - 10.1371/journal.pgen.1004891 [doi] LID - e1004891 AB - In plants, root system architecture is determined by the activity of root apical meristems, which control the root growth rate, and by the formation of lateral roots. In legumes, an additional root lateral organ can develop: the symbiotic nitrogen-fixing nodule. We identified in Medicago truncatula ten allelic mutants showing a compact root architecture phenotype (cra2) independent of any major shoot phenotype, and that consisted of shorter roots, an increased number of lateral roots, and a reduced number of nodules. The CRA2 gene encodes a Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) that primarily negatively regulates lateral root formation and positively regulates symbiotic nodulation. Grafting experiments revealed that CRA2 acts through different pathways to regulate these lateral organs originating from the roots, locally controlling the lateral root development and nodule formation systemically from the shoots. The CRA2 LRR-RLK therefore integrates short- and long-distance regulations to control root system architecture under non-symbiotic and symbiotic conditions. FAU - Huault, Emeline AU - Huault E AD - Institut des Sciences du Vegetal (ISV), CNRS, UPR2355, Gif-sur-Yvette, France. FAU - Laffont, Carole AU - Laffont C AD - Institut des Sciences du Vegetal (ISV), CNRS, UPR2355, Gif-sur-Yvette, France. FAU - Wen, Jiangqi AU - Wen J AD - Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, United States of America. FAU - Mysore, Kirankumar S AU - Mysore KS AD - Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, United States of America. FAU - Ratet, Pascal AU - Ratet P AD - Institut des Sciences du Vegetal (ISV), CNRS, UPR2355, Gif-sur-Yvette, France. FAU - Duc, Gerard AU - Duc G AD - INRA, UMR1347 Agroecologie, Dijon, France. FAU - Frugier, Florian AU - Frugier F AD - Institut des Sciences du Vegetal (ISV), CNRS, UPR2355, Gif-sur-Yvette, France. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141218 PL - United States TA - PLoS Genet JT - PLoS genetics JID - 101239074 RN - 0 (Plant Proteins) RN - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) SB - IM MH - Medicago truncatula/*genetics/growth & development/microbiology MH - Meristem/genetics/growth & development/microbiology MH - Phylogeny MH - Plant Proteins/*physiology MH - Receptor Protein-Tyrosine Kinases/*physiology MH - Rhizobium/physiology MH - Root Nodules, Plant/*genetics/growth & development/microbiology MH - Symbiosis PMC - PMC4270686 COIS- The authors have declared that no competing interests exist. EDAT- 2014/12/19 06:00 MHDA- 2015/11/07 06:00 PMCR- 2014/12/18 CRDT- 2014/12/19 06:00 PHST- 2014/06/05 00:00 [received] PHST- 2014/11/13 00:00 [accepted] PHST- 2014/12/19 06:00 [entrez] PHST- 2014/12/19 06:00 [pubmed] PHST- 2015/11/07 06:00 [medline] PHST- 2014/12/18 00:00 [pmc-release] AID - PGENETICS-D-14-01490 [pii] AID - 10.1371/journal.pgen.1004891 [doi] PST - epublish SO - PLoS Genet. 2014 Dec 18;10(12):e1004891. doi: 10.1371/journal.pgen.1004891. eCollection 2014 Dec. ##### PUB RECORD ##### ## 10.1242/dev.158766 29361570 Cheng, Li et al., 2018 "Cheng X, Li G, Tang Y, Wen J. Dissection of genetic regulation of compound inflorescence development in Medicago truncatula. Development. 2018 Feb 8;145(3):dev158766. doi: 10.1242/dev.158766. PMID: 29361570." ## PMID- 29361570 OWN - NLM STAT- MEDLINE DCOM- 20180409 LR - 20180409 IS - 1477-9129 (Electronic) IS - 0950-1991 (Linking) VI - 145 IP - 3 DP - 2018 Feb 8 TI - Dissection of genetic regulation of compound inflorescence development in Medicago truncatula. LID - dev158766 [pii] LID - 10.1242/dev.158766 [doi] AB - Development of inflorescence architecture is controlled by genetic regulatory networks. TERMINAL FLOWER1 (TFL1), APETALA1 (AP1), LEAFY (LFY) and FRUITFULL (FUL) are core regulators for inflorescence development. To understand the regulation of compound inflorescence development, we characterized mutants of corresponding orthologous genes, MtTFL1, MtAP1, SINGLE LEAFLET1 (SGL1) and MtFULc, in Medicago truncatula, and analyzed expression patterns of these genes. Results indicate that MtTFL1, MtFULc, MtAP1 and SGL1 play specific roles in identity determination of primary inflorescence meristems, secondary inflorescence meristems, floral meristems and common primordia, respectively. Double mutation of MtTFL1 and MtFULc transforms compound inflorescences to simple flowers, whereas single mutation of MtTFL1 changes the inflorescence branching pattern from monopodial to sympodial. Double mutant mtap1sgl1 completely loses floral meristem identity. We conclude that inflorescence architecture in M. truncatula is controlled by spatiotemporal expression of MtTFL1, MtFULc, MtAP1 and SGL1 through reciprocal repression. Although this regulatory network shares similarity with the pea model, it has specificity in regulating inflorescence architecture in Mtruncatula This study establishes M. truncatula as an excellent genetic model for understanding compound inflorescence development in related legume crops. CI - (c) 2018. Published by The Company of Biologists Ltd. FAU - Cheng, Xiaofei AU - Cheng X AD - Noble Research Institute, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA. FAU - Li, Guifen AU - Li G AD - Noble Research Institute, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA. FAU - Tang, Yuhong AU - Tang Y AD - Noble Research Institute, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA. FAU - Wen, Jiangqi AU - Wen J AUID- ORCID: 0000-0001-5113-7750 AD - Noble Research Institute, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA jwen@noble.org. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180208 PL - England TA - Development JT - Development (Cambridge, England) JID - 8701744 RN - 0 (Plant Proteins) RN - 0 (RNA, Plant) SB - IM MH - Gene Expression Profiling MH - Gene Expression Regulation, Developmental MH - Gene Expression Regulation, Plant MH - Genes, Plant MH - Inflorescence/*genetics/*growth & development/metabolism MH - Medicago truncatula/*genetics/*growth & development/metabolism MH - Meristem/genetics/growth & development/metabolism MH - Microscopy, Electron, Scanning MH - Models, Genetic MH - Mutation MH - Plant Proteins/genetics/metabolism MH - Plants, Genetically Modified MH - RNA, Plant/genetics/metabolism OTO - NOTNLM OT - Development OT - Genetic regulation OT - Inflorescence OT - Legume OT - Medicago truncatula COIS- Competing interestsThe authors declare no competing or financial interests. EDAT- 2018/01/24 06:00 MHDA- 2018/04/10 06:00 CRDT- 2018/01/24 06:00 PHST- 2017/08/22 00:00 [received] PHST- 2018/01/02 00:00 [accepted] PHST- 2018/01/24 06:00 [pubmed] PHST- 2018/04/10 06:00 [medline] PHST- 2018/01/24 06:00 [entrez] AID - dev.158766 [pii] AID - 10.1242/dev.158766 [doi] PST - epublish SO - Development. 2018 Feb 8;145(3):dev158766. doi: 10.1242/dev.158766. ##### PUB RECORD ##### ## 10.1038/s41477-018-0286-7 30397259 Pecrix, Staton et al., 2018 "Pecrix Y, Staton SE, Sallet E, Lelandais-Brière C, Moreau S, Carrère S, Blein T, Jardinaud MF, Latrasse D, Zouine M, Zahm M, Kreplak J, Mayjonade B, Satgé C, Perez M, Cauet S, Marande W, Chantry-Darmon C, Lopez-Roques C, Bouchez O, Bérard A, Debellé F, Muños S, Bendahmane A, Bergès H, Niebel A, Buitink J, Frugier F, Benhamed M, Crespi M, Gouzy J, Gamas P. Whole-genome landscape of Medicago truncatula symbiotic genes. Nat Plants. 2018 Dec;4(12):1017-1025. doi: 10.1038/s41477-018-0286-7. Epub 2018 Nov 5. PMID: 30397259." ## PMID- 30397259 OWN - NLM STAT- MEDLINE DCOM- 20190701 LR - 20191029 IS - 2055-0278 (Electronic) IS - 2055-0278 (Linking) VI - 4 IP - 12 DP - 2018 Dec TI - Whole-genome landscape of Medicago truncatula symbiotic genes. PG - 1017-1025 LID - 10.1038/s41477-018-0286-7 [doi] AB - Advances in deciphering the functional architecture of eukaryotic genomes have been facilitated by recent breakthroughs in sequencing technologies, enabling a more comprehensive representation of genes and repeat elements in genome sequence assemblies, as well as more sensitive and tissue-specific analyses of gene expression. Here we show that PacBio sequencing has led to a substantially improved genome assembly of Medicago truncatula A17, a legume model species notable for endosymbiosis studies(1), and has enabled the identification of genome rearrangements between genotypes at a near-base-pair resolution. Annotation of the new M. truncatula genome sequence has allowed for a thorough analysis of transposable elements and their dynamics, as well as the identification of new players involved in symbiotic nodule development, in particular 1,037 upregulated long non-coding RNAs (lncRNAs). We have also discovered that a substantial proportion (~35% and 38%, respectively) of the genes upregulated in nodules or expressed in the nodule differentiation zone colocalize in genomic clusters (270 and 211, respectively), here termed symbiotic islands. These islands contain numerous expressed lncRNA genes and display differentially both DNA methylation and histone marks. Epigenetic regulations and lncRNAs are therefore attractive candidate elements for the orchestration of symbiotic gene expression in the M. truncatula genome. FAU - Pecrix, Yann AU - Pecrix Y AUID- ORCID: 0000-0002-6537-3145 AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Staton, S Evan AU - Staton SE AUID- ORCID: 0000-0002-5681-6047 AD - University of British Columbia, Vancouver, Canada. FAU - Sallet, Erika AU - Sallet E AUID- ORCID: 0000-0003-4637-473X AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Lelandais-Briere, Christine AU - Lelandais-Briere C AD - IPS2, CNRS, INRA, Universities of Paris Diderot and Sorbonne Paris Cite, Gif sur Yvette, France. AD - IPS2, CNRS, INRA, Universities of Paris Diderot, Paris Sud, Evry and Paris-Saclay, Gif sur Yvette, France. FAU - Moreau, Sandra AU - Moreau S AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Carrere, Sebastien AU - Carrere S AUID- ORCID: 0000-0002-2348-0778 AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Blein, Thomas AU - Blein T AD - IPS2, CNRS, INRA, Universities of Paris Diderot and Sorbonne Paris Cite, Gif sur Yvette, France. AD - IPS2, CNRS, INRA, Universities of Paris Diderot, Paris Sud, Evry and Paris-Saclay, Gif sur Yvette, France. FAU - Jardinaud, Marie-Francoise AU - Jardinaud MF AD - LIPM, Universite de Toulouse, INPT, ENSAT, Castanet-Tolosan, France. FAU - Latrasse, David AU - Latrasse D AD - IPS2, CNRS, INRA, Universities of Paris Diderot and Sorbonne Paris Cite, Gif sur Yvette, France. AD - IPS2, CNRS, INRA, Universities of Paris Diderot, Paris Sud, Evry and Paris-Saclay, Gif sur Yvette, France. FAU - Zouine, Mohamed AU - Zouine M AD - GBF, Universite de Toulouse, INPT, ENSAT, Castanet-Tolosan, France. FAU - Zahm, Margot AU - Zahm M AD - GBF, Universite de Toulouse, INPT, ENSAT, Castanet-Tolosan, France. FAU - Kreplak, Jonathan AU - Kreplak J AD - AGROECOLOGIE, INRA, Dijon, France. FAU - Mayjonade, Baptiste AU - Mayjonade B AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Satge, Carine AU - Satge C AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. AD - CNRGV, INRA, Castanet-Tolosan, France. FAU - Perez, Magali AU - Perez M AD - IPS2, CNRS, INRA, Universities of Paris Diderot and Sorbonne Paris Cite, Gif sur Yvette, France. AD - IPS2, CNRS, INRA, Universities of Paris Diderot, Paris Sud, Evry and Paris-Saclay, Gif sur Yvette, France. FAU - Cauet, Stephane AU - Cauet S AD - CNRGV, INRA, Castanet-Tolosan, France. FAU - Marande, William AU - Marande W AD - CNRGV, INRA, Castanet-Tolosan, France. FAU - Chantry-Darmon, Celine AU - Chantry-Darmon C AD - CNRGV, INRA, Castanet-Tolosan, France. FAU - Lopez-Roques, Celine AU - Lopez-Roques C AD - INRA, US1426, GeT-PlaGe, Genotoul, Castanet-Tolosan, France. FAU - Bouchez, Olivier AU - Bouchez O AD - INRA, US1426, GeT-PlaGe, Genotoul, Castanet-Tolosan, France. FAU - Berard, Aurelie AU - Berard A AD - INRA, US 1279 EPGV, Universite Paris-Saclay, Evry, France. FAU - Debelle, Frederic AU - Debelle F AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Munos, Stephane AU - Munos S AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Bendahmane, Abdelhafid AU - Bendahmane A AD - IPS2, CNRS, INRA, Universities of Paris Diderot and Sorbonne Paris Cite, Gif sur Yvette, France. AD - IPS2, CNRS, INRA, Universities of Paris Diderot, Paris Sud, Evry and Paris-Saclay, Gif sur Yvette, France. FAU - Berges, Helene AU - Berges H AD - CNRGV, INRA, Castanet-Tolosan, France. FAU - Niebel, Andreas AU - Niebel A AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. FAU - Buitink, Julia AU - Buitink J AUID- ORCID: 0000-0002-1457-764X AD - IRHS, Agrocampus-Ouest, INRA, Universite d'Angers, Beaucouze, France. FAU - Frugier, Florian AU - Frugier F AD - IPS2, CNRS, INRA, Universities of Paris Diderot and Sorbonne Paris Cite, Gif sur Yvette, France. AD - IPS2, CNRS, INRA, Universities of Paris Diderot, Paris Sud, Evry and Paris-Saclay, Gif sur Yvette, France. FAU - Benhamed, Moussa AU - Benhamed M AD - IPS2, CNRS, INRA, Universities of Paris Diderot and Sorbonne Paris Cite, Gif sur Yvette, France. AD - IPS2, CNRS, INRA, Universities of Paris Diderot, Paris Sud, Evry and Paris-Saclay, Gif sur Yvette, France. FAU - Crespi, Martin AU - Crespi M AD - IPS2, CNRS, INRA, Universities of Paris Diderot and Sorbonne Paris Cite, Gif sur Yvette, France. AD - IPS2, CNRS, INRA, Universities of Paris Diderot, Paris Sud, Evry and Paris-Saclay, Gif sur Yvette, France. FAU - Gouzy, Jerome AU - Gouzy J AUID- ORCID: 0000-0001-5695-4557 AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. Jerome.Gouzy@inra.fr. FAU - Gamas, Pascal AU - Gamas P AUID- ORCID: 0000-0002-6253-4249 AD - LIPM, Universite de Toulouse, INRA, CNRS, Castanet-Tolosan, France. Pascal.Gamas@inra.fr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181105 PL - England TA - Nat Plants JT - Nature plants JID - 101651677 RN - 0 (Plant Proteins) RN - 0 (RNA, Plant) RN - 0 (RNA, Untranslated) SB - IM MH - DNA Methylation MH - *Epigenesis, Genetic MH - Gene Expression Regulation, Plant MH - Genome, Plant/*genetics MH - Genomics MH - Medicago truncatula/*genetics MH - Multigene Family MH - Plant Proteins/genetics MH - RNA, Plant/genetics MH - RNA, Untranslated/*genetics MH - Root Nodules, Plant/genetics MH - Symbiosis/*genetics EDAT- 2018/11/07 06:00 MHDA- 2019/07/02 06:00 CRDT- 2018/11/07 06:00 PHST- 2018/03/07 00:00 [received] PHST- 2018/09/21 00:00 [accepted] PHST- 2018/11/07 06:00 [pubmed] PHST- 2019/07/02 06:00 [medline] PHST- 2018/11/07 06:00 [entrez] AID - 10.1038/s41477-018-0286-7 [pii] AID - 10.1038/s41477-018-0286-7 [doi] PST - ppublish SO - Nat Plants. 2018 Dec;4(12):1017-1025. doi: 10.1038/s41477-018-0286-7. Epub 2018 Nov 5.